A5003776760- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Molecular Sensors and Ion Detection
- Glycosylation and Glycoproteins Research
- Chemical Synthesis and Analysis
- Genetics and Neurodevelopmental Disorders
- Advanced Electron Microscopy Techniques and Applications
- Studies on Chitinases and Chitosanases
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Crystallization and Solubility Studies
- Lipid Membrane Structure and Behavior
- X-ray Diffraction in Crystallography
- RNA and protein synthesis mechanisms
- Inorganic Fluorides and Related Compounds
- Advanced NMR Techniques and Applications
- Infant Nutrition and Health
- Electron and X-Ray Spectroscopy Techniques
- Endoplasmic Reticulum Stress and Disease
- Magnetism in coordination complexes
- Retinal Development and Disorders
- Nicotinic Acetylcholine Receptors Study
- RNA modifications and cancer
- Protein purification and stability
Centre for Cellular and Molecular Biology
2023-2025
National Institute of Technology Hamirpur
2025
National Centre for Cell Science
2014-2024
Academy of Scientific and Innovative Research
2023
Council of Scientific and Industrial Research
2023
Savitribai Phule Pune University
2019
Wellcome Trust/DBT India Alliance
2014-2015
National Institutes of Health
2009-2014
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2010-2014
Bioengineering Center
2012
Poor partitioning of macromolecules into the holes holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated grids, self-assembled monolayer whose surface properties can be controlled chemical modification. We demonstrate utility this approach drive ionotropic glutamate receptors holes, thereby enabling 3D analysis using cryo-EM methods.
The SMc01113/YbeY protein, belonging to the UPF0054 family, is highly conserved in nearly every bacterium. However, function of these proteins still remains elusive. Our results show that share structural similarities with MID domain Argonaute (AGO) proteins, and might similarly bind a small-RNA (sRNA) seed, making special interaction phosphate on 5′-side suggesting they may form component bacterial sRNA pathway. Indeed, eliminating expression Sinorhizobium meliloti produces symbiotic...
Small hydropower (SHP) systems are widely considered environmentally sustainable, but they still contribute to emissions during the construction and maintenance phase. This study evaluates life cycle acidification potential of different SHP projects in India using assessment (LCA) techniques. SO2eq from these vary between 0.065 0.325 g/kWh, depending on project type—run-of-river, canal-based, or dam-toe—as well as their respective size (capacity) location. The predictive models developed...
Abstract Particle identification and selection, which is a prerequisite for high-resolution structure determination of biological macromolecules via single-particle cryo-electron microscopy poses major bottleneck automating the steps determination. Here, we present generalized deep learning tool, CASSPER, automated detection isolation protein particles in transmission microscope images. This tool uses Semantic Segmentation collection visually prepared training samples to capture differences...
Glutamate receptor ion channels (iGluRs) are excitatory neurotransmitter receptors with a unique molecular architecture in which the extracellular domains assemble as dimer of dimers. The structure individual assemblies has been established previously for both isolated ligand-binding domain (LBD) and more recently larger amino terminal (ATD). How these dimers pack to form tetrameric intact iGluRs remained controversial. Using solved crystal structures GluK2 kainate ATD guide, we performed...
GluK3-kainate receptors are atypical members of the iGluR family that reside at both pre- and postsynapse play a vital role in regulation synaptic transmission. For better understanding structural changes underlie receptor functions, GluK3 were trapped desensitized resting/closed states structures analyzed using single particle cryo-electron microscopy. While has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound resting state with only two LBD domains...
We are reporting Co(ii) and Ni(ii) complexes of a pyridine containing aromatic macrocyclic triamide ligand, 3,6,9,15-tetraazabicyclo(9.3.1)pentadeca-1(15),11,13-triene-3,6,9-triacetamide (TPTA), as paramagnetic chemical exchange saturation transfer (paraCEST) MRI contrast agents. The synthesis characterization TPTA its reported. solution chemistry solid-state structure studied. Crystallographic data show that the [Co(TPTA)]·Cl2·2H2O complex (seven-coordinate, all four N atoms ring three...
Fe(II) and Ni(II) paraCEST contrast agents containing the di-pyridine macrocyclic ligand 2,2′,2″-(3,7,10-triaza-1,5(2,6)-dipyridinacycloundecaphane-3,7,10-triyl)triacetamide (DETA) are reported here. Both [Fe(DETA)]2+ [Ni(DETA)]2+ complexes were structurally characterized. Crystallographic data revealed seven-coordinated distorted pentagonal bipyramidal geometry of [Fe(DETA)]·(BF4)2·MeCN complex with five coordinated nitrogen atoms from ring two oxygen amide pendant arms. The...