A5024713064- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- bioluminescence and chemiluminescence research
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
- Receptor Mechanisms and Signaling
- Biofuel production and bioconversion
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Nuclear Structure and Function
- Pancreatic and Hepatic Oncology Research
- Nicotinic Acetylcholine Receptors Study
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Protein Structure and Dynamics
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
University of Copenhagen
2017-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2022
Nano Hydrophobics (United States)
2019
European Molecular Biology Laboratory
2012-2016
Czech Academy of Sciences
2011
Article28 February 2012Open Access Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium Vera van Noort Structural Computational Biology Unit, European Molecular Laboratory, EMBL, Heidelberg, Germany Search for more papers by this author Jan Seebacher Samuel Bader Shabaz Mohammed Biomolecular Mass Spectrometry Proteomics, Bijvoet Center Research Utrecht Institute Pharmaceutical Sciences, University, Utrecht, The Netherlands Ivana Vonkova Matthew J Betts Cell...
Many cellular processes involve the recruitment of proteins to specific membranes, which are decorated with distinctive lipids that act as docking sites. The phosphoinositides form signaling hubs, and we examine mechanisms underlying recruitment. We applied a physiological, quantitative, liposome microarray-based assay measure membrane-binding properties 91 pleckstrin homology (PH) domains, most common phosphoinositide-binding target. 10,514 experiments quantified role in membrane For...
Formation of the immunological synapse between an antigen-presenting cell (APC) and a T leads to signal generation in both cells involved. In cells, lipid raft-associated transmembrane adaptor protein LAT plays central role. Its phosphorylation is crucial step propagation, including calcium response mitogen-activated kinase activation, largely depends on its association with SLP76 protein. Here we report discovery new palmitoylated protein, termed SCIMP. SCIMP expressed B other professional...
The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and characterization to explore properties beyond those individual proteins, we obtained transcriptomics proteomics data, integrated them with computational annotation methods a multitude experiments conducted by biology community. We considerably improved genome characterized transcripts expression thousands genes. furthermore show that composition...
Membrane curvature has recently been recognized as an active regulator of cellular function, with several protein families identified sensors and generators membrane curvature. Amongst them, the inverse Bin/Amphiphysin/Rvs (I-BAR) domain family implicated in sensing generation structures negative e.g. filopodia or dendritic spines. However, to date, quantitative biophysical investigations I-BAR domains have mostly taken place reconstitution. Here, we use fluorescence microscopy...
In the last decade, zebrafish have accompanied mouse as a robust animal model for cancer research. The possibility of screening small-molecule inhibitors in large number embryos makes this particularly valuable. However, dynamic visualization fluorescently labeled tumor cells needs to be complemented by more sensitive, easy, and rapid mode evaluating growth vivo enable high-throughput clinically relevant drugs. study we proposed validated pre-clinical drug discovery utilizing bioluminescence...
Abstract In the course of cancer progression tumor cells undergo morphological changes that lead to increased motility and invasiveness thus promoting formation metastases. This process called epithelial mesenchymal transition (EMT) is triggered by transforming growth factor (TGFβ) but for gaining full invasive potential an interplay between signaling TGFβ Ras GTPases required. proteins possess a lipidated domain mediates association with plasma membrane, which essential biological...
ABSTRACT In the last decade, zebrafish have accompanied mouse as a robust animal model for cancer research. The possibility of screening small-molecule inhibitors in large number embryos makes this particularly valuable. However, dynamic visualization fluorescently labeled tumor cells needs to be complemented by more sensitive, easy, and rapid mode evaluating growth vivo enable high-throughput clinically relevant drugs. study we proposed validated pre-clinical drug discovery utilizing...
Abstract In the course of cancer progression tumor cells undergo morphology changes that lead to increased motility and invasiveness promoting formation metastases. This process called epithelial mesenchymal transition (EMT) is triggered by transforming growth factor (TGFβ) but for gaining full invasive potential an interplay between signaling TGFβ Ras GTPases required. proteins possess lipidated domain mediates association with plasma membrane, which essential biological functions. Type...