A5013019349- Hippo pathway signaling and YAP/TAZ
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Retinal Development and Disorders
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- RNA regulation and disease
- Glaucoma and retinal disorders
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Cellular transport and secretion
- Hepatitis C virus research
- dental development and anomalies
- Hepatitis B Virus Studies
- Bone and Dental Protein Studies
- Cancer-related molecular mechanisms research
- Alkaline Phosphatase Research Studies
- Immune Response and Inflammation
- Biochemical and Molecular Research
- Biomedical Research and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- Hereditary Neurological Disorders
- Viral Infections and Immunology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Lymphoma Diagnosis and Treatment
Centre National de la Recherche Scientifique
2005-2024
Institut des Neurosciences Paris-Saclay
2015-2024
Université Paris-Saclay
2017-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2024
CEA Paris-Saclay
2020-2024
Retina France
2015-2021
Centre Ophtalmologique d'Imagerie et de Laser
2015-2021
Université Paris-Sud
2015-2019
Centre Hospitalier Universitaire de Bordeaux
2012-2014
Université Paris Cité
2010
Contrasting with fish or amphibian, retinal regeneration from Müller glia is largely limited in mammals. In our quest toward the identification of molecular cues that may boost their stemness potential, we investigated involvement Hippo pathway effector YAP (Yes-associated protein), which upregulated cells following injury. Conditional Yap deletion mouse prevents cell-cycle gene upregulation normally accompanies reactive gliosis upon photoreceptor cell death. We further show that, Xenopus, a...
We evaluated the impact of cytomegalovirus (CMV)–induced immune responses, autoimmune-induced and microbial translocation on activation in 191 human immunodeficiency virus type 1–infected patients from ANRS CO3 Aquitaine Cohort. All enrolled subjects had achieved long-term virological suppression during receipt combination antiretroviral therapy (cART). HLA-DR+/CD38+ expression was 16.8% among CD8+ T cells. Independent age, CD4+ T-cell count, 16S ribosomal DNA load, regulatory positive...
Purpose: During retinal degeneration, Müller glia cells respond to photoreceptor loss by undergoing reactive gliosis, with both detrimental and beneficial effects. Increasing our knowledge of the complex molecular response degeneration is thus essential for development new therapeutic strategies. The purpose this work was identify factors involved in cell death. Methods: Whole transcriptome sequencing performed from wild-type degenerating rd10 mouse retinas at P30. changes mRNA abundance...
The adult frog retina retains a reservoir of active neural stem cells that contribute to continuous eye growth throughout life. We found Yap, downstream effector the Hippo pathway, is specifically expressed in these cells. Yap knock-down leads an accelerated S-phase and abnormal progression DNA replication, phenotype likely mediated by upregulation c-Myc. This associated with increased occurrence damage eventually p53-p21 pathway-mediated cell death. Finally, we identified PKNOX1,...
Recombinant amelogenin gene splice products A+4 and A‐4, implanted in the pulp, induce recruitment, proliferation, differentiation of reparative cells. Our aim was to investigate precocious events occurring pulp 1 d 3 after implantation agarose beads alone or loaded with A‐4. Proliferation cell recruitment towards an odonto/osteogenic phenotype were visualized by detection proliferation nuclear antigen (PCNA) RP59. After A+4, at day 3, cells moderately immunopositive for osteopontin (OP),...
Hippo signalling regulates eye growth during embryogenesis through its effectors YAP and TAZ. Taking advantage of a Yap heterozygous mouse line, we here sought to examine function in adult neural retina, where expression is restricted Müller glia. We first discovered an unexpected temporal dynamic gene compensation. At postnatal stages, Taz upregulation occurs, leading gain function-like phenotype characterised by EGFR potentiation delayed cell-cycle exit retinal progenitors. In contrast,...
Abstract Glaucoma is a multifactorial neurodegenerative disease characterized by the progressive and irreversible degeneration of optic nerve retinal ganglion cells. Despite medical advances aiming at slowing degeneration, around 40% treated glaucomatous patients will undergo vision loss. It thus utmost importance to have better understanding investigate more deeply its early causes. The transcriptional coactivator YAP, an important regulator eye homeostasis, has recently drawn attention in...
The replication of the genomic RNA hepatitis C virus (HCV) positive polarity involves synthesis a intermediate negative by viral RNA-dependent polymerase (NS5B) . In vitro and likely in vivo , NS5B initiates without primers. This de novo mechanism needs specific interactions between elements Cis -acting involved initiation (–) have been identified 3′ non-coding region coding HCV RNA. However, detailed contribution sequences and/or structures (+) has less studied. this report, we an element...
The leukemia inhibitory factor (LIF) receptor comprises the low affinity binding chain gp190 and high converter gp130. ectodomain of is among most complex in hematopoietin family, because it contains two typical cytokine homology domains separated by an immunoglobulin-like (Ig-like) domain. Human murine proteins share 76% homology, but binds human LIF with a much higher affinity, property attributed to Ig-like Using alanine-scanning mutagenesis domain, we mapped site at its carboxyl...
Objectives To unravel the complex relationships between cytomegalovirus-induced-, autoimmune-induced responses, microbial translocation and chronic immune activation (CIA) in successfully treated HIV-infected patients to explore mediating role of alpha-interferon these processes. Design Cross-sectional study nested ANRS CO3 Aquitaine Cohort, a prospective hospital-based cohort HIV-1-infected South-Western France. Methods Patients initiated antiretroviral therapy 2005 2008 were with sustained...
The receptor for the cytokine leukemia inhibitory factor (LIF) associates low affinity binding component gp190 and high converter gp130. Both are members of hematopoietic receptors family characterized by homology (CRH) domain, which consists two barrel-like modules around 100 amino acids each. is among very few this large to contain CRH domains. membrane-distal one (herein called D1) followed an immunoglobulin-like a membrane-proximal domain D2, three type III fibronectin-like repeats. A...
Unlike mammals, some nonmammalian species recruit Müller glia for retinal regeneration after injury. Identifying the underlying mechanisms may help to foresee regenerative medicine strategies. Using a
Abstract Contrasting with fish or amphibian, retinal regeneration from Müller glial cells is largely limited in mammals. In our quest towards the identification of molecular cues that may boost their stemness potential, we investigated involvement Hippo pathway effector YAP, which previously found to be upregulated following injury. We report conditional Yap deletion prevents upregulation cell cycle genes normally accompanies reactive gliosis upon photoreceptor death. This occurs as a...
ABSTRACT Hippo signalling regulates eye growth during embryogenesis through its effectors YAP and TAZ. Taking advantage of a Yap heterozygous mouse line, we here sought to examine function in adult neural retina, where expression is restricted Müller glia. We first discovered an unexpected temporal dynamic gene compensation. At post-natal stages, Taz upregulation occurs, leading gain function-like phenotype characterized by EGFR potentiation delayed cell cycle exit retinal progenitors. In...