A5036731629- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- RNA Research and Splicing
- Hedgehog Signaling Pathway Studies
- Zebrafish Biomedical Research Applications
- Photochromic and Fluorescence Chemistry
- Dermatologic Treatments and Research
- Cellular transport and secretion
- Neuroscience and Neural Engineering
- RNA regulation and disease
- Vascular Tumors and Angiosarcomas
- Cancer and Skin Lesions
- Wnt/β-catenin signaling in development and cancer
- Retinopathy of Prematurity Studies
- Ocular Oncology and Treatments
- RNA modifications and cancer
- Ocular Disorders and Treatments
- Cancer-related molecular mechanisms research
- Microtubule and mitosis dynamics
- Retinal and Macular Surgery
- Retinal Diseases and Treatments
Université Paris-Saclay
2006-2024
Institut des Neurosciences Paris-Saclay
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2024
CEA Paris-Saclay
2020-2024
Université Paris-Sud
1999-2019
Centre Ophtalmologique d'Imagerie et de Laser
2015-2017
Retina France
2015-2017
Doheny Eye Institute
2015
University of California, Los Angeles
2015
Contrasting with fish or amphibian, retinal regeneration from Müller glia is largely limited in mammals. In our quest toward the identification of molecular cues that may boost their stemness potential, we investigated involvement Hippo pathway effector YAP (Yes-associated protein), which upregulated cells following injury. Conditional Yap deletion mouse prevents cell-cycle gene upregulation normally accompanies reactive gliosis upon photoreceptor cell death. We further show that, Xenopus, a...
Sonic hedgehog is involved in eye field separation along the proximodistal axis. We show that Hh signalling continues to be important defining aspects of axis as optic vesicle and cup mature. two other Hedgehog proteins, Banded Cephalic hedgehog,related mouse Indian Desert hedgehog, respectively, are strongly expressed central retinal pigment epithelium but excluded from peripheral surrounding ciliary marginal zone. By contrast, downstream components pathway, Gli2,Gli3 X-Smoothened, this...
Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cycle have not closely studied. In vertebrate retina, Hedgehog's are controversial, with some reports emphasizing increased and others pointing role exit. Here we demonstrate novel for speeding up developing retina by reducing length G1 G2 phases. These fast cycling cells tend exit early. Conversely, retinal progenitors blocked more slowly, longer phases, remain longer. may modulate...
Continuous neurogenesis in the adult nervous system requires a delicate balance between proliferation and differentiation. Although Wnt/β-catenin Hedgehog signalling pathways are thought to share mitogenic function neural stem/progenitor cells, it remains unclear how they interact this process. Adult amphibians produce retinal neurons from pool of stem cells localised ciliary marginal zone (CMZ). Surprisingly, we found that perturbations Wnt result opposite proliferative outcomes CMZ....
Usher syndrome type 1 (USH1) causes combined hearing and sight defects, but how mutations in USH1 genes lead to retinal dystrophy patients remains elusive. The protein complex is associated with calyceal processes, which are microvilli of unknown function surrounding the base photoreceptor outer segment. We show that Xenopus tropicalis, these processes connected outer-segment membrane by links composed protocadherin-15 (USH1F protein). Protocadherin-15 deficiency, obtained a knockdown...
Xath3 encodes a Xenopus neuronal-specific basic helix–loop–helix transcription factor related to the Drosophila proneural atonal. We show here that acts downstream of X-ngnr-1 during neuronal differentiation in neural plate and retina its expression activity are modulated by Notch signaling. activates NeuroD different mechanisms, latter two genes crossactivate each other. In ectoderm, have similar activities, inducing ectopic sensory neurons. Among sensory-specific markers tested, only those...
The retina of fish and amphibian contains genuine neural stem cells located at the most peripheral edge ciliary marginal zone (CMZ). However, their cell-of-origin as well mechanisms that sustain maintenance during development are presently unknown. We identified Hes4 (previously named XHairy2), a gene encoding bHLH-O transcriptional repressor, cell-specific marker Xenopus CMZ is positively regulated by canonical Wnt pathway negatively Hedgehog signaling. found retinogenesis, labels small...
Purpose: During retinal degeneration, Müller glia cells respond to photoreceptor loss by undergoing reactive gliosis, with both detrimental and beneficial effects. Increasing our knowledge of the complex molecular response degeneration is thus essential for development new therapeutic strategies. The purpose this work was identify factors involved in cell death. Methods: Whole transcriptome sequencing performed from wild-type degenerating rd10 mouse retinas at P30. changes mRNA abundance...
Abstract Regenerative abilities are not evenly distributed across the animal kingdom. The underlying modalities also highly variable. Retinal repair can involve mobilization of different cellular sources, including ciliary marginal zone (CMZ) stem cells, retinal pigmented epithelium (RPE), or Müller glia. To investigate whether magnitude damage influences regeneration modality Xenopus retina, we developed a model based on cobalt chloride (CoCl 2 ) intraocular injection, allowing for...
Abstract Background In recent years, considerable knowledge has been gained on the molecular mechanisms underlying retinal cell fate specification. However, hitherto studies focused primarily six major classes (five types of neurons one type glial cell), and paid little attention to specification different neuronal subtypes within same class. particular, machinery governing two most abundant neurotransmitter phenotypes in retina, GABAergic glutamatergic, is largely unknown. spinal cord...
The adult frog retina retains a reservoir of active neural stem cells that contribute to continuous eye growth throughout life. We found Yap, downstream effector the Hippo pathway, is specifically expressed in these cells. Yap knock-down leads an accelerated S-phase and abnormal progression DNA replication, phenotype likely mediated by upregulation c-Myc. This associated with increased occurrence damage eventually p53-p21 pathway-mediated cell death. Finally, we identified PKNOX1,...
Abstract A striking aspect of tissue regeneration is its uneven distribution among different animal classes, both in terms modalities and efficiency. The retina does not escape the rule, exhibiting extraordinary self‐repair properties anamniote species but extremely limited ones mammals. Among cellular sources prone to contribute retinal are Müller glial cells, which teleosts have been known for a decade re‐acquire stem/progenitor state regenerate neurons following injury. As their...
Abstract Vertebrate retinal stem cells, which reside quiescently within the ciliary margin, may offer a possibility for treatment of degenerative retinopathies. The highly proliferative precursor cells in Xenopus eyes are confined to most peripheral region, called marginal zone (CMZ). Although canonical Wnt pathway has been implicated developing retina different species, little is known about its involvement postembryonic retinas. Using green fluorescent protein-based Wnt-responsive...