A5029664707- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Protease and Inhibitor Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer-related gene regulation
- Transportation and Mobility Innovations
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Carbohydrate Chemistry and Synthesis
- Digital Economy and Work Transformation
- Genetics and Neurodevelopmental Disorders
- Polyamine Metabolism and Applications
- Metabolism and Genetic Disorders
- Hemoglobin structure and function
- Enzyme Structure and Function
- Silk-based biomaterials and applications
- Signaling Pathways in Disease
University of North Carolina at Chapel Hill
1991-2018
University of Florida
2009-2012
Significance Protein and mRNA expression are in most cases poorly correlated, which suggests that the posttranscriptional regulatory program of a cell is an important component gene expression. This network still understood, including how RNA structure quantitatively contributes to translational control. We present here series structural functional experiments together allow us derive quantitative, structure-dependent model translation accurately predicts efficiency reporter assays primary...
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA Phe ), 3# adjacent to the anticodon in Eucarya Archaea.In Saccharomyces cerevisiae, formation yW requires five enzymes acting a strictly sequential order: Trm5, Tyw1, Tyw2, Tyw3, Tyw4.Archaea contain wyosine derivatives, but their diversity is greater than eukaryotes corresponding biosynthesis pathways still unknown.To identify these pathways, we analyzed...
Folding to a well-defined conformation is essential for the function of structured ribonucleic acids (RNAs) like ribosome and tRNA. Structured elements in untranslated regions (UTRs) specific messenger RNAs (mRNAs) are known control expression. The importance unstructured adopting multiple conformations, however, still poorly understood. High-resolution SHAPE-directed Boltzmann suboptimal sampling Homo sapiens Retinoblastoma 1 ( RB1 ) 5′ UTR yields three distinct conformations compatible...
The presence of the 7-deazaguanosine derivative archaeosine (G<sup>+</sup>) at position 15 in tRNA is one diagnostic molecular characteristics Archaea. biosynthesis this modified nucleoside especially complex, involving initial production 7-cyano-7-deazaguanine (preQ<sub>0</sub>), an advanced precursor that produced a tRNA-independent portion biosynthesis, followed by its insertion into enzyme tRNA-guanine transglycosylase (arcTGT), which replaces target guanine base yielding...
The biosynthesis of GTP derived metabolites such as tetrahydrofolate (THF), biopterin (BH(4)), and the modified tRNA nucleosides queuosine (Q) archaeosine (G(+)) relies on several enzymes Tunnel-fold superfamily. A subset these proteins includes 6-pyruvoyltetrahydropterin (PTPS-II), PTPS-III, PTPS-I homologues, all members COG0720 family that have been previously shown to transform 7,8-dihydroneopterin triphosphate (H(2)NTP) into different products. PTPS-II catalyzes formation in BH(4)...
With the availability of a genome sequence and increasingly sophisticated genetic tools, Haloferax volcanii is becoming model for both Archaea halophiles. In order H. to reach status equivalent Escherichia coli, Bacillus subtilis , or Saccharomyces cerevisiae gene knockout collection needs be constructed in identify archaeal essential set enable systematic phenotype screens. A streamlined gene-deletion protocol adapted potential automation was implemented used generate 22 deletion strains...
C-1 carriers are essential cofactors in all domains of life, and Archaea, these can be derivatives tetrahydromethanopterin (H(4)-MPT) or tetrahydrofolate (H(4)-folate). Their synthesis requires 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP) as the precursor, but nature pathways that lead to its formation were unknown until recent discovery GTP cyclohydrolase IB/MptA family catalyzes first step, conversion dihydroneopterin 2',3'-cyclic phosphate 7,8-dihydroneopterin triphosphate [El...
In part due to the existence of simple methods for its cultivation and genetic manipulation, Haloferax volcanii is a major archaeal model organism. It only archaeon which whole set post-transcriptionally modified tRNAs has been sequenced, allowing an in silico prediction all RNA modification genes present One approach check these predictions experimentally via construction targeted gene deletion mutants. Toward this goal, integrative "Gateway vector" that allows H. uracil auxotrophs was...