A5027631897- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Machine Learning in Bioinformatics
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Signaling Pathways in Disease
- Silk-based biomaterials and applications
- Viral Infections and Immunology Research
University of California, San Diego
2020-2021
University of North Carolina at Chapel Hill
2015-2018
Significance Protein and mRNA expression are in most cases poorly correlated, which suggests that the posttranscriptional regulatory program of a cell is an important component gene expression. This network still understood, including how RNA structure quantitatively contributes to translational control. We present here series structural functional experiments together allow us derive quantitative, structure-dependent model translation accurately predicts efficiency reporter assays primary...
ADVERTISEMENT RETURN TO ISSUEViewpointNEXTMessenger RNA Structure Regulates Translation Initiation: A Mechanism Exploited from Bacteria to HumansAnthony M. Mustoe*Anthony MustoeDepartment of Chemistry, University North Carolina at Chapel Hill, 27599, United States*E-mail: [email protected]More by Anthony Mustoehttp://orcid.org/0000-0001-9346-1559, Meredith CorleyMeredith CorleyDepartment Biology, StatesMore Corley, Alain LaederachAlain LaederachDepartment Laederach, and Kevin Weeks*Kevin...
Ribonucleic acid (RNA) secondary structure prediction continues to be a significant challenge, in particular when attempting model sequences with less rigidly defined structures, such as messenger and non-coding RNAs. Crucial interpreting RNA structures they pertain individual phenotypes is the ability detect RNAs large structural disparities caused by single nucleotide variant (SNV) or riboSNitches. A recently published human genome-wide parallel analysis of (PARS) study identified number...
Abstract RNA-based medicines and RNA-targeting drugs are emerging as promising new approaches for treating disease. Optimizing these therapeutics by naive experimental screening is a time-consuming expensive process, while rational design requires an accurate understanding of the structure function RNA. To address this challenge, we present ATOM-1, first RNA foundation model trained on chemical mapping data, enabled data collection strategies purposely developed machine learning training....
Abstract This unit describes the selection of aptamers from a pool single‐stranded RNA by binding to protein target. Aptamers generated this experiment can potentially act as function inhibitors, and may find applications therapeutic or diagnostic reagents. A dsDNA is used generate an ssRNA pool, which mixed with Bound complexes are separated unbound reagents filtration, RNA:protein amplified combination reverse transcription, PCR, in vitro transcription. Curr. Protoc. Nucleic Acid Chem ....
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) structure probing techniques characterize the secondary of RNA molecules, which influence their functions and interactions. A variation SHAPE, footprinting SHAPE (fSHAPE), probes in presence absence protein to identify bases that hydrogen-bond with protein. or fSHAPE coupled enhanced crosslinking immunoprecipitation (SHAPE-eCLIP fSHAPE-eCLIP) pulls down RNAs bound any interest returns interaction information, respectively....