A5057815226- Fullerene Chemistry and Applications
- Carbon Nanotubes in Composites
- bioluminescence and chemiluminescence research
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Spaceflight effects on biology
- Advanced Nanomaterials in Catalysis
- Chemical Reactions and Isotopes
- 3D Printing in Biomedical Research
- Biofield Effects and Biophysics
- Food Industry and Aquatic Biology
- Scientific Research and Studies
- Advanced oxidation water treatment
- Geochemistry and Elemental Analysis
- Nanoplatforms for cancer theranostics
- Thallium and Germanium Studies
- Computational Drug Discovery Methods
- Health, Environment, Cognitive Aging
- Metal-Organic Frameworks: Synthesis and Applications
- Radioactive element chemistry and processing
- Electrochemical sensors and biosensors
- Photoreceptor and optogenetics research
- Free Radicals and Antioxidants
- Cholinesterase and Neurodegenerative Diseases
- Molecular Communication and Nanonetworks
Siberian Federal University
2016-2024
Siberian Branch of the Russian Academy of Sciences
2024
Institute of Biophysics
2016-2024
Institute of Physics
2019-2024
Krasnoyarsk Scientific Center
2019-2023
The current study evaluates the role of reactive oxygen species (ROS) in bioeffects magnetite nanoparticles (MNPs), such as bare (Fe3O4), humic acids (Fe3O4-HA), and 3-aminopropyltriethoxysilane (Fe3O4-APTES) modified MNPs. Mössbauer spectroscopy was used to identify local surrounding for Fe atom/ions depth modification It found that Fe3O4-HA MNPs contain smallest, whereas Fe3O4-APTES largest amount Fe2+ ions. Bioluminescent cellular enzymatic assays were applied monitor toxicity...
Current paper presents biological effects of magnetite nanoparticles (MNPs). Relations MNP’ characteristics (zeta-potential and hydrodynamic diameters) with on bacteria their enzymatic reactions were the main focus. Photobacterium phosphoreum bacterial chosen as bioassays. Three types MNPs under study: bare Fe3O4, Fe3O4 modified 3-aminopropyltriethoxysilane (Fe3O4/APTES), humic acids (Fe3O4/HA). Effects studied at a low concentration range (< 2 mg/L) attributed to availability oxidative...
Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, a specific allotropic form carbon, bioactive compounds, and perspective basis for drug development. Our paper analyzes the antioxidant activity toxicity series fullerenols with different number oxygen substituents. Two groups were under investigation: (1) C60Oy(OH)x, C60,70Oy(OH)x, where x + y = 24-28 (2) Fe0,5C60Oy(OH)x, Gd@C82Oy(OH)x, 40-42. Bioluminescent cellular enzymatic assays (luminous marine bacteria...
Gadolinium (Gd)-containing fullerenols are perspective agents for magnetic resonance imaging and cancer research. They combine the unique paramagnetic properties of Gd with solubility in water, low toxicity antiradical activity fullerenols. We compared bioeffects two Gd-containing a different number oxygen groups—20 42: Gd@C82O20H14 Gd@C82O42H32. The bioluminescent bacteria-based assay was applied to monitor fullerenols, bioluminescence as signal physiological parameter, bacterial...
Firstly Fe 3 O 4 -MIL-88B(Fe) was studied, and the pro-oxidant role of ascorbic acid in enhancing its catalytic properties is investigated.
Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity fullerenols was studied in model solutions organic inorganic toxicants oxidative type – 1,4-benzoquinone potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 mixture two types С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based enzyme-based bioluminescent assays used to...
Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of water-soluble fullerene derivative, fullerenol, 10-12 oxygen groups (F10-12); its was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system used to study toxic antioxidant effects F10-12 at enzymatic level. Antioxidant characteristics were revealed in model solutions organic inorganic oxidizers. Low-concentration activation bioluminescence validated...
The Gd-containing metallofullerene derivatives are perspective magnetic resonance imaging contrast agents. We studied the bioeffects of a water-soluble fullerene derivative, gadolinium-endohedral fullerenol, with 40−42 oxygen groups (Gd@Fln). Bioluminescent cellular and enzymatic assays were applied to monitor toxicity antioxidant activity Gd@Fln in model solutions; bioluminescence was as signaling physiological parameter. inhibited at high concentrations (>2·10−1 gL−1), revealing lower...
Abstract A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH : FMN ‐oxidoreductase was developed to monitor toxicity antioxidant activity bioactive compounds. The enables studying toxic effects at the level biomolecules physicochemical processes, as well determining general oxidative types. Toxic detoxifying compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water‐soluble polyhydroxylated...
Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity different types (alpha, beta and gamma), bioactive compounds (humic substances fullerenols). Luminescence intensity is taken as a physiological parameter luminous organisms. High rates response can provide (1) proper number experiments under comparable conditions and, therefore, statistical processing, with this being highly important &lsquo;noisy&rsquo; low-dose...
Our current study aimed to adapt a bioluminescent bacteria-based bioassay monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) studied. Bioluminescence intensity was used as an indicator physiological activity in bacteria. Two additional methods used: reactive oxygen species (ROS) content estimated chemiluminescent luminol method, bacterial size...
Bioluminescence of isolated enzymes is a perspective phenomenon for biosensors development due to simplicity registration physiological parameter - light intensity. Enzyme-based bioluminescent assay widely used evaluate decrease in biochemical toxicities. Also the enzyme-based direct monitoring oxidative toxicity. This work considers antioxidant properties fullerenols, water-soluble polyhydroxylated derivatives fullerenes and pharmaceutical agents, solutions model inorganic organic toxicants...
This paper elucidates biochemical and physicochemical aspects of toxicity a redox-active compound in organisms. Sodium selenite (Na2SeO3) was chosen as model compound; coupled enzymatic system from luminous marine bacteria applied to imitate process. We demonstrated that Na2SeO3 suppressed bioluminescence the enzyme system; effective inhibition concentration 10–2 M. Besides that, decreased content reactive oxygen species (ROS) aqueous solutions (enzyme-free media) at >10–3 Addition...
Impact of sodium selenite (Na2 SeO3 ) (as a selenium compound) on bacterial bioluminescence and Photobacterium phosphoreum oxyanions biotransformation to elementary was investigated. Luminous bacteria were demonstrated be prospective tool for compounds’ toxicity monitoring, detoxification water solutions synthesis.
The inhibitory ability of three iron-containing coordination polymers (MOF, Fe3 O4 -MОF and MОF-Fe3 ) was analyzed using a bioluminescent enzymatic assay. results showed the neutrality all low ROS content non-functional polymers. Safe operation these for local drug delivery in therapy oncological diseases is assumed.