A5101454057- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Photosynthetic Processes and Mechanisms
- Insect-Plant Interactions and Control
- Ecology and Vegetation Dynamics Studies
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant Water Relations and Carbon Dynamics
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Plant and animal studies
- Peanut Plant Research Studies
- Plant biochemistry and biosynthesis
Lanzhou University
2024-2025
State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems
2025
Ministry of Agriculture and Rural Affairs
2025
Hainan University
2020-2024
Max Planck Institute for Chemical Ecology
2005-2023
Indian Institute of Science Education and Research Kolkata
2012-2019
Massachusetts Institute of Technology
2010-2011
Banaras Hindu University
2006-2010
Max Planck Society
2005-2010
Max Planck Institute for Plant Breeding Research
2009
Nicotine, the signature alkaloid of Nicotiana species responsible for addictive properties human tobacco smoking, functions as a defensive neurotoxin against attacking herbivores. However, evolution genetic features that contributed to assembly nicotine biosynthetic pathway remains unknown. We sequenced and assembled genomes two wild tobaccos, attenuata (2.5 Gb) obtusifolia (1.5 Gb), ecological models investigating adaptive traits in nature. show after Solanaceae whole-genome triplication...
The two closely related Arabidopsis transcription factors, WRKY18 and WRKY40, play a major partly redundant role in PAMP-triggered basal defense. We monitored the transcriptional reprogramming induced by powdery mildew fungus, Golovinomyces orontii, during early stages of infection with respect to WRKY18/40. Expression >1300 genes was differentially altered already 8 hours post (hpi), indicating rapid pre-penetration signaling between pathogen host. found that WRKY18/40 negatively affects...
The spot blotch disease of wheat is caused by Bipolaris sorokiniana , which an anamorph (teleomorph Cochliobolus sativus ). mainly occurs in warm, humid wheat‐growing regions, and the Eastern Gangetic Plains ( EGP ) South Asia a hotspot. Significant progress has been made recent years characterizing host–pathogen interaction. study pathogen's life cycle diversity have active area research. A number resistance sources also identified, characterized used for breeding. Although immunity not...
Deep-sequencing has enabled the identification of large numbers miRNAs and siRNAs, making high-throughput target a main limiting factor in defining their function. In plants, several tools have been developed to predict targets, majority them being trained on Arabidopsis datasets. An extensive systematic evaluation not made for suitability predicting targets species other than Arabidopsis. Nor, these evaluated prediction at genome level. We performance 11 computational identifying...
Summary Small RNAs are important regulators of plant development and resistance to viruses. To determine whether small mediate defense responses herbivore attack, we silenced the expression three RNA‐directed RNA polymerases (RdRs) in native tobacco Nicotiana attenuata by virus‐induced gene silencing. Larvae leaf‐chewing solanaceous specialist Manduca sexta grew faster on RdR1 ‐silenced plants than empty vector (EV) controls; silencing RdR3 2 had little no effect larval performance. NaRdR1...
Phytohormones mediate the perception of insect-specific signals and elicitation defenses during insect attack. Large-scale changes in a plant's transcriptome ensue, but how these are regulated remains unknown. Silencing RNA-directed RNA polymerase 1 (RdR1) makes Nicotiana attenuata highly susceptible to herbivores, suggesting that defense is under direct control small-RNAs (smRNAs). Using 454-sequencing, we characterized N. attenuata's smRNA before after wild-type (WT) RdR1-silenced (irRdR1)...
The SMc01113/YbeY protein, belonging to the UPF0054 family, is highly conserved in nearly every bacterium. However, function of these proteins still remains elusive. Our results show that share structural similarities with MID domain Argonaute (AGO) proteins, and might similarly bind a small-RNA (sRNA) seed, making special interaction phosphate on 5′-side suggesting they may form component bacterial sRNA pathway. Indeed, eliminating expression Sinorhizobium meliloti produces symbiotic...
Argonaute (AGO) proteins form the core of RNA-induced silencing complex, a central component smRNA machinery. Although reported from several plant species, little is known about their evolution. Moreover, these genes have not yet been cloned ecological model plant, Nicotiana attenuata, in which machinery to mediate important traits.Here, we only identify 11 AGOs N. further annotate 133 17 previously annotated Phytozome database, increase number 263 37 species. We report phylogenetic...
Abstract A halotolerant rhizobacteria, Klebsiella species (referred to MBE02), was identified that had a growth stimulation effect on peanut. To gain mechanistic insights into how molecular components were reprogrammed during the interaction of MBE02 and peanut roots, we performed deep RNA-sequencing. In total, 1260 genes differentially expressed: 979 up-regulated, whereas 281 down-regulated by treatment as compared uninoculated controls. large component regulated related phytohormone...
Abstract Soil conditioners are important for modifying soil degradation and optimizing the management of cultivated grasslands. may improve properties, which directly and/or indirectly affect plant growth biomass. However, different responses monocultures mixtures to still poorly understood. Here, joint effects species composition on biomass accumulation were evaluated in a manipulation experiment. Species compositions created by growing two typical forages, Onobrychis viciifolia Elymus...
Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize recruit sRNAs to interact with targets. In eukaryotes, Argonaute (AGO) proteins discharge such functions. The highly conserved YbeY RNase has structural similarities the MID domain of AGOs. A limited study had indicated that in Sinorhizobium meliloti ortholog regulates accumulation as well target mRNAs, raising possibility play a previously...
Summary Spot blotch disease, caused by Bipolaris sorokiniana , is an important threat to wheat, causing annual loss of ~17%. Under epidemic conditions, these losses may be 100%, yet the molecular responses wheat spot remain almost uncharacterized. Moreover, defense‐related phytohormone signaling genes have been poorly characterized in wheat. Here, we identified 18 central components salicylic acid ( SA ), jasmonic JA ethylene ET and enhanced disease susceptibility 1 EDS 1) pathways as well...
Argonautes (AGOs) associate with noncoding RNAs to regulate gene expression during development and stress adaptation. Their role in plant immunity against hemibiotrophic fungal infection remains poorly understood. Here, we explore the function of AGOs interaction wild tobacco (Nicotiana attenuata) a naturally occurring pathogen, Fusarium brachygibbosum. Among all AGOs, only transcripts AGO4 were elicited after infection. The disease progressed more rapidly AGO4-silenced (irAGO4) plants than...
The ubiquitous bacterial RNA-binding protein Hfq is involved in stress resistance and pathogenicity. In Sinorhizobium meliloti, essential for the establishment of symbiosis with Medicago sativa nitrogen fixation. A proteomic analysis identifies 55 proteins significantly affected expression hfq mutant; most them are cell metabolism or resistance. Important determinants oxidative resistance, such as CysK, Gsh, Bfr, SodC, KatB, KatC, a putative peroxiredoxine (SMc00072), downregulated mutant....
DICER-like (DCL) proteins produce small RNAs that silence genes involved in development and defenses against viruses pathogens. Which DCLs participate plant-herbivore interactions remains unstudied. We identified stably silenced four distinct DCL by RNAi Nicotiana attenuata (Torrey ex. Watson), a model for the study of interactions. Silencing DCL1 expression was lethal. Manduca sexta larvae performed significantly better on ir-dcl3 ir-dcl4 plants, but not ir-dcl2 plants compared to wild type...
Abstract The recently established Hainan Tropical Rainforest National Park has designated the gibbon ( Nomascus hainanus ) as its flagship species, providing new hope for recovery of last surviving population world's rarest primate. However, current monitoring methods are labour‐intensive and only conducted discrete periods, meaning that detailed information is still lacking on key parameters (such movement patterns, sleeping site selection home range size). Alternative techniques therefore...
Plants interact with arbuscular mycorrhizal fungi (AMF) and in doing so, change transcript levels of many miRNAs their targets. However, the identity an Argonaute (AGO) that modulates this interaction remains unknown, including Nicotiana attenuata. We examined how silencing NaAGO1/2/4/7/and 10 by RNAi influenced plant-competitive ability under low-P conditions when they AMF. Furthermore, roles seven miRNAs, predicted to regulate signaling phosphate homeostasis, were evaluated transient...
Summary RNA‐directed RNA‐polymerases (RdRs) are essential in small interfering RNA (siRNA) biogenesis and appear to be functionally specialized. We examined the consequences of silencing RdR2 Nicotiana attenuata with a field release, transcriptional, two‐dimensional proteomic metabolite analyses. Na RdR2‐ silenced plants ( irRdR2 ) had large reductions (46% wild type) 22–24‐nt RNAs (smRNAs), smaller (35, 23 26% 19–21, 25–27 28–30‐nt smRNAs, respectively. When planted into their native...