A5032836610- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- CRISPR and Genetic Engineering
- Genetic factors in colorectal cancer
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Single-cell and spatial transcriptomics
Roche (Switzerland)
2023
University of Twente
2018-2022
University Medical Center Utrecht
2018-2022
Oncode Institute
2018-2022
Utrecht University
2018-2022
Abstract Predicting the toxicity of cancer immunotherapies preclinically is challenging because models tumours and healthy organs do not typically fully recapitulate expression relevant human antigens. Here we show that patient-derived intestinal organoids tumouroids supplemented with immune cells can be used to study on-target off-tumour toxicities T-cell-engaging bispecific antibodies (TCBs), capture clinical predicted by conventional tissue-based as well inter-patient variabilities in TCB...
Abstract Central to tumor evolution is the generation of genetic diversity. However, extent and patterns by which de novo karyotype alterations emerge propagate within human tumors are not well understood, especially at single-cell resolution. Here, we present 3D Live-Seq—a protocol that integrates live-cell imaging organoid outgrowth whole-genome sequencing each imaged cell reconstruct evolving karyotypes across consecutive generations. Using patient-derived colorectal cancer organoids...
CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation DNA double-strand breaks (DSBs) to stimulate homology-directed repair (HDR) or non-homologous end joining (NHEJ)-mediated integration desired knock-in. A major downside associated with DSB-mediated is required clonal selection and expansion candidate verify genomic integrity targeted...