A5083271106- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Prostate Cancer Treatment and Research
- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Microscopic Colitis
- Cancer-related gene regulation
- Inflammatory Bowel Disease
- Pancreatic and Hepatic Oncology Research
- Genomics and Chromatin Dynamics
- GaN-based semiconductor devices and materials
- Cancer Genomics and Diagnostics
- Clostridium difficile and Clostridium perfringens research
- Congenital heart defects research
- Immune Response and Inflammation
- Quality and Supply Management
- Pancreatic function and diabetes
- Cancer Mechanisms and Therapy
- Biochemical and Structural Characterization
- Molecular Biology Techniques and Applications
Purdue University West Lafayette
2018-2024
Center for Cancer Research
2021
Jawaharlal Nehru University
2014-2017
Abstract Switch/sucrose-nonfermentable (SWI/SNF) chromatin-remodeling complexes are critical regulators of chromatin dynamics during transcription, DNA replication, and repair. A recently identified SWI/SNF subcomplex termed GLTSCR1/1L-BAF (GBAF; or “noncanonical BAF”, ncBAF) uniquely contains bromodomain-containing protein BRD9 glioma tumor suppressor candidate region 1 (GLTSCR1) its paralog GLTSCR1-like (GLTSCR1L). Recent studies have a unique dependency on GBAF (ncBAF) in synovial sarcoma...
Abstract Polycomb group (PcG) proteins are epigenetic regulators that facilitate both embryonic development and cancer progression. PcG form repressive complexes 1 2 (PRC1 PRC2). PRC2 trimethylates histone H3 lysine 27 (H3K27me3), a mark recognized by the N‐terminal chromodomain (ChD) of CBX subunit canonical PRC1. There five paralogs in humans. CBX2 particular is upregulated variety cancers, particularly advanced prostate cancers. Using inhibitors to understand target highly desirable;...
ARID2 is the most recurrently mutated SWI/SNF complex member in melanoma; however, its tumor-suppressive mechanisms context of chromatin landscape remain to be elucidated. Here, we model deficiency melanoma cells, which results defective PBAF assembly with a concomitant genomic redistribution BAF complex. Upon depletion, subset and shared BAF-PBAF-occupied regions displays diminished accessibility associated gene expression, while BAF-occupied enhancers gain expression genes linked process...
p53 is an important tumor-suppressor protein that mutated in more than 50% of cancers. Strategies for restoring normal function are complicated by the oncogenic properties mutant and have not met with clinical success. To counteract activity, a variety drugs potential to reconvert active wildtype form been developed. However, these associated various negative effects such as cellular toxicity, nonspecific binding other proteins, inability induce response cancer tissue. Here, we report on...
PBRM1 is a subunit of the PBAF chromatin remodeling complex, which mutated in 40-50% clear cell renal carcinoma patients. It thought to largely function as binding but molecular mechanism underlying this activity not fully known. contains six tandem bromodomains are known cooperate nucleosomes acetylated at histone H3 lysine 14 (H3K14ac). Here, we demonstrate that second and fourth from also bind nucleic acids, selectively associating with double stranded RNA elements. Disruption pocket...
Two‐component signal transduction systems (TCS), consisting of a sensor histidine protein kinase and its cognate response regulator, are an important mode environmental sensing in bacteria. Additionally, they have been found to regulate virulence determinants several pathogens. Bacillus anthracis , the causative agent anthrax bioterrorism agent, harbours 41 pairs TCS. However, their role pathogenicity has remained largely unexplored. Here, we show that WalRK B. forms functional TCS which...
Polybromo1 (PBRM1) is a chromatin remodeler subunit highly mutated in cancer, particularly clear cell renal carcinoma. PBRM1 member of the SWI/SNF subcomplex, PBAF (PBRM1-Brg1/Brm-associated factors), and characterized by six tandem bromodomains. Here we establish role for epithelial maintenance through expression genes involved adhesion, metabolism, stress response, apoptosis. In support general response apoptosis, observe that loss results an increase reactive oxygen species generation...
The polybromo, brahma-related gene 1-associated factors (PBAF) chromatin remodeling complex subunit polybromo-1 (PBRM1) contains six bromodomains that recognize and bind acetylated lysine residues on histone tails other nuclear proteins. PBRM1 thus provide a link between epigenetic posttranslational modifications PBAF modulation of accessibility transcription. As putative tumor suppressor in several cancers, protein expression is often abrogated by truncations deletions. However, ∼33%...
Abstract Surface localized microbial enolases’ binding with human plasminogen has been increasingly proven to have an important role in initial infection cycle of several pathogens. Likewise, surface Mycobacterium tuberculosis ( Mtb ) enolase also binds plasminogen, and this interaction may entail crucial consequences for granuloma stability. The current study is the first attempt explore interacting residues from . Beginning structural modeling enolase, pose was predicted using...
Polycomb repressive complexes (PRCs) are a heterogenous collection of dozens, if not hundreds, protein composed various combinations subunits. PRCs transcriptional repressors important for cell-type specificity during development, and as such, commonly mis-regulated in cancer. broadly characterized PRC1 with histone ubiquitin ligase activity, or PRC2 methyltransferase activity; however, the mechanism by which individual PRCs, particularly highly diverse set PRC1s, alter gene expression has...
Two component systems (TCSs) can be envisaged as complex molecular devices that help the bacteria to sense its environment and respond aptly. 41 TCSs are predicted in Bacillus anthracis, a potential bioterrorism agent, of which only four have been studied so far. Thus, intricate signaling network contributed by remains largely unmapped B. anthracis needs comprehensive exploration. In this study, we functionally characterized one such system composed BAS0540 (Response regulator) BAS0541...
GLTSCR1, a protein encoded by the Bicra gene, is defining subunit of SWI/SNF (also called mammalian BAF) chromatin remodeling subcomplex GBAF/ncBAF. To determine role GLTSCR1 during mouse development, we generated germline knockout using CRISPR/Cas9. Mice with homozygous loss were born at Mendelian ratios but small, pale and died within 24 hours after birth. Histology indicated blood-related defects including defective erythroblastic islands irregularly sized red blood cells. Gene expression...
Polybromo1 (PBRM1) is a chromatin remodeler subunit highly mutated in cancer, particularly renal clear cell carcinoma. PBRM1 member of the SWI/SNF subcomplex, PBAF (PBRM1- Brg1/Brm Associated Factors) and characterized by six tandem bromodomains. Here we establish role for epithelial maintenance through expression genes involved adhesion, metabolism, stress response, apoptosis. In support general response apoptosis, observe that loss results an increase reactive oxygen species generation...
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Oncogenic mutations in KRAS are present approximately 95% of patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) and considered the initiating event intraepithelial neoplasia (PanIN) precursor lesions. While it is well established that drive activation oncogenic kinase cascades during oncogenesis, effects signaling on regulation phosphatases this process not fully appreciated. Protein Phosphatase 2A (PP2A) has been implicated suppressing KRAS-driven cellular transformation....
<div>Abstract<p>Switch/sucrose-nonfermentable (SWI/SNF) chromatin-remodeling complexes are critical regulators of chromatin dynamics during transcription, DNA replication, and repair. A recently identified SWI/SNF subcomplex termed GLTSCR1/1L-BAF (GBAF; or “noncanonical BAF”, ncBAF) uniquely contains bromodomain-containing protein BRD9 glioma tumor suppressor candidate region 1 (GLTSCR1) its paralog GLTSCR1-like (GLTSCR1L). Recent studies have a unique dependency on GBAF (ncBAF)...