A5086410760- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Thermal Regulation in Medicine
- Retinoids in leukemia and cellular processes
- Single-cell and spatial transcriptomics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Urological Disorders and Treatments
- Infrared Thermography in Medicine
- Cardiovascular Function and Risk Factors
- Hepatocellular Carcinoma Treatment and Prognosis
- Protein Degradation and Inhibitors
- Lung Cancer Diagnosis and Treatment
- Cardiac Health and Mental Health
- Heart Failure Treatment and Management
- Occupational and environmental lung diseases
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Pleural and Pulmonary Diseases
- Human-Animal Interaction Studies
- Cancer Treatment and Pharmacology
- Synthetic Organic Chemistry Methods
- Pediatric Urology and Nephrology Studies
- Advanced biosensing and bioanalysis techniques
- Urinary Bladder and Prostate Research
Hallands sjukhus Halmstad
2012-2024
Lund University
2019-2024
Skåne University Hospital
2017-2021
Abstract Next‐generation sequencing techniques have revealed that leukemic cells in acute myeloid leukemia often are characterized by a limited number of somatic mutations. These mutations can be the basis for detection follow‐up samples. The aim this study was to identify leukemia‐specific from patients with and use these as markers minimal residual disease. Leukemic normal lymphocytes were simultaneously isolated at diagnosis 17 using fluorescence‐activated cell sorting. Exome identified...
Abstract Introduction Reverse transcriptase quantitative PCR (RT‐qPCR) is considered the method of choice for measurable residual disease (MRD) assessment in NPM1 ‐mutated acute myeloid leukemia (AML). MRD can also be determined with DNA‐based methods offering certain advantages. We here compared (qPCR), droplet digital (ddPCR), and targeted deep sequencing (deep seq) RT‐qPCR. Methods Of 110 follow‐up samples from 30 patients AML were analyzed by qPCR, ddPCR, seq, To select DNA cutoffs bone...
Heart failure (HF) has a high prevalence in an elderly population and leads to substantial hospitalization mortality. The objective of this study was investigate factors that affect mortality population.
We studied clinical outcomes, especially regarding colorectal adenocarcinoma, in patients who underwent ureterosigmoidostomy early childhood between 1944 and 1961.A total of 25 consecutive at a mean age 3.1 years. The most common indication for was bladder exstrophy-epispadias complex. study period ended 2010. Patient files were retrospectively evaluated, personal telephone interviews performed histology reevaluated. One girl died 4 days postoperatively excluded.Of the 24 17 alive 2010 with...
Multicolor flow cytometry (MFC) and real‐time quantitative PCR (RQ‐PCR) are important independent techniques to determine minimal residual disease (MRD) in acute myeloid leukemia (AML). MFC is the standard method, but may be unreliable. Therefore, MFC‐based determination of MRD with an RQ‐PCR‐based approach targeting nucleophosmin 1 ( NPM1 ) type A mutation was set out compare. Since most current RQ‐PCR protocols suffer from clear definitions quantifiability, we sought define quantifiability...
Abstract Background Cell blocks (CBs) are widely used for biomarker analyses such as immunostaining. Although immunohistochemistry on formalin‐fixed paraffin‐embedded tissues is standardized, there multiple preparation methods and fixatives cytology. Our objective was to investigate the effect of different common immunoreactivity pleural effusion CBs with metastatic lung adenocarcinomas. Methods This prospective study included 24 malignant effusions from patients adenocarcinoma. From each...
We studied mutation kinetics in ten relapsing and four non-relapsing patients with acute myeloid leukemia by whole exome sequencing at diagnosis to identify leukemia-specific mutations monitored selected multiple time-points using IBSAFE droplet digital PCR. Five nine could track leukemic clones prior clinical relapse 10/10 the where measurable residual disease was negative multicolor flow cytometry. In patients, load of gradually declined response different therapeutic strategies. Three...
AML with mutated NPM1 occurs in all age groups. Yet, the mutational pattern is not extensively studied very old, which may hamper appropriate risk assessment. Herein we examined 22 cases of NPM1-mutated de novo patients older than 75, a median 84. All diagnostic samples were sequenced aiming for coverage most relevant AML-associated mutations. For comparison younger patients, used already published data on several cohorts. A total 76 mutations including 50 different variants identified 16...