A5049946105- Historical, Literary, and Cultural Studies
- Synthetic Organic Chemistry Methods
- Medieval European History and Architecture
- Historical and Archaeological Studies
- Chemical Synthesis and Reactions
- Inorganic and Organometallic Chemistry
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Metabolism and Genetic Disorders
- German Literature and Culture Studies
- Enzyme Structure and Function
- Linguistics and language evolution
- Mitochondrial Function and Pathology
- Sociology and Education Studies
- Asymmetric Synthesis and Catalysis
- Immune Cell Function and Interaction
- Synthesis and Characterization of Heterocyclic Compounds
- Organophosphorus compounds synthesis
- Animal Behavior and Reproduction
- Chemical Synthesis and Analysis
- Cytomegalovirus and herpesvirus research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Organic Chemistry Cycloaddition Reactions
- Cyclopropane Reaction Mechanisms
- ATP Synthase and ATPases Research
SUNY Upstate Medical University
2013-2023
University of Bonn
2015-2019
Zurich University of the Arts
2016
Uppsala University
2011
Uppsala University Hospital
2005
Syracuse University
2003-2004
Board of the Swiss Federal Institutes of Technology
2000-2002
ETH Zurich
2000-2002
Max Planck Institute for Medical Research
1984-1998
Max Planck Society
1998
We have isolated rat brain cDNA clones encoding AE2, a homologue of the erythrocyte anion exchanger, band 3 (AE1). Immunocytochemistry and in situ hybridization reveal that, brain, AE2 expression is restricted to basolateral membrane choroid plexus epithelium. Expression full-length mouse COS-7 cells resulted chloride- bicarbonate-dependent alterations intracellular pH, demonstrating that Cl/HCO3 exchanger. Cation replacement studies indicate AE2-mediated exchange independent extracellular...
Mitochondrial biogenesis requires the import of >1,000 mitochondrial preproteins from cytosol. Most studies on protein are focused core machinery. Whether and how biophysical properties substrate affect overall efficiency is underexplored. Here, we show that traffic into mitochondria can be disrupted by amino acid substitutions in a single preprotein. Pathogenic missense mutations ADP/ATP translocase 1 (ANT1), its yeast homolog carrier 2 (Aac2), cause to accumulate along pathway, thereby...
Interspecific studies indicate that sperm morphology and other ejaculatory traits diverge more rapidly than types of character in Drosophila taxa. This pattern has largely been attributed to postcopulatory sexual selection involving interaction between the sexes. Such divergence suggested lead reproductive isolation among populations thus be an ‘engine speciation.’ Here, we test two critical predictions this hypothesis: (i) there is significant variation incipient species; (ii) interacting...
Nonsense-mediated decay (NMD) plays a fundamental role in the degradation of premature termination codon (PTC)-containing transcripts, but also regulates expression functional transcripts lacking PTCs, although such 'non-canonical' functions remain ill-defined and require identification factors targeting specific mRNAs to NMD machinery. Our work identifies stem cell-specific mRNA repressor protein TRIM71 as one these factors. an essential embryonic development is linked carcinogenesis. For...
Abstract Regulatory networks for differentiation and pluripotency in embryonic stem (ES) cells have long been suggested to be mutually exclusive. However, with the identification of many new components these ranging from epigenetic, transcriptional translational even post-translational mechanisms, cellular states early might not strictly bi-modal, but differentiating appear go through phases simultaneous expression stemness genes. Translational regulators such as RNA binding proteins (RBPs)...
Understanding the evolution of polyandry (mating with multiple males) is a major issue in study animal breeding systems. We examined adaptive significance Drosophila melanogaster, species well-documented costs mating which males generally cannot force copulations. found no direct fitness advantages polyandry. Females that mated had greater mean and different variance than females repeatedly same male. Subcomponents reproductive success, including fecundity, egg hatch rate, larval viability,...
Abstract 3.6′;3′.6";3".6‐Triphenanthrylen ( 2 ) wurde durch Ullmann‐Reaktion von 3.6‐Dijod‐phenanthren 6 sowie Umsetzung der aus 3.6‐Dibrom‐phenanthren 7 erhaltenen Di‐Grignard‐Verbindung mit wasserfreiem Kupfer(II)‐chlorid dargestellt. Trotz Möglichkeit einer annulen‐artigen Formulierung makrocyclischer Konjugation folgt den Eigenschaften , daß die π‐Elektronen‐Wechselwirkung weitgehend auf drei benzoiden Phenanthren‐Untereinheiten beschränkt ist. Zum Vergleich wurden analog verknüpfte Di‐...
Citrate lyase (EC 4.1.3.6) catalyzes the cleavage of citrate to acetate and oxaloacetate is composed three subunits (α, β, γ). The γ-subunit serves as an acyl carrier protein (ACP) contains prosthetic group 2'-(5' '-phosphoribosyl)-3'-dephospho-CoA, which attached via a phosphodiester linkage serine-14 in enzyme from Klebsiella pneumoniae. In this work, we demonstrate by genetic biochemical studies with Escherichia coli K. pneumoniae that conversion apo-ACP into holo-ACP dependent on two...
Virus-induced myositis is an emerging global affliction that remains poorly characterized with few treatment options. Moreover, muscle-tropic viruses often spread to the CNS, causing dramatically increased morbidity. Therefore, there urgent need explore genetic factors involved in this class of human disease. This report investigates critical innate immune pathways affecting murine virus-induced myositis. Of particular importance, key regulator src homology region 2 domain-containing...
We analyzed whether the phosphotransferase encoded by UL97 open reading frame of human cytomegalovirus (HCMV) alone is sufficient to confer ganciclovir (GCV) susceptibility a foreign virus. Two vaccinia virus recombinants (T1 and A5) containing frames from GCV-sensitive HCMV GCV-resistant strain were constructed. T1 exhibited phenotype in plaque reduction assays, whereas A5 did not. Moreover, T1-infected cell cultures showed strongly increased incorporation [14C]GCV triphosphate into...
Macrophages that lack connexin43 (Cx43), a gap junction protein, have been reported to exhibit dramatic deficiencies in phagocytosis. In this study, we revisit these findings using well-characterized macrophage populations. Cx43 knockout (Cx43(-/-)) mice die soon after birth, making the harvest of macrophages from adult Cx43(-/-) problematic. To overcome obstacle, used several strategies: heterozygous for deletion were crossed produce Cx43(+/+) (wild type [WT]) and fetuses. Cells isolated...
The γ‐subunit of citrate lyase (EC 4.1.3.6) contains the prosthetic group 2′‐(5″‐phosphoribosyl)‐3′‐dephospho‐CoA and serves as an acyl carrier protein (ACP). We recently showed that in Escherichia coli proteins CitG CitX are essential for holo‐ACP synthesis provided evidence catalyzes formation a precursor from ATP dephospho‐CoA, which is subsequently attached via phosphodiester linkage to apo‐ACP by CitX. Here we prove indeed conversion dephospho‐CoA adenine...
ABSTRACT The biosynthesis of the 2′-(5"-phosphoribosyl)-3′-dephospho-coenzyme A (CoA) prosthetic group citrate lyase (EC 4.1.3.6), a key enzyme fermentation, proceeds via initial formation precursor 2′-(5"-triphosphoribosyl)-3′-dephospho-CoA and subsequent transfer to apo-citrate with removal pyrophosphate. In Escherichia coli , two steps are catalyzed by CitG CitX, respectively, corresponding genes part gene cluster, citCDEFXG . homologous citCDEFG operon Klebsiella pneumoniae citX is...
Understanding the evolution of polyandry (mating with multiple males) is a major issue in study animal breeding systems. We examined adaptive significance Drosophila melanogaster, species well-documented costs mating which males generally cannot force copulations. found no direct fitness advantages polyandry. Females that mated had greater mean and different variance than females repeatedly same male. Subcomponents reproductive success, including fecundity, egg hatch rate, larval viability,...