A5061654853- Particle Accelerators and Free-Electron Lasers
- Particle accelerators and beam dynamics
- Gyrotron and Vacuum Electronics Research
- Advanced X-ray Imaging Techniques
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Laser-Plasma Interactions and Diagnostics
- Laser Design and Applications
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Nuclear Physics and Applications
- Immune Cell Function and Interaction
- Advanced Surface Polishing Techniques
- CNS Lymphoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ion channel regulation and function
- Autophagy in Disease and Therapy
- Radiation Therapy and Dosimetry
- Photodynamic Therapy Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- Thermal Radiation and Cooling Technologies
- Cellular transport and secretion
Tokushima University
2010-2024
Tohoku University
1956-2022
Nihon University
2000-2012
Kyoto University
2000-2011
Kyoto Pharmaceutical University
2007-2010
Kansai Medical University
2002-2007
Yokohama Rosai Hospital
2007
Center for Rheumatology
2006
Saitama Medical University
2005
Nagasaki Rousai Hospital
2004
Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. Here, we studied the kinetics mechanics of formation in live Jurkat leukemic T cells using proteins fluorescently tagged with variants enhanced GFP (EGFP). Within seconds contacting coverslips coated stimulatory antibodies, developed small, dynamically regulated clusters which were enriched TCR, phosphotyrosine, ZAP-70, LAT, Grb2, Gads, SLP-76, excluded lipid...
Immune complex-mediated inflammation is a common mechanism of various autoimmune diseases. Glomerulonephritis (GN) one these diseases, and the main induction GN has been unclear. We examined contribution Fc receptors in nephrotoxic by establishing analyzing mice deficient receptor gamma chain (FcRgamma). Whereas all wild-type died from severe glomerulonephritis with hypernitremia administration anti-glomerular basement membrane (GBM) antibodies, FcRgamma-deficient survived. Histologically,...
Toll like receptors (TLRs) use Toll–IL-1 receptor (TIR) domain-containing adapters, such as myeloid differentiation primary response gene 88 (MyD88) and TIR adapter inducing IFN-β (TRIF), to induce activation of transcription factors, including NF-κB, MAP kinases, IFN regulatory factors. TLR signaling also leads PI3K, but the molecular mechanism is not understood. Here we have discovered a unique role for B-cell PI3K (BCAP) in TLR-signaling pathway. We find that BCAP has functional...
Grb2 is an adaptor molecule that couples membrane receptors such as the epidermal growth factor receptor (EGFR) to intracellular signaling pathways. To gain insight into trafficking pathways followed by these molecules after activation EGF, we visualized and EGFR fused GFP spectral variants in single live cells. In nonstimulated cells, Grb2-YFP was primarily localized diffusely cytoplasm, whereas EGFR-CFP found on plasma endocytic structures perinuclear area. Within 1 minute of EGF...
BCAP was recently cloned as a binding molecule to phosphoinositide 3-kinase (PI3K). To investigate the role of BCAP, mutant mice deficient in were generated. While BCAP-deficient are viable, they have decreased numbers mature B cells and B1 cell deficiency. The produce lower titers serum immunoglobulin (Ig)M IgG3, mount attenuated responses T cell–independent type II antigen. Upon receptor cross-linking, exhibit reduced Ca2+ mobilization poor proliferative responses. These findings...
T cell antigen receptor (TCR)•CD3 complex is composed of six different subunits: TCRα and TCRβ CD3γ, CD3δ, CD3ε, CD3ζ. Antigen recognition signals are transduced from TCR to the cytoplasm through cytoplasmic domain CD3 chains. To understand downstream signal transduction pathways, we cloned genes encoding proteins capable binding CD3ε with a probe glutathione S-transferase fused region CD3ε. One these clones was found encode topoisomerase IIβ (topoIIβ). The located within N-terminal 12 amino...
Bone remodeling is regulated by local factors that regulate bone-forming osteoblasts and boneresorbing osteoclasts, in addition to hormonal activity. Recent studies have shown reactive oxygen species (ROS) act as an intracellular signal mediator for osteoclast differentiation. However the role of ROS on osteoblast differentiation poorly understood. Here, we investigated impact osteoblastic MC3T3-E1 cells. Osteogenic induction resulted notable enhancement mineralization expression osteogenic...
Quasi-monochromatic photons of energies 1.6-8.7 MeV have been generated by the Compton backscattering laser light on relativistic electrons in TERAS storage ring. The spectra backscattered measured with an anti-Compton spectrometer system. present system has a 155-cm/sup 3/ coaxial-type pure-Ge detector as central and well-type NaI(Tl) scintillator 8-in phi *8-in outer one. With usual anticoincidence technique, clear photopeak were obtained suppression ratio 2-4. maximum energy spread show...
TRIC channels function as monovalent cation-specific that mediate counter ion movements coupled with ryanodine receptor-mediated Ca2+release from intracellular stores in muscle cells. Mammalian tissues differentially contain two channel subtypes: TRIC-A is abundantly expressed excitable cells, whereas TRIC-B ubiquitously throughout tissues. Here, we report the physiological role of mouse perinatal development. TRIC-B-knockout neonates were cyanotic owing to respiratory failure and died...
The nonreceptor protein tyrosine kinase ZAP-70 is a critical enzyme required for successful T lymphocyte activation. After antigenic stimulation, rapidly associates with cell receptor (TCR) subunits. kinetics of its translocation to the surface, properties specific interaction TCRζ chain expressed as chimeric (TTζ and Tζζ), mobility in different intracellular compartments were studied individual live HeLa cells, using Tζζ fused green fluorescent (ZAP-70 GFP Tζζ–GFP, respectively). Time-lapse...
T cell selection is thought to be determined through the interaction between TCR and Ag/MHC. However, contribution of level signal thymic remains unclear. To address this issue, we analyzed male Ag (HY)-specific transgenic (HYTg) mice crossed with CD3 zeta-deficient (zeta KO) (HYTg/zeta KO), which have impaired signaling TCR. In HYTg/zeta KO mice, number thymocytes was comparable that in normal almost all peripheral cells were HY specific, although these positively selected anergic Ag. From...
Generation of quasi-monochromatic photon beams energy variable between 1.7 - 6.5 MeV has been observed by inducing the Compton backscattering laser light quanta on electrons 305 606 at injection straight section 600-MeV ETL storage ring. A Nd-YAG wavelength 1.0641μm used with output power 0.7 1.2 W. The resultant spectra are measured a pure Ge detector, some which presented in present report together description experimental methods. absolute yields being use an 8 inch Ø × long NaI...
Peroxisome proliferator-activated receptors (PPARs) compose a subfamily of nuclear hormone functioning as transcriptional regulators. Originally, the PPARgamma ligand known thiazolidinedione (TZD) was used for treatment diabetic patients. However, recent studies have shown that TZD also has an antitumor effect inhibits cell growth in several types human malignant neoplasms, including leukemia lines. Since pioglitazone is only currently available clinics Japan and role normal hematopoietic...
Kakizaki, F; Shibuya, M; Yamazaki, T; Yamada, Suzuki, H; Homma, IEditor(s): Cannistra, Lauralyn MD; Carlin, Brian Keteyian, Steven J. PhD
Recombinant proteins prepared by genetic engineering are utilized for various purposes in molecular biology such as production of specific antibodies or investigation the mechanism protein—DNA protein-protein interactions (1). There several methods commonly used to produce recombinant bacteria insect cells (2). Expression a protein E. coli fusion with glutathione S-transferase (GST) is one most popular and easiest (3). However, we often encounter serious problems that some rapidly degraded...