Earlier we reported induction of neurotoxicity and neurodegeneration by tryptophan metabolites that link the metabolic alterations to Alzheimer’s disease (AD). Tryptophan is a product Shikimate pathway (SP). Human cells lack SP, which found in human gut bacteria exclusively using SP produce aromatic amino acids (AAA). This study first attempt toward gene-targeted analysis microbiota AD fecal samples. The oligonucleotide primers newly-designed for this work target SP-AAA environmental...

Human gut bacterial Na(+)-transporting NADH:ubiquinone reductase (NQR) sequence is associated with Alzheimer disease (AD). Here, disease-associated (ADAS) further characterized in cultured spore-forming Clostridium sp. Tryptophan and NQR substrate ubiquinone have common precursor chorismate microbial shikimate pathway. Tryptophan-derived tryptamine presents human diet microbiome. Tryptamine inhibits tryptophanyl-tRNA synthetase (TrpRS) consequent neurodegeneration cell animal models....

Neurodegeneration is induced by tryptamine, a human diet constituent, which easily crosses the blood/brain barrier. Tryptamine neurotoxicity, caused tryptophanyl-tRNA synthetase (TrpRS) inhibition and downregulation leads to deficiency synthesis of aberrant proteins. We identified axonal defects in hippocampus tryptamine- treated mice similar those observed brain patients with Alzheimer's disease, multiple sclerosis epilepsy using anti-TrpRS site-directed antibodies. The are characterized...

Upregulation of group IIA phospholipase A(2) (sPLA(2)-IIA) correlates with prostate tumor progression suggesting prooncogenic properties this protein. Here, we report data on expression three different sPLA(2) isozymes (groups IIA, V, and X) in normal (PrEC) malignant (DU-145, PC-3, LNCaP) human cell lines. All studied lines constitutively expressed sPLA(2)-X, whereas sPLA(2)-V transcripts were identified only cells. In contrast, no sPLA(2)-IIA was found PrEC DU-145 cells, but it by...

Tryptophanyl-tRNA synthetase (TrpRS) catalyzes tryptophanyl-tRNAtrp formation. At concentrations exceeding tryptophan, tryptamine inhibits TrpRS. This leads in tryptophanyl-tRNA deficiency and synthesis of aberrant proteins. Tryptamine presents food crosses blood-brain barrier. The purpose this study is to test the hypothesis that tryptamine-induced changes cell animal models correlate with Alzheimer's disease (AD) manifestations. prevented growth human neuroblastoma. Epithelioids recovered...

Transgenic mice used for Alzheimer's disease (AD) preclinical experiments do not recapitulate the human disease. In our models, dietary tryptophan metabolite tryptamine produced by gut microbiome induces tryptophanyl-tRNA synthetase (TrpRS) deficiency with consequent neurodegeneration in cells and mice. Dietary supplements, antibiotics certain drugs increase content vivo. TrpRS catalyzes attachment to tRNAtrp at initial step of protein biosynthesis. Tryptamine that easily crosses blood-brain...

Objectives: Pancreatic cancer is one of most deadly because its aggressive growth and high metastatic ability that correlates with intratumoral hypoxia. Earlier diagnosis prognosis marker pancreatic not yet available. In colorectal cancer, protein biosynthesis enzyme, tryptophanyl-tRNA synthetase (TrpRS), up-regulated in good-prognosis tumors down-regulated poor-prognosis tumors. Tryptophanyl-tRNA status unknown. To correlate hypoxia TrpRS as a possible prognostic marker, we examined mRNA...

The author discussed recently the possible molecular mechanisms that cause COVID-19 disease symptoms. Here analysis of recent experimental data supports hypothesis production gut microbial tryptamine can be induced by SARS-CoV-2 fecal viral activity due to selective pressure or positive selection tryptamine-producing microorganisms. In this report, suggests mechanism bases on abilities affect nucleic acid. other words, microorganisms producing are more resistant than no tryptamine. Earlier...

Tryptophanyl-tRNA synthetase (TrpRS) expression alters in colorectal (CRC), pancreatic (PC), and cervical (CC) cancers. Here, phosphorylation of unfolded TrpRS its fragments is stimulated by human cancer sera (CS; n = 13) serum rabbit tumor induced Rous sarcoma virus, unaffected donor (NS; 11/15) abolished alkaline phosphatase. At 20 years follow-up, serum-inducible found before healthy donors (3/15) diagnosed with PC, CRC, or leukemia. I have examined a specificity found, surprisingly, that...

Background: COVID-19 can be related to any diseases caused by microbial infection(s) because 1) co-infection with COVID-19-related virus and other microorganism(s) 2) metabolites produced microorganisms such as bacteria, fungi, protozoan involved in necrotizing pneumonia medical conditions observed COVID-19. Objective: By way of illustration, the metabolite aromatic amino acid tryptophan, a biogenic amine tryptamine inducing neurodegeneration cell animal models, also induces necrosis....

Bovine Trp-tRNA synthetase is a dimer with subunit molecular mass of 60 kDa (p60) which catalyzes ATP-dependent formation tryptophanyl-tRNA. Evidence presented that whose homogeneity had been proven by SDS/PAGE and silver staining the gel autophosphorylated in vitro. Anti-(Trp-tRNA synthetase) antibodies, specificity was verified using combination different approaches, were able to effectively inhibit immunoprecipitate Trp-tRNA-synthetase-associated kinase activity. The two-dimensional...

ИММУНОМОРФОЛОГИЧЕСКОЕ И БИОХИМИЧЕСКОЕ ИЗУЧЕНИЕ ВНУТРИКЛЕТОЧНОЙ ЛОКАЛИЗАЦИИ ΤРИПТОФАНИЛ-тРНК-СИНТЕТАЗЫ ВЫСШИХ ОРГАНИЗМОВК. Л. Палей, В. Н. Баранов, Η

SV40-transfonned Chinese hamster CO631 cells pretreated with N -methyl- N' -nitro- -nitrosoguanidine (MNNG) display SV40 DNA amplification. This study shows that following MNNG treatment elevated T antigen synthesis and a 4.5-fold reduction in the extent of its phosphorylation occurred both pulselabeled steady-state-labeled cells. The decrease was found to be inversely related carcinogen concentration, i.e. an augmented concentration brought about gradual Although majority sites on derived...