A5002180270- Cancer Cells and Metastasis
- Diet and metabolism studies
- Nutrition and Health in Aging
- 3D Printing in Biomedical Research
- Muscle metabolism and nutrition
- Cancer, Hypoxia, and Metabolism
- Cancer survivorship and care
- Medical Imaging Techniques and Applications
- Chemical Reactions and Isotopes
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Muscle Physiology and Disorders
- Biosimilars and Bioanalytical Methods
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Dietary Effects on Health
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- Science, Research, and Medicine
University Medical Center Utrecht
2020-2024
University of Arizona Cancer Center
2024
Translational Research in Oncology
2024
Utrecht University
2020-2021
Background Poor prognosis in colon cancer is associated with a high content of cancer-associated fibroblasts (CAFs) and an immunosuppressive tumor microenvironment. The relationship between these two features incompletely understood. Here, we aimed to generate model system for studying the interaction cells CAFs their effect on immune-related cytokines T cell proliferation. Methods were isolated from liver metastases immortalized prolong lifespan improve robustness reproducibility....
Colorectal cancer metastasizes predominantly to the liver but also lungs and peritoneum. The presence of extra-hepatic metastases limits curative (surgical) treatment options is associated with very poor survival. mechanisms governing multi-organ metastasis formation are incompletely understood. Here, we tested hypothesis that site tumor growth influences formation. To this end, implanted murine colon organoids into primary (i.e., caecum) liver) in immunocompetent mice. organoid-initiated...
The liver has a complex and hierarchical segmental organization of arteries, portal veins, hepatic veins lymphatic vessels. In-depth imaging vasculature malignancies could improve knowledge on tumor micro-environment, local growth, invasion, as well metastasis. Non-invasive techniques such computed tomography (CT), magnetic resonance (MRI) positron-emission transmission (PET) are routine for clinical imaging, but show inadequate resolution at cellular subcellular level. In recent years,...
Abstract Introduction The pharmaceutical industry has mostly relied on 2D cancer cell lines and 3D spheroids for in vitro testing, but poor correlation of pre-clinical clinical outcomes driven the development more predictive models. Patient-derived organoids (PDOs) have emerged as representative avatars tumor biology, allowing generation biobanks covering a large variety indications genetic backgrounds. Here, we assess robustness our drug screening platform by testing reproducibility...
Abstract Introduction The development of oncology drugs has a persistently high attrition rate greater than 95%, requiring the and implementation novel models technologies aimed at better predictive abilities during preclinical phase. Among these, organoid technologies, which involve propagating tumor stem cells in near-native state, have proven to markedly advance drug testing with enhanced precision. Our previously developed assay-ready technology paved way for large panel screening,...
Abstract Background Physical activity is associated with a lower risk of colorectal cancer (CRC) and CRC‐specific mortality. However, evidence for causal relationship between physical disease progression lacking. Here, we have used CRC organoids to create novel mouse model spontaneous metastasis formation multiple organs. We this assess the influence voluntary exercise on progression. Methods Collagen‐embedded murine tumour were transplanted into livers immunocompetent C57Bl/6 mice using...
Abstract Background: Physical activity (PA) is associated with a lower risk of colorectal cancer (CRC) initiation and death from CRC. However, evidence for causal link between PA disease progression lacking. This study assesses the effect voluntary exercise on metastases formation in novel pre-clinical mouse model late-stage metastatic Methods: Murine tumor organoids were transplanted into livers immunocompetent C57Bl/6 mice using microsurgery. Animals fed ad libitum. Voluntary tumor-bearing...
Abstract Introduction Patient-derived organoids (PDOs) have emerged over the last decade as representative in vitro replicas of tumor biology, bringing increased accuracy predicting clinical outcomes based on pre-clinical results. Large and deeply characterized organoid biobanks, covering broad varieties indications genetic backgrounds, been generated that allow large scale (combination) drug testing, reliable biomarker predictions, improved repurposing patient stratification studies....
Abstract Introduction: Radiotherapy (RT) is widely used for various cancer types, however, radiotoxicity in healthy neighbouring tissues remains challenging. To optimize effectiveness of current therapeutic regimens, there a clinical need to enhance radiotherapy-mediated killing by radiosensitizing agents. Radiosensitizers show great potential accelerating DNA damage, production free radicals and disturbing cell cycle processes induction cytostatic -toxic effects, but pre-clinical drug...
// Liza A. Wijler 1 , * Francina J. Dijk 2 Hanil Quirindongo Danielle A.E. Raats Bram Dorresteijn Matthew J.W. Furber Anne M. May 3 Onno Kranenburg 4 and Miriam van Laboratory of Translational Oncology, Division Imaging Cancer, University Medical Centre Utrecht, 3584 CX, The Netherlands Danone Nutricia Research, CT, Department Epidemiology, Julius Center for Health Sciences Primary Care, CG, Utrecht Platform Organoid Technology, University, CS, Shared first author Correspondence to: Dijk,...
Skeletal muscle wasting and fatigue are commonly observed in cancer patients receiving chemotherapy associated with reduced treatment outcome quality of life. Nutritional support may mitigate these side effects, but potential interference efficacy could be concern. Here, we investigated the effects a ω-3-PUFA (EPA DHA), leucine-enriched, high protein (100% whey), additional vitamin D prebiotic fibers "Specific Composition" (SNC) on state-of-the-art tumor organoid models cells studied...