A5021228698- Gut microbiota and health
- Burn Injury Management and Outcomes
- Alcohol Consumption and Health Effects
- Diet and metabolism studies
- Immune Response and Inflammation
- Telomeres, Telomerase, and Senescence
- Cytomegalovirus and herpesvirus research
- Wound Healing and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Diet, Metabolism, and Disease
- Influenza Virus Research Studies
- Pressure Ulcer Prevention and Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Intensive Care Unit Cognitive Disorders
- Liver Disease Diagnosis and Treatment
- Inflammasome and immune disorders
- Pneumonia and Respiratory Infections
- Adenosine and Purinergic Signaling
- Blood groups and transfusion
- Dietary Effects on Health
- Barrier Structure and Function Studies
- Blood properties and coagulation
- Adipose Tissue and Metabolism
- Primate Behavior and Ecology
University of Colorado Anschutz Medical Campus
2019-2024
University of Colorado Denver
2017-2024
VA Eastern Colorado Health Care System
2022-2024
Veterans Health Administration
2024
Johns Hopkins Medicine
2014-2022
Johns Hopkins University
2014-2022
Massachusetts Institute of Technology
2022
St Clements University Higher Education School
2022
Creative Commons
2022
National Institute on Aging
2014
Telomeres are essential in maintaining chromosome integrity and controlling cellular replication. Attrition of telomere length peripheral blood mononuclear cells (PBMCs) with age is well documented from cross-sectional studies. But the actual vivo changes lengths its relationship contributing factors within individuals have not been fully addressed. In present paper, we report a longitudinal analysis PBMCs, lymphocytes monocytes 216 human subjects aged 20-90 years assessed at 0-, 5- 12-year...
Background. Telomeres provide a key mechanism for protecting the integrity of chromosomes and their attrition after cell division during aging are evident in lymphocytes. However, significance telomere shortening age-associated decline immune function is unknown. Methods. We selected 22 HLA-A2–positive healthy older adults who have relatively short or long lengths to compare antibody response against influenza vaccine, CD8+ T-cell an antigen. Results. B cells from individuals with robust...
Background. Sensitive assays are needed for detection of residual human immunodeficiency virus (HIV) in patients with undetectable plasma viral loads to determine whether eradication strategies effective. The gold standard quantitative outgrowth assay (QVOA) underestimates the magnitude reservoir. We sought xenograft leukocytes from HIV type 1 (HIV)–infected into immunocompromised mice would result amplification.
Maintenance of the commensal bacteria that comprise gut microbiome is essential to both and systemic health. Traumatic injury, such as burn, elicits a number changes in gut, including shift composition (dysbiosis), increased leakiness, bacterial translocation into lymphatic system bloodstream. These effects are believed contribute devastating secondary complications following pneumonia, acute respiratory distress syndrome, multi-organ failure, septic shock. Clinical studies demonstrate...
Clinical studies have demonstrated that age 50 years or older is an independent risk factor associated with poor prognosis after burn injury, the second leading cause of traumatic injuries in aged population. While mechanisms driving age-dependent postburn mortality are perplexing, changes intestinal microbiome, may contribute to heightened, dysregulated systemic response seen aging patients. The fecal microbiome from 22 patients admitted a verified center July 2018 February 2019 was...
The unique cellular activity of the telomerase reverse transcriptase ribonucleoprotein (RNP) requires proper assembly protein and RNA components into a functional complex. In ciliate model organism Tetrahymena thermophila , La-domain p65 is required for in vivo telomerase. Single-molecule biochemical studies have shown that promotes efficient with (TERT) protein, part by inducing bend conserved stem IV region (TER). domain architecture consists an N-terminal domain, La-RRM motif, C-terminal...
There are currently > 600 million people over the age of 65 globally and this number is expected to double by year 2050. Alcohol use among population on rise, which concerning as aging associated with increased risk for a chronic illnesses. As most studies investigating effects alcohol have focused young/middle-aged populations, there dearth information regarding consequences in older consumers. In addition, murine ethanol models concentrated exposure very high levels ethanol, while vast...
Alcohol consumption is commonplace in the United States and its prevalence has increased recent years. Excessive alcohol use linked to an risk of infections including pneumococcal pneumonia, mostly commonly caused by Streptococcus pneumoniae . In addition, pneumonia patients with prior often require more intensive treatment longer hospital stays due complications infection. The initial respiratory tract immune response S. includes production pro-inflammatory cytokines chemokines resident...
Abstract Alveolar macrophages (AMs) are tissue-resident cells of the lower airways that perform many homeostatic functions critical for pulmonary health and protection against pathogens. However, little is known about factors shape AMs during healthy aging. In these studies, we sought to characterize age-related changes in AM phenotype, function, responses a physiologic stressor, is, distal injury. Age was associated with wide range cell surface receptor gene expression by AMs, reflecting...
Abstract The Earth’s population is aging and, by 2050, 1 out of 6 people will be 65 or older. Therefore, proper treatment injuries that disproportionately impact advanced age more important. Clinical studies reveal aged ≥65 account for 16.5% all burn injuries, and experience higher morbidity, including neurocognitive decline, mortality we others believe are mediated, in part, heightened intestinal permeability. Herein used our clinically relevant model scald injury young mice to determine...
Abstract Cutaneous burn injury in the elderly is associated with poor clinical outcomes and increased pulmonary-related complications. We others have shown that triggers a cascade of inflammatory mediators which increase gut permeability dysbiosis fecal microbiota this more dramatic aged. Since crosstalk between intestinal microbes lung, termed “gut-lung axis,” impacts immunity homeostasis airway, we hypothesized age may contribute to excessive pulmonary inflammation prognosis after injury....
Abstract One in six people are projected to be 65 years or older by 2050. As the population ages, better treatments for injuries that disproportionately impact aged will needed. Clinical studies show and experience higher rates of morbidity mortality after burn injury, including a greater incidence pulmonary complications when compared younger injured adults, which we others believe is mediated, part, inflammation originating intestines. Herein, use our clinically relevant model scald injury...
The portion of the global population that is over age 65 growing rapidly and this presents a number clinical complications, as aged at higher risk for various diseases, including infection. For example, advanced factor heightened morbidity mortality following infection with Streptococcus pneumoniae . This increased vulnerability due, least in part, to age-related dysregulation immune response, phenomenon termed immunosenescence. However, our understanding mechanisms influencing...
Simian immunodeficiency virus (SIV) infection of macaques recapitulates many aspects HIV pathogenesis and is similarly affected by both genetic environmental factors. Psychosocial stress associated with immune system dysregulation worse clinical outcomes in people HIV. This study assessed the impact single housing, as a model psychosocial stress, on innate responses pigtailed ( Macaca nemestrina ) during acute SIV infection.A retrospective analysis 2- to si6-year-old male was performed...
Abstract Introduction The elderly have a heightened basal state of inflammation, known as “inflamm-aging,” which predisposes them to aberrant immune responses and may reduce their ability withstand burn trauma. We others believe that combination factors released from the site in aged host trigger gut become more permeable and, along with shifts fecal microbiota, sets stage for excessive systemic pulmonary inflammatory poor prognosis after injury. Methods used our well-established, clinically...
Platelet decline is a feature of many acute viral infections, including cytomegalovirus (CMV) infection in humans and mice. sequestration association with other cells, endothelium circulating leukocytes, can contribute to this influence the immune response pathogenesis infection. We sought determine if platelet-endothelial associations (PEAs) platelet during murine CMV (mCMV) infection, these affect load production. Male BALB/c mice were infected mCMV (Smith strain), euthanized at timepoints...