A5060631437- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Metal complexes synthesis and properties
- Computational Drug Discovery Methods
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Synthetic Organic Chemistry Methods
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- Cell death mechanisms and regulation
- Protein Interaction Studies and Fluorescence Analysis
- Catalytic Cross-Coupling Reactions
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- DNA Repair Mechanisms
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Protein Degradation and Inhibitors
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Molecular Sensors and Ion Detection
- Crystallography and molecular interactions
National Centre for Cell Science
2016-2025
Savitribai Phule Pune University
2013-2024
University of Massachusetts Chan Medical School
2009
Howard Hughes Medical Institute
2009
Indian Institute of Technology Bombay
2003-2007
University of California, Santa Barbara
2005
Thomas Jefferson University
1995-1998
Decorin, a small leucine-rich proteoglycan, is capable of suppressing the growth various tumor cell lines when expressed ectopically. In this report, we investigated biochemical mechanism by which decorin inhibits cycle progression. A431 squamous carcinoma cells, proteoglycan or protein core induced marked suppression, either exogenously added endogenously produced transgene. Decorin caused rapid phosphorylation EGF receptor and concurrent activation mitogen-activated (MAP) kinase signal...
Bacterial diseases are among the leading causes of human death. The development antibiotic resistance greatly contributes to high mortality rate, and thus, discovery antibacterial drugs with novel mechanisms action is needed. In this study, we found that sanguinarine, a benzophenanthridine alkaloid, strongly induced filamentation in both Gram-positive Gram-negative bacteria prevented bacterial cell division by inhibiting cytokinesis. Sanguinarine did not perturb membrane structure...
The rapid progress in the cloning of proteoglycan genes has enabled investigators to examine depth functional roles these polyhedric molecules play control cell proliferation. Decorin, a leucine-rich expressed by most connective tissues, is prototype molecule that regulates cellular growth via two mechanisms: modulation factor activity and matrix assembly. We now provide direct evidence human colon cancer cells stably transfected with decorin cDNA exhibit marked suppression transformed...
Decorin belongs to a family of secreted, small, leucine-rich proteoglycans that affect matrix assembly and cellular growth. Ectopic expression decorin proteoglycan or protein core as mutated form lacking any glycosaminoglycan side chains induced growth suppression in neoplastic cells various histogenetic origins, including tumor derived from gastrointestinal, genital, skeletal, cutaneous, bone marrow tissues. Exogenously added recombinant also suppressed overall the parental cell lines. In...
Cu(II) complexes of three tridentate ligands, L1, L2 and L3,[ N-((1H-imidazole-2-yl)methyl)-2-(pyridine-2-yl)ethanamine; L2, N-((1-methyl-1H-imidazole-2-yl)methyl)-2-(pyridine-2-yl)ethanamine; L3, 2-(pyridine-2-yl)-N-((pyridine-2-yl)methyl)ethanamine] respectively, were synthesized characterized. The single crystal X-ray structure complex 1 reveals the pseudo octahedral coordination geometry around copper center. Absorption fluorescence experimental evidence show good DNA binding propensity...
The antifungal drug griseofulvin inhibits mitosis strongly in fungal cells and weakly mammalian by affecting mitotic spindle microtubule (MT) function. Griseofulvin also blocks cell-cycle progression at G 2 /M induces apoptosis human tumor cell lines. Despite extensive study, the mechanism which remains unclear. Here, we analyzed ability of to inhibit proliferation affect MT polymerization organization HeLa together with its dynamic instability vitro . inhibited prometaphase/anaphase...
Abstract The Bcl-2 family of proteins consists both antiapoptotic and proapoptotic factors, which share sequence homology within conserved regions known as domains. Interactions between members, well with other proteins, regulate apoptosis through control mitochondrial membrane permeability release cytochrome c. Here we identify a novel regulator that lacks domains but acts by binding modulating its activity. To regulators apoptosis, performed expression profiling in human primary...
Significance The tumor suppressor protein p53 plays a critical role in protecting humans from cancer. In response to cellular stresses, such as DNA damage, levels increase and induce variety of protective biological responses. unstressed cells, is maintained at low level by MDM2, that interacts with promotes its degradation. Following MDM2 degraded rapidly, resulting increased levels. Because the essential modulating function, it understand how are regulated. Here we show that, following...
Abstract The Tumor suppressor SMAR1 (scaffold matrix attachment region binding protein 1) has a crucial role in maintaining genomic stability, cell cycle progression and apoptosis.Our previous finding showed that it is highly suppressed higher grade of cancer. However, the underlying mechanism this suppression was not well understood. In study, we show expression levels are controlled at proteasomal level by five RING finger E3 ubiquitin ligases including, Cdc20, substrate receptor ligase...
// Tushar H. More 1, 2 , Sourav RoyChoudhury 3 Joel Christie 1 Khushman Taunk Anupama Mane 4 Manas K. Santra 5 Koel Chaudhury and Srikanth Rapole Proteomics Lab, National Center for Cell Science, Ganeshkhind, Pune 411007, MH, India Savitribai Phule University, School of Medical Science Technology, Indian Institute Kharagpur 721302, WB, Grant Foundation, Ruby Hall Clinic, 411001, Cancer Biology Epigenetics Correspondence to: Rapole, email: rsrikanth@nccs.res.in Keywords: Invasive ductal...
Globally, breast cancer is the second most common among women. Although biomarker discoveries through various proteomic approaches of tissue and serum samples have been studied in cancer, urinary proteome alterations are least studied. Urine being a noninvasive biofluid significant source proteins, it has potential early diagnosis cancer. This study used complementary quantitative gel-based gel-free to find panel protein markers that could discriminate HER2 enriched (HE) subtype from healthy...
Two new Zn (II)‐dicyanamide (dca) 1‐D chain coordination polymers (CPs), [Zn (L OMe )(μ 1 ‐dca)(μ 1,5 ‐dca)] n (1) and OEt (2) have been successfully synthesized from bicompartmental Schiff base ligands N,N ′ ‐Bis(3‐methoxysalicylidenimino)‐1,3‐diaminopropane (H 2 L ), ‐Bis(3‐ethoxysalicylidenimino)‐1,3‐diaminoproane ) respectively structurally characterized using various spectroscopic protocols like H NMR, IR, Raman, UV–Vis, fluorescence as well elemental analysis, TGA, PXRD SCXRD studies....
The increasing resistance of bacteria to commercially available antibiotics threatens patient safety in healthcare settings. Perturbation ion homeostasis has emerged as a potential therapeutic strategy fight against antibacterial and other channelopathies. This study reports the development 8-aminoquinoline (QN) derivatives their transmembrane Zn2+ transport activities. Our findings showed that potent QN-based transporter exhibits promising properties Gram-positive with reduced hemolytic...
Abstract Cycloartenone and cycloartenol, two plant‐derived triterpenoids possessing cyclopropane ring were isolated from the latex of Artocarpus heterophyllus (jackfruit). Cycloartenol was chemically modified to synthesize dihydrocycloartenol via acetylation deacetylation methodology followed by hydrogenation. Hydroxyl group present in cycloartenol also employed for nitro benzoate formation. All synthesized compounds assayed against MCF7 breast cancer cell line their anticancer properties.
Significance Multiple studies highlight the role of various proteins in regulation alternative splicing; however, regulatory distinct posttranslational modifications during splicing that contribute to tumorigenesis is enigmatic. Here we report a previously unidentified noncanonical mechanism modulated by deacetylation RNA-binding protein Sam68 (Src-associated substrate mitosis 68 kDa) via Scaffold/matrix-associated region-binding 1 (SMAR1)–histone deacetylase 6 (HDAC6) complex. SMAR1 complex...