A5010155349- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Neural dynamics and brain function
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- EEG and Brain-Computer Interfaces
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- Biochemical and Structural Characterization
- VLSI and FPGA Design Techniques
- Low-power high-performance VLSI design
- Neuroscience and Neural Engineering
- 3D Modeling in Geospatial Applications
- Alzheimer's disease research and treatments
- Manufacturing Process and Optimization
- Antibiotics Pharmacokinetics and Efficacy
- Vibrio bacteria research studies
- Optimal Experimental Design Methods
- Machine Learning in Bioinformatics
- graph theory and CDMA systems
- Bayesian Methods and Mixture Models
- Statistical Methods and Inference
- Product Development and Customization
- Neuroscience and Neuropharmacology Research
Sapienza University of Rome
2022-2023
University of Padua
1981-2016
University of Verona
1997-2000
University of Leicester
1994
University of Sassari
1979
University of Genoa
1974-1978
A total of 8 Pseudomonas aeruginosa isolates was collected from 7 different patients in wards the University Hospital Verona, Italy, February 1997 to 1998. The high level resistance carbapenems (imipenem minimum inhibitory concentration always >128 µg/mL) and other broad-spectrum β-lactams rate imipenem hydrolysis its inhibition by ethylenediamine-tetra-acetic acid were all suggestive production a carbapenem-hydrolyzing metallo-β-lactamase. specific DNA probe derived recently cloned blaVIM-1...
Early diagnosis of Alzheimer's disease (AD) is a very challenging problem and has been attempted through data-driven methods in recent years. However, considering the inherent complexity decoding higher cognitive functions from spontaneous neuronal signals, these benefit incorporation multimodal data. This work proposes an ensembled machine learning model with explainability (EXML) to detect subtle patterns cortical hippocampal local field potential signals (LFPs) that can be considered as...
SUMMARY: In Streptococcus faecium inhibition by both benzylpenicillin and cefotaxime of cells growing at maximal reduced rates was associated with saturation different penicillin-binding proteins. Cells were not inhibited concentrations that saturated all proteins except protein 5, but did stop when this saturated.
The pH-conditional morphology mutant of Klebsiella pneumoniae strain MirM7 grows as cocci at pH 7 and rods 5.8. has a high-level mecillinam resistance (50% lethal dose greater than 200 micrograms/ml) in both forms. When broth cultures the rod-shaped were grown with 0.7 microgram per ml, cells assumed round shape continued to divided higher rate untreated control. A revertant (MirA12), when treated same antibiotic concentration, changed coccal stopped dividing. penicillin-binding proteins...
Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and normal rods 5.8. We studied the rates peptidoglycan synthesis cocci growing values 5.8, respectively. It was found that exponentially produced reduced amount per cell, compared with rods. Moreover, shift to permissive (5.8) caused an increase in rate synthesis, whereas reverse determined twofold reduction [ 3 H]diaminopimelic acid incorporation. During...
SUMMARY: The properties of a conditional mutant Klebsiella pneumoniae (Mir M7) are described. has pH-dependent disturbance morphology and temperature-dependent division. At pH 7 the grows as cocci at 41 °C (restrictive temperature) division is inhibited. acid restrictive temperature, filaments formed. permissive polymorphous obtained. °C, giant cells inhibition phenotypically reverted by 0·05 m-Mg2+, 0·1 m-Na+ or 0·4 m-sucrose. Seventeen revertants were isolated having exactly same...
Klebsiella pneumoniae Mir M7 is a spontaneous parentless morphology mutant which grows as cocci at pH 7 and rods 5.8. This strain has been characterized defective in lateral wall formation (at pH7). Data suggest that the cell mainly made up of poles (G. Satta, R. Fontana, P. Canepari, G. Botta, J. Bacteriol. 137:727--734, 1979). In this work isolation biochemical properties peptidoglycan both nonconditional rod-shaped revertant (strain A12) are described. The (both cocci) A12 strains carried...
The effects of mecillinam on the growth rods pH-conditional morphology mutant MirM7 was studied. It has been found that causes, coincident with transition to coccal shape, a balanced rise in rate viable count increase and macromolecular synthesis which lasts either until cells enter stationary phase or indefinitely, case continuously diluted cultures. When antibiotic is removed from have already become coccoid, continue grow at faster they resume rod shape. No change per-cell protein seen...
Klebsiella pneumoniae MirM7 is a wild-type strain which grows as cocci at pH 7 and above rods 6.5 below. Cultures of this an auxotrophic derivative, MirM7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. Lysates always contained two phages, FR2 AP3, most often high titers. AP3 plated with the same efficiency on both MirM7b K59 (another K. sensitive AP3) lysogenized 45 54% K59-infected cells, respectively. These findings raise possibility...
Two hundred nine temperature-sensitive growth or division (or both) mutants of Streptococcus faecium ATCC 9790 were isolated. These strains examined for timing the block in cell cycle. About 42% isolates blocked at terminal stages division. A second large group appeared to be various septation. Only five a stage before completion chromosome replication. Thirty lysed after one more doublings nonpermissive temperature.
Recent improvements in microelectrodes technology have enabled neuroscientists to record electrophysiological signals from hundreds of neurons and simultaneously a large number channels. However, several environmental factors may introduce noise artefacts affect proper interpretation recordings. Thus, the development appropriate signal acquisition processing platforms dealing with data sets real-time represents current fundamental challenge. In present work, we an easily-expandable Lab VIEW...
The regrowth of 27 temperature-sensitive division mutants Streptococcus faecium ATCC 9790 was examined after various periods incubation at the nonpermissive temperature. Several blocked stages septum formation or daughter-cell separation divided in a partially completely synchronous way short All four lytic early cell cycle normal rate brief lag.
FCE 22101 is a new penem characterized by broad spectrum of activity which includes against methicillin-resistant strains Staphylococcus aureus. The interaction with penicillin-binding protein 2a S. aureus strain has been investigated in the present study. In competition experiments, showed very low affinity for this protein, and concentration more than 4 times MIC was required saturation. When classical competitive procedure modified increasing time incubation either membranes or growing...
The characteristics of three spontaneous mutants Streptococcus faecium PS which overproduce the penicillin-binding protein (PBP) 5 and are resistant to penicillin were investigated compared with those parent. grew a little more slowly than parent, had smaller cellular size, did not grow as filaments in presence minimal inhibitory concentration (MIC), significantly prone autolysis. relationship between overproduction PBP is discussed.
The quantitative study of cross-frequency coupling (CFC) is a relevant issue in neuroscience. In local field potentials (LFPs), measured either the cortex or hippocampus, how γ-oscillation amplitude modulated by changes θ-rhythms-phase thought to be important memory formation. Several methods were proposed quantify CFC, but reported evidence suggests that experimental parameters affect results. Therefore, simulation tool support determination minimal requirements for CFC estimation order...