A5023419445- RNA Research and Splicing
- Nuclear Structure and Function
- Protein Structure and Dynamics
- Advanced Electron Microscopy Techniques and Applications
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Enzyme Structure and Function
- Electron and X-Ray Spectroscopy Techniques
- Ubiquitin and proteasome pathways
- E-Government and Public Services
- Cardiomyopathy and Myosin Studies
- Internet Traffic Analysis and Secure E-voting
- Adipose Tissue and Metabolism
- Antibiotic Resistance in Bacteria
- Genomics and Chromatin Dynamics
- Fibroblast Growth Factor Research
- Microstructure and mechanical properties
- Adipokines, Inflammation, and Metabolic Diseases
- Bioinformatics and Genomic Networks
- Kruppel-like factors research
- Regulation of Appetite and Obesity
- HIV/AIDS drug development and treatment
- Animal Virus Infections Studies
- 14-3-3 protein interactions
National Centre for Cell Science
2017-2023
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2023
Savitribai Phule Pune University
2020-2021
Howard Hughes Medical Institute
2009-2011
Rockefeller University
2009-2011
Government of India
2008
Centre for DNA Fingerprinting and Diagnostics
2004
Centre for Cellular and Molecular Biology
2003
National Institute of Nutrition
2003
Indian Council of Medical Research
2003
Infections caused by multi-drug resistant Klebsiella pneumoniae are a leading cause of mortality and morbidity among hospitalized patients. In neonatal intensive care units (NICU), blood stream infections K. one the most common nosocomial to poor clinical outcomes prolonged hospital stays. Here, we describe an outbreak neonates admitted at NICU large tertiary in India. The involved 5 out 7 NICU. antibiotic sensitivity profiles revealed that all isolates were including carbapenems colistin....
Abstract Particle identification and selection, which is a prerequisite for high-resolution structure determination of biological macromolecules via single-particle cryo-electron microscopy poses major bottleneck automating the steps determination. Here, we present generalized deep learning tool, CASSPER, automated detection isolation protein particles in transmission microscope images. This tool uses Semantic Segmentation collection visually prepared training samples to capture differences...
Resistin, a small cysteine rich protein secreted by adipocytes, has been proposed to be link between obesity and type II diabetes modulating the insulin signaling pathway thus inducing resistance. Resistin protein, with 11 residues, was not significantly homologous at amino acid level any other known proteins. cDNA derived from human subcutaneous adipose tissue expressed in Escherichia coli as an N-terminal six-His-tag fusion protein. The overexpressed recombinant resistin purified...
Abstract India has become the third worst-hit nation by COVID-19 pandemic caused SARS-CoV-2 virus. Here, we investigated molecular, phylogenomic, and evolutionary dynamics of in western India, most affected region country. A total 90 genomes were sequenced. Four nucleotide variants, namely C241T, C3037T, C14408T (Pro4715Leu), A23403G (Asp614Gly), located at 5’UTR, Orf1a, Orf1b, Spike protein regions genome, respectively, predominant ubiquitous (90%). Phylogenetic analysis revealed four...
The central transport channel of the vertebrate nuclear pore complex (NPC) consists nucleoporins: Nup62, Nup54, and Nup58. coiled-coil domains in α-helical regions these nucleoporins are thought to be crucial for several protein-protein interactions NPC subcomplexes. In this study, we determined crystal structure domain rat Nup62 fragment (residues 362-425) 2.4 Å resolution. shows conserved as a parallel three-helix bundle Nup62(362-425) fragment. On basis our size exclusion chromatography...
The paper describes an Enterprise Architecture1 for E-Government2, Governments across the world who intend to provide better service delivery their business, citizens and other stakeholders.
Abstract Nuclear pore complex (NPC) is the largest multimeric protein assembly of eukaryotic cell, which mediates nucleocytoplasmic transport. The constituent proteins this (nucleoporins) are present in varying copy numbers to give a size from ~ 60 MDa (yeast) 112 (human) and share common ancestry with other membrane‐associated complexes such as COPI/COPII thus same structural folds. However, nucleoporins across species exhibit very low percentage sequence similarity reflects their distinct...
The nuclear pore complex (NPC) is a highly modular assembly of 34 distinct nucleoporins (Nups) to form versatile transport channel between the nucleus and cytoplasm. Among them, Nup62 known as an essential component for transport, Nup93 proper envelope assembly. These Nups constitute various NPC subcomplexes such central (CTC), cytoplasmic ring (CR), inner (IR). However, how they play their roles in activity not clear. Here we delineated interacting regions conducted biochemical...
A policy framework is the backbone of public governance and a major contributor to its quality. Such particularly required in areas where seeks technology support, as case for Electronic Governance (e-Governance). This paper explains need put place comprehensive set policies, presents model interventions supporting e-Governance development. The comprises classification policies based on their nature applicability, describes core which are required. also three scenarios using model: (1) tool...
Decrypting the interface residues of protein complexes provides insight into functions proteins and, hence, overall cellular machinery. Computational methods have been devised in past to predict using amino acid sequence information, but all these majorly applied for prokaryotic complexes. Since composition and rate evolution primary is different between prokaryotes eukaryotes, it important develop a method specifically eukaryotic Here, we report new hybrid pipeline predicting...
ABSTRACT The central transport channel (CTC) of nuclear pore complexes (NPCs) is made up three nucleoporins Nup62, Nup58 and Nup54. In which manner capacity, these form the CTC, not yet clear. We explored CTC Nups from various species observed that distinct biochemical characteristics are evolutionarily conserved. Moreover, comparative analysis showed stoichiometric combinations Nup54 coexisting together. conserved amino‐terminal domain mammalian Nup93 crucial for anchorage its localization...
Sequential alteration in the expression levels of cell cycle regulatory proteins is crucial for faithful progression to maintain cellular homeostasis. F‐box protein β‐TrCP1 known control several important proteins. However, how function regulated spatiotemporal manner during remains elusive. Here, we show that oscillate with a minimum level at G1 and S phases cycle. Using biochemical, flow cytometry, immunofluorescence techniques, found oscillation controlled by another FBXW8. FBXW8 directs...
Mycobacterium tuberculosis (H37Rv), the causative agent of dreaded disease tuberculosis, contains three thioredoxins and a single thioredoxin reductase. Thioredoxin reductase is member pyridine-nucleotide disulfide oxidoreductase family flavoenzymes. The gene with His tag at C-terminus was expressed in Escherichia coli purified. dimeric (70 kDa) protein incubated 10 mM DTT for 30 min then crystallized using hanging-drop vapour-diffusion method presence 15% PEG 3350 phosphate–citrate buffer...
Abstract Single-particle cryo-electron microscopy has emerged as the method of choice for structure determination proteins and protein complexes. However, particle identification selection which is a prerequisite achieving high-resolution still poses major bottleneck automating steps determination. Here, we present generalised deep learning tool, CASSPER, automated detection isolation particles in transmission microscope images. This tool uses Semantic Segmentation collection visually...
Abstract Living cells require a dynamic and precisely regulated actin cytoskeleton to carry out normal cellular functions. In addition well-established regulators, such as nucleators, capping proteins, bundlers, likely have uncharacterized modulators that regulate dynamics, the detailed functions of which are not yet fully understood. this study, we conducted biochemical exploration identify actin-regulatory activity Kaptin (KPTN), protein known co-localize with actin-rich structures at...
Abstract Computational methods have been devised in the past to predict interface residues using amino acid sequence information but majorly applied for prokaryotic protein complexes. Since composition and rate of evolution primary are different between prokaryotes eukaryotes, it is important develop a method specifically eukaryotic Here we report new hybrid pipeline prediction protein-protein interaction interfaces from alone based on framework Co -evolution, machine learning ( R andom...
ABSTRACT The nuclear pore complex (NPC) is a highly modular assembly of 34 distinct nucleoporins (Nups), to form versatile transport channel between the nucleus and cytoplasm. Among them, Nup62 known as an essential component for while, Nup93 proper envelope assembly. These Nups constitute various NPC subcomplexes: such central (CTC), cytoplasmic ring (CR) inner (IR). However, how they play their role in activity not clear. Here we delineated interacting regions, conducted biochemical...
Summary Human nuclear pore complexes are composed of ∼32 distinct nucleoporins to facilitate bidirectional nucleo-cytoplasmic transport. Many them have been associated with various human diseases such as an inherited mutation (R391H) in Nup155 is shown the clinical cause atrial fibrillation and sudden cardiac arrest. Due lack structural knowledge mechanistic insights, roles Nups NPC assembly relevance very restricted. Here, we show cryo-EM structure at 5.2-5.7. Å resolution deciphered from 3...