A5089508461- Hippo pathway signaling and YAP/TAZ
- Prostate Cancer Treatment and Research
- Genital Health and Disease
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Pancreatic and Hepatic Oncology Research
- Freezing and Crystallization Processes
- Genomic variations and chromosomal abnormalities
- Fibroblast Growth Factor Research
- Immune cells in cancer
- Chromosomal and Genetic Variations
- Irrigation Practices and Water Management
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Helicobacter pylori-related gastroenterology studies
- Animal Genetics and Reproduction
Oncode Institute
2020-2024
University Medical Center Utrecht
2020-2024
Utrecht University
2020-2022
Background Poor prognosis in colon cancer is associated with a high content of cancer-associated fibroblasts (CAFs) and an immunosuppressive tumor microenvironment. The relationship between these two features incompletely understood. Here, we aimed to generate model system for studying the interaction cells CAFs their effect on immune-related cytokines T cell proliferation. Methods were isolated from liver metastases immortalized prolong lifespan improve robustness reproducibility....
Survival rates of cancer patients vary widely within and between malignancies. While genetic aberrations are at the root all cancers, individual genomic features cannot explain these distinct disease outcomes. In contrast, intra-tumour heterogeneity (ITH) has potential to elucidate pan-cancer survival biology that drives prognosis. Unfortunately, a comprehensive effective framework measure ITH across cancers is missing. Here, we introduce scalable chromosomal copy number (CNH) predicts...
Abstract Micrometastases of colorectal cancer can remain dormant for years prior to the formation actively growing, clinically detectable lesions (i.e., colonization). A better understanding this step in metastatic cascade could help improve metastasis prevention and treatment. Here we analyzed liver specimens patients with monitored real-time mouse livers using intravital microscopy reveal that micrometastatic are devoid stem cells (CSC). However, grow into overt metastases demonstrated...
Abstract Mismatch repair (MMR)-deficient cancer evolves through the stepwise erosion of coding homopolymers in target genes. Curiously, MMR genes MutS homolog 6 ( MSH6) and 3 MSH3 ) also contain homopolymers, these are frequent mutational targets MMR-deficient cancers. The impact incremental mutations on evolution is unknown. Here we show that microsatellite instability modulates DNA by toggling hypermutable mononucleotide homopolymer runs MSH6 stochastic frameshift switching. Spontaneous...
CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation DNA double-strand breaks (DSBs) to stimulate homology-directed repair (HDR) or non-homologous end joining (NHEJ)-mediated integration desired knock-in. A major downside associated with DSB-mediated is required clonal selection and expansion candidate verify genomic integrity targeted...
Intestinal tuft cells are epithelial sentinels that trigger host defense upon detection of parasite-derived compounds. While representing interesting targets for immunomodulatory therapies in inflammation-driven intestinal diseases, their detailed functioning is poorly understood. Although two distinct cell types have been described, we reveal common intermediary transcriptomes among mouse and human. Tuft cell-specific reporter knock-ins organoids show the sequentially expressed...
<div>Abstract<p>Micrometastases of colorectal cancer can remain dormant for years prior to the formation actively growing, clinically detectable lesions (i.e., colonization). A better understanding this step in metastatic cascade could help improve metastasis prevention and treatment. Here we analyzed liver specimens patients with monitored real-time mouse livers using intravital microscopy reveal that micrometastatic are devoid stem cells (CSC). However, grow into overt...
<div>Abstract<p>Micrometastases of colorectal cancer can remain dormant for years prior to the formation actively growing, clinically detectable lesions (i.e., colonization). A better understanding this step in metastatic cascade could help improve metastasis prevention and treatment. Here we analyzed liver specimens patients with monitored real-time mouse livers using intravital microscopy reveal that micrometastatic are devoid stem cells (CSC). However, grow into overt...
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage
Supplementary Data from Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage