Two hundred young adults with common colds were studied during a 10-month period. Virus culture, antigen detection, PCR, and serology paired samples used to identify the infection. Viral etiology was established for 138 of 200 patients (69%). Rhinoviruses detected in 105 patients, coronavirus OC43 or 229E infection 17, influenza A B virus 12, single infections parainfluenza virus, respiratory syncytial adenovirus, enterovirus found 14 patients. Evidence bacterial seven Four had rise...

Background. Childhood community-acquired pneumonia is a common illness, but there have been relatively few comprehensive studies of the viral and bacterial etiology in developed countries. The aim present investigation was to determine hospitalized children by several laboratory methods. Methods. In 3-year prospective study nasopharyngeal aspirate for acute convalescent serum samples serology were taken from 254 with symptoms infection infiltrates compatible chest radiograph. role 17...

Human bocavirus is a newly discovered parvovirus. It has been detected primarily in children with acute lower respiratory tract infection, but its occurrence, clinical profile, and role as causative agent of disease are not clear.We investigated the presence human by quantitative polymerase chain reaction nasopharyngeal aspirate specimens selected serum samples obtained from 259 (median age, 1.6 years) who had hospitalized for expiratory wheezing. The were analyzed 16 viruses reaction, virus...

Simple and safe strategies for the prevention of viral respiratory tract infections (RTIs) are needed.We hypothesized that early prebiotic or probiotic supplementation would reduce risk virus-associated RTIs during first year life in a cohort preterm infants.In this randomized, double-blind, placebo-controlled trial (ClinicalTrials.gov no. NCT00167700), 94 infants (gestational age, ≥32 + 0 ≤36 6 weeks; birth weight, >1500 g) treated at Turku University Hospital, Turku, Finland, were...

The efficacy of antimicrobial treatment in children with acute otitis media remains controversial.In this randomized, double-blind trial, 6 to 35 months age media, diagnosed the use strict criteria, received amoxicillin-clavulanate (161 children) or placebo (158 for 7 days. primary outcome was time failure from first dose until end-of-treatment visit on day 8. definition based overall condition child (including adverse events) and otoscopic signs media.Treatment occurred 18.6% who...

Abstract Suggested citation for this article: Jartti T, Lehtinen P, Vuorinen Österback R, van den Hoogen B, Osterhaus ADME, et al. Respiratory picornaviruses and respiratory syncytial virus as causative agents of expiratory wheezing in children. Emerg Infect Dis [serial on the Internet]. 2004 Jun [date cited]. Available from: http://www.cdc.gov/ncidod/EID/vol10no6/03-0629.htm

Abstract The persistence of rhinovirus and enterovirus RNAs was studied in the nasal secretions children with acute expiratory wheezing (median age: 1.7 years). On admission, 84 samples from 161 (52%) admitted to hospital were positive by reverse transcriptase‐polymerase chain reaction (RT‐PCR), which detects rhino‐ enteroviruses simultaneously. Of samples, 26 (16%) for rhinovirus, 29 (18%) nontypable rhino‐enterovirus. After 2 weeks, 16 these (19%) still positive. Rhinovirus RNA remained 13...

A new respiratory virus, human metapneumovirus, was recently identified. We detected this virus by PCR in ten (8%) of 132 consecutive children admitted to Turku Hospital, Finland, for acute expiratory wheezing (median age 7 months, range 4–25). The mean duration hospital stay 2·5 days (SD 1·6) and symptoms 19 (8). white blood cell count, C-reactive protein, regulated upon activation, normal T-cell-expressed T-cell-secreted (RANTES) concentrations nasal secretion remained low, whereas...

To obtain data on the clinical manifestations of infection, age distribution, and underlying conditions children with influenza severe enough to lead hospital referral, we performed a retrospective study treated at Turku University Hospital (Turku, Finland) in 1980-1999. Influenza A or B antigen was detected nasopharyngeal aspirates 683 15,420 studied. The median 2.0 years (n=544), that 4.2 (n=139) (P<.001). One-fourth had an medical condition. High fever, cough, rhinorrhea were most...

Background. Rhinoviruses are the most common cause of respiratory tract infections, but transmission in families has not been studied using sensitive and specific molecular detection methods. Methods. Children hospitalized for any infection were screened rhinoviruses. Eight with a rhinovirus-positive index child 16 rhinovirus-negative monitored 3 weeks disease symptoms, presence quantity rhinoviruses nasal swab samples determined by quantitative reverse transcription—polymerase chain...

Serum procalcitonin (PCT), C-reactive protein (CRP) and interleukin-6 (IL-6) concentrations were measured in 126 children hospitalized for community-acquired, radiologically confirmed pneumonia to assess whether these host response values could be used distinguish bacterial from viral pneumonia.The samples PCT, CRP IL-6 measurements obtained on admission or the first day of hospitalization. The etiology was studied with an extensive panel methods that detected 6 bacteria 11 viruses.In all,...

Although idiopathic cardiomyopathies are prognostically important and a common indication for cardiac transplantation in all age groups, the incidence distribution of well-defined pediatric population have been poorly characterized. A retrospective study was carried out Finland 1980–1991 to obtain information on epidemiology childhood cardiomyopathies. The medical records patients aged birth 20 years with cardiomyopathy from five university hospitals 16 central covering entire country were...

To determine the usefulness of nasal swabs as a simple method for detection respiratory viruses, we compared and nasopharyngeal aspirates obtained at same time from opposite nostrils 230 children with upper infection. The sensitivity was comparable to that all major viruses except syncytial virus.

ABSTRACT A broad-range bacterial PCR targeting rRNA genes (rDNAs) was used to directly analyze 536 clinical samples obtained from 459 hospitalized patients during a 4-year study period. The molecular diagnosis based on DNA sequencing of the product compared that by culture. bacteriological concordant for 447 (83%) specimens. Broad-range rDNA only method yielded an etiologic 11 (2.4%) patients. Compared culture and assessment, sensitivity combined with 74.2%, specificity between 98.7 99.6%....

Background. Bacteria are found in 50%–90% of cases acute otitis media (AOM) with or without otorrhea, and viruses 20%–49% cases. However, for at least 15% patients AOM, the microbiological etiology is never determined. Our aim was to specify full middle ear infection by using modern methods concomitantly bacterial viral detection. Methods. The subjects were 79 young children having AOM new onset (<48 h) otorrhea through a tympanostomy tube. Middle fluid samples suctioned from sought culture...

Abstract Human bocavirus (HBoV) is a widespread respiratory virus. To improve diagnostic methods, we conducted immunoglobulin (Ig) G and IgM enzyme immunoassays with recombinant virus–like particles of HBoV as antigen. Acute-phase follow-up serum samples from 258 wheezing children single 115 healthy adults in Finland were examined. Our assays had sensitivity 97% specificity 99.5%. Of adults, 96% immunity; none an acute infection. 48 serologically diagnosed infections, 45 viremic 35 virus...

A new human-pathogenic parvovirus, human bocavirus (HBoV), has recently been discovered and associated with respiratory disease in small children. However, many patients have presented low viral DNA loads, suggesting HBoV persistence rendering polymerase chain reaction-based diagnosis problematic. Moreover, nothing is known of immunity. We examined HBoV-specific systemic B cell responses assessed their diagnostic use young children disease.Paired serum samples from 117 acute wheezing,...