Magnetic nanoparticles of γ-MnxFe2–xO3 (0 ≤ x 1.3) have been synthesized successfully using a single step process wherein the respective inorganic salts were thermally decomposed in ethylene glycol at 200 °C. Single phase formation is evident as prepared dried samples without any further treatment. XRD line broadening along with TEM suggest that particle size below 30 nm. Both magnetic and data support substitution Mn3+ ions tetrahedral site γ-Fe2O3. Improved magnetization value (78 emu g–1)...

The antifungal drug griseofulvin inhibits mitosis strongly in fungal cells and weakly mammalian by affecting mitotic spindle microtubule (MT) function. Griseofulvin also blocks cell-cycle progression at G 2 /M induces apoptosis human tumor cell lines. Despite extensive study, the mechanism which remains unclear. Here, we analyzed ability of to inhibit proliferation affect MT polymerization organization HeLa together with its dynamic instability vitro . inhibited prometaphase/anaphase...

Curcumin, a component of turmeric, has potent antitumor activity against several tumor types. However, its molecular target and mechanism antiproliferative are not clear. Here, we identified curcumin as novel antimicrotubule agent. We have examined the effects on cellular microtubules reconstituted in vitro. Curcumin inhibited HeLa MCF-7 cell proliferation concentration-dependent manner with IC(50) 13.8 +/- 0.7 microm 12 0.6 microm, respectively. At higher inhibitory concentrations (> 10...

Abstract Background Griseofulvin, an antifungal drug, has recently been shown to inhibit proliferation of various types cancer cells and tumor growth in athymic mice. Due its low toxicity, griseofulvin drawn considerable attention for potential use chemotherapy. This work aims understand how suppresses microtubule dynamics living sought elucidate the antimitotic antiproliferative action drug. Methods The effects on individual microtubules live MCF-7 were measured by confocal microscopy....

Abstract La 1− x Sr MnO 3 , a ferromagnet with high magnetization and Curie temperature T C below 70°C, enables its use for magnetic hyperthermia treatment of cancer possibility in vivo control. We found that 0.73 0.27 particles size range 20–100 nm showed saturation around 38 emu/g at 20 kOe value 45°C. Aqueous suspension these nanoparticles was prepared using polymer, acrypol 934, the biocompatibility examined HeLa cells. A good heating ability obtained presence AC field, it to increase...

We found that benomyl, a benzimidazole fungicide, strongly suppressed the reassembly of cold‐depolymerized spindle microtubules in HeLa cells. Benomyl perturbed microtubule‐kinetochore attachment and chromosome alignment at metaphase plate. also significantly decreased distance between sister kinetochore pairs cells increased level checkpoint protein BubR1 region, indicating benomyl caused loss tension across kinetochores. In addition, intercentrosomal mitotic blocked mitosis. Further, we...

The unusual di-bromo product, <bold>5b</bold>, confirmed by spectral analysis, showed the most potency with lowest IC₅₀ and MIC values, excellent activity comparable to standard antibacterial drug, tetracycline.

Tin(IV) porphyrin scaffolds having long-chain (C8 and C16) alkoxyphenyl group at one meso position were synthesized with axial dichloro dihydroxo ligands. Modifications respect to the alkoxy chain length ligands investigated using UV–vis fluorescence spectroscopy as well cyclic voltammetry. Significantly higher molar absorption coefficients noted whereas quantum yields observed for C8 substituents. Electrochemical redox data revealed that ring became difficult reduce consequent presence of...

The newly synthesized water-soluble cyclodiphosphazane ligands and their gold(I) complexes inhibit HeLa cell proliferation by activating p53 protein inducing apoptosis.

Shabeeba M Ashrafa & Krishnan Rathinasamya* http://orcid.org/0000-0001-9685-135Xa School of Biotechnology, National Institute Technology Calicut, India

Photophysical studies, fluorescence imaging, single crystal X-ray structure analysis and DFT calculations revealed that compounds<bold>2</bold>and<bold>3</bold>show enhanced phototoxicity towards<italic>Candida albicans</italic>compared to compound<bold>1</bold>.

Berberine has been used traditionally for its diverse pharmacological actions. It exhibits remarkable anticancer activities and is currently under clinical trials. In this study, we report that the activity of berberine could be partly due to inhibitory actions on tubulin microtubule assembly. inhibited proliferation HeLa cells with an IC50 18 μM induced significant depolymerization interphase mitotic microtubules. At IC50, exerted a moderate G2/M arrest block as detected by...

Benomyl, a tubulin-targeted antimitotic antifungal agent, belongs to the benzimidazole group of compounds, which are known inhibit binding colchicine tubulin. Therefore, benomyl was thought bind at or near colchicine-binding site on However, recent mutational studies in yeast and fluorescence involving competitive goat brain tubulin suggested that may distinct from site. We set out examine whether sites using human cervical cancer (HeLa) cell line with thinking these agents should exert...

Colloidal silver nanoparticles (AgNPs) have attracted much attention in recent years as diagnostics and new drug delivery system cancer medicine. To study the effects of plumbagin (PLB), a relatively non-toxic napthaquinone isolated from roots Plumbago indica human cervical cell line developed formulation to enhance its cytotoxic activities. Silver were synthesised by chemical reduction method complexed with PLB. Both AgNPs complex PLB-AgNPs characterised dynamic light scattering,...

Abstract Objectives The aim of this study was to elucidate the antimitotic mechanism zerumbone and investigate its effect on HeLa cells in combination with other mitotic blockers. Materials methods fluorescence microscopy were used analyse cancer cell lines. Cellular internalization investigated using FITC‐labelled zerumbone. interaction tubulin characterized spectroscopy. Chou Talalay equation calculate index. Results Zerumbone selectively inhibited proliferation an IC 50 14.2 ± 0.5 μmol/L...

The large-bite bis(phosphite) ligand [{(-OC(10)H(6)(mu-S)C(10)H(6)O-)P{mu-(-OC(10)H(6)(mu-S)C(10)H(6)O-)}P(-OC(10)H(6)(mu-S)C(10)H(6)O-)}] (Pinsertion markP) () was obtained by the reaction of PCl(3) and thiobis(2,2'-naphthol) (). stoichiometric reactions with elemental sulfur selenium afforded corresponding chalcogenide derivatives [(E)Pinsertion markP(E)] (, E = S; , Se) in good yield. Treatment two equivalents [ClAu(SMe(2))] a dinuclear complex [ClAu(Pinsertion markP)AuCl] (), whereas 1 :...