A5021218816- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Antibiotic Resistance in Bacteria
- Metabolomics and Mass Spectrometry Studies
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
- Veterinary medicine and infectious diseases
- Pluripotent Stem Cells Research
- Folate and B Vitamins Research
- Endoplasmic Reticulum Stress and Disease
- Birth, Development, and Health
- Cell Image Analysis Techniques
- Autophagy in Disease and Therapy
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Biochemical Acid Research Studies
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Aging, Elder Care, and Social Issues
École Normale Supérieure de Lyon
2014-2023
Institut de Génomique Fonctionnelle de Lyon
2023
Université Claude Bernard Lyon 1
2014-2021
Centre National de la Recherche Scientifique
2012-2021
Laboratoire de Biologie et Modélisation de la Cellule
2014-2021
Hospices Civils de Lyon
2014-2021
Inserm
2021
Laboratoire de Photochimie et d'Ingénierie Macromoléculaire
2015
Laboratoire d'Ingénierie des Systèmes Macromoléculaires
2013-2015
Aix-Marseille Université
2012-2014
Campylobacter jejuni , a Gram‐negative bacterium, is common cause of gastrointestinal disease. By analogy with other enteric pathogens such as Salmonella and Shigella the ability C. to bind host cells thought be essential in pathogenesis enteritis. Scanning electron microscopy infected INT407 suggested that bound component extracellular matrix. Binding assays using immobilized matrix proteins soluble fibronectin showed specific saturable binding . Ligand immunoblot 125 I‐labelled revealed an...
Presented here is the first evidence that Campylobacter jejuni secrete proteins upon co‐cultivation with host cells and in INT 407 cell‐conditioned medium. A C. gene designated ciaB for invasion antigen B was identified, using a differential screening technique, which required this secretion process efficient entry of bacterium into cell. The encodes protein 610 amino acids calculated molecular mass 73 154 Da. deduced acid sequence CiaB shares similarity type III secreted associated from...
ABSTRACT Campylobacter jejuni and coli are common causes of gastroenteritis in humans. Infection with C. or is commonly acquired by eating undercooked chicken. The goal this study was to develop specific detection assays for isolates based on the cadF virulence gene its product. from encodes a 37-kDa outer membrane protein that promotes binding these pathogens intestinal epithelial cells. A fragment approximately 400 bp amplified 38 40 (95%) 5 6 (83.3%) primers designed amplify an internal...
The genome of Pseudomonas aeruginosa PAO1 contains three type VI secretion systems (T6SSs) called H1-, H2-, and H3-T6SS. H1-T6SS secretes identified toxins that target other bacteria, providing a fitness advantage for P. aeruginosa, likely contributes to bacterial pathogenesis in chronic infections. However, no specific substrates or defined roles have been described the two systems. Here, we demonstrate expression H2-T6SS genes strain is up-regulated during transition from exponential...
Pseudomonas aeruginosa is an opportunistic human pathogen that causes infections in a variety of animal and plant hosts. Caenorhabditis elegans simple model with which one can identify bacterial virulence genes. Previous studies C. have shown depending on the growth medium, P. provokes different pathologies: slow or fast killing, lethal paralysis red death. In this study, we developed high-throughput semi-automated liquid-based assay such entire genome readily be scanned for genes short time...
After uptake by murine macrophages, Salmonella typhimurium is able to survive and replicate within specialized phagosomes called Salmonella-containing vacuoles (SCVs), which are segregated from the late endocytic pathway. The molecular basis of this process virulence factors required not fully understood. In study, we used confocal fluorescence microscopy evaluate interactions between pathway macrophage cell line RAW 264.7 different S. strains. analysis was carried out using fluid-phase...
Summary The binding of Campylobacter jejuni to fibronectin (Fn), a component the extracellular matrix, is mediated by 37 kDa outer membrane protein termed CadF for adhesion Fn. Previous studies have indicated that C. binds Fn on basolateral surface T84 human colonic cells. To further characterize interaction with Fn, enzyme‐linked immunosorbent assays were performed identify Fn‐binding domain (Fn‐BD). Using overlapping 30‐mer and 16‐mer peptides derived from translated cadF nucleotide...
Three Type VI Secretion System (T6SS) loci called H1- to H3-T6SS coexist in Pseudomonas aeruginosa. H1-T6SS targets prokaryotic cells whereas H2-T6SS mediates interactions with both eukaryotic and host cells. Little is known about the third system, except that it may be connected during infection. Here we show required for P. aeruginosa PAO1 virulence worm model. We demonstrate two putative operons, "left" "right", are coregulated by Las Rhl Quorum Sensing systems. Interestingly, RpoN σ54...
Histone H3 Lys 4 methylation (H3K4me) is deposited by the conserved SET1/MLL methyltransferases acting in multiprotein complexes, including Ash2 and Wdr5. Although individual subunits contribute to complex activity, how they influence gene expression specific tissues remains largely unknown. In Caenorhabditis elegans, SET-2/SET1, WDR-5.1, ASH-2 are differentially required for germline H3K4 methylation. Using profiling on germlines from animals lacking set-2, ash-2, or wdr-5.1, we show that...
Disulfide bond formation is required for the folding of many bacterial virulence factors. However, whereas Escherichia coli disulfide bond-forming system well characterized, not much known on pathways that oxidatively fold proteins in pathogenic bacteria. Here, we report detailed unraveling pathway introduces bonds periplasm human pathogen Pseudomonas aeruginosa. The genome P. aeruginosa uniquely encodes two DsbA (P. DsbA1 [PaDsbA1] and PaDsbA2) DsbB (PaDsbB1 PaDsbB2). We found PaDsbA1,...
Significance Organisms must be able to respond quickly stress maintain homeostasis and survive. Perturbation of normal endoplasmic reticulum (ER) function leads the induction evolutionarily conserved unfolded protein response (UPR), essential for recovering cellular homeostasis, or if recovery is impossible, leading cell death. Chromatin-associated proteins have widespread effects on gene regulation, which are particularly important in conditions. We describe a regulatory link between UPR...
Replication of Salmonella typhimurium in host cells depends part on the action Pathogenicity Island 2 (SPI-2) type III secretion system (TTSS), which translocates bacterial effector proteins across membrane Salmonella-containing vacuole (SCV). We have shown previously that one activity SPI-2 TTSS is assembly a coat F-actin vicinity microcolonies. To identify involved dependent actin polymerization, we tested strains carrying mutations each several genes whose products are proposed to be...
Abstract Bacterial pathogens often subvert the innate immune system to establish a successful infection. The direct inhibition of downstream components pathways is particularly well documented but how bacteria interfere with receptor proximal events far less understood. Here, we describe Toll/interleukin 1 ( TIR ) domain‐containing protein (PumA) multi‐drug resistant Pseudomonas aeruginosa PA 7 strain. We found that PumA essential for virulence and inhibits NF ‐κB, property transferable...
A Staphylococcus aureus gene originally identified by signature-tagged mutagenesis as being required for virulence was cloned, sequenced and named svrA. Hydropathy profiles revealed that SvrA is likely to be membrane associated, having two regions with six membrane-spanning domains, the separated an extended hydrophilic loop. When compared wild-type strain, svrA mutant expressed greatly reduced amounts of α-, β- δ-toxins increased amount protein A. Toxin production strain restored levels...
Pseudomonas aeruginosa is an opportunistic human pathogen implicated in nosocomial infection and infecting people with compromised immune systems such as cystic fibrosis patients. Although multiple genes involved P. pathogenesis have been characterized, the overall mechanism of virulence not fully understood. In this study, we identified a functional two-partner secretion (TPS) system, composed PdtA exoprotein its cognate pore-forming β-barrel PdtB transporter, which aeruginosa. We found...
Chromatin regulators contribute to the maintenance of germline transcriptional program. In absence SET-2, Caenorhabditis elegans homolog SET1/COMPASS H3 Lys4 (H3K4) methyltransferase, animals show transgenerational loss identity, leading sterility. To identify signatures associated with progressive fertility, we performed expression profiling set-2 mutant germlines across generations. We a subset genes whose misexpression is first observed in early generations, step refer as priming; their...
Pseudomonas aeruginosa is a human opportunistic pathogen that causes mortality in cystic fibrosis and immunocompromised patients. While many virulence factors of this have already been identified, several remain to be discovered. In respect we set an unprecedented genome-wide screen P. expression library based on yeast growth phenotype. 51 candidates were selected three-round screening process. The robustness the was validated by selection three well known secreted proteins including one...
Campylobacter jejuni, a gram-negative, microaerophilic, spiral bacterium, is common cause of human gastrointestinal disease. Although investigators commonly use C. jejuni glycine-hydrochloride extracts in assays to determine the products that promote binding organism eukaryotic cells, proteins contained within these remain ill defined. Characterization will provide better understanding gene regulation and organization. An antiserum was raised against 29-kDa gel-purified protein detected...