A5029967823- Wheat and Barley Genetics and Pathology
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Food composition and properties
- Plant responses to water stress
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Fermentation and Sensory Analysis
- Plant responses to elevated CO2
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Advanced Proteomics Techniques and Applications
- Phytochemicals and Antioxidant Activities
- Metabolomics and Mass Spectrometry Studies
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- GABA and Rice Research
- Genetics and Plant Breeding
- Plant Genetic and Mutation Studies
- Horticultural and Viticultural Research
Tibet Academy of Agricultural and Animal Husbandry Sciences
2014-2023
Tibet University
2021
Tibet Autonomous Region People's Hospital
2015-2016
Tibetan barley (Hordeum vulgare L., qingke) is the principal cereal cultivated on Plateau for at least 3,500 years, but its origin and domestication remain unclear. Here, based deep-coverage whole-genome published exome-capture resequencing data a total of 437 accessions, we show that contemporary qingke derived from eastern domesticated it introduced to southern Tibet most likely via north Pakistan, India, Nepal between 4,500 years ago. The low genetic diversity suggests can be excluded as...
Significance The draft genome of Tibetan hulless barley provides a robust framework to better understand Poaceae evolution and substantial basis for functional genomics crop species with large genome. expansion stress-related gene families in implies that it could be considered as an invaluable resource aiding stress tolerance improvement Triticeae crops. Genome resequencing revealed extensive genetic diversity germplasm divergence sequenced genomes from other geographical regions....
Hulless barley, also called naked is an important cereal crop worldwide, serving as a healthy food both for human consumption and animal feed. Nevertheless, it often suffered from drought stress during its growth development, resulting in drastic reduction barley yields. Therefore, study on molecular mechanism of hulless drought-tolerance very increasing production. To investigate drought-resistance, this examined co-regulated mRNAs that show change expression pattern under early well water,...
Tibetan hulless barley is widely grown in the extreme environmental conditions of Qinghai-Tibet Plateau which characterized by cold, high salinity, and drought. Osmotic stress always occurs simultaneously with drought its tolerance a vital part tolerance. The diversity metabolites leading to osmotic was using widely-targeted metabolomics tolerant (XL) sensitive (D) accessions submitted polyethylene glycol. XL regulated more diverse set than D, may promote establishment robust system cope XL....
Tibetan hulless barley (qingke) is an important food crop in the plateau. However, it often suffers from drought stress resulting reduction of production because extreme plateau environment. To elucidate molecular mechanisms underlying resistance qingke, transcriptomic and metabolomic responses drought-sensitive (D) drought-resistant (XL) accessions were characterized experiments with a time course design. The phenylpropanoid pathway was reprogrammed by downregulating lignin increasing...
Powdery mildew is a fungal disease that represents ubiquitous threat to crop plants. Transcriptomic and metabolomic analyses were used identify molecular physiological changes in Tibetan hulless barley response powdery mildew. There 3418 genes 405 metabolites differentially expressed between the complete resistance cultivar G7 sensitive Z13. Weighted gene coexpression network analysis was carried out, enriched five four major modules Z13, respectively. Further showed phytohormones,...
Salinity stress represents one of the most harmful abiotic stresses for agricultural productivity. Tibetan hulless barley is an important economic crop widely grown in highly stressful conditions Qinghai-Tibet Plateau and often challenged by salinity stress. To investigate temporal metabolic responses to barley, we performed a targeted metabolomic analysis 72 leaf samples from two contrasting cultivars. We identified 642 compounds 57 % which were affected salt cultivars, principally amino...
Abstract Hulless barley ( Hordeum vulgare L. var. nudum ) is a variety that has loose husk cover of the caryopses. Because ease in processing and edibility, hulless been locally cultivated used as human food. For example, Tibetan Plateau, staple food for essential livestock feed. Although draft genome sequenced, assembly remains fragmented. Here, we reported an improved high-quality annotation using more than 67X PacBio long-reads. The N50 contig length new at least 19 times larger other...
Abstract Stress emanating from drought condition is one of the inevitable conditions that occurs in many environments and spread across borders results severe hindrance to morphology, physiology, biochemistry molecular attributes plants. Highland barley tolerance has been demonstrated be a polygenic related characteristic genetic composition can assist dissecting gene network(s) controlling needs adequately understood. DNA affinity purification sequencing (DAP-seq) shown contribute ways...
Abstract Background Tibetan hull-less barley (Hordeum vulgare L. var. nudum) is one of the primary crops cultivated in mountains Tibet and encounters low temperature, high salinity, drought. Specifically, drought major abiotic stresses that affect limit growth. Osmotic stress often simultaneously accompanied by conditions. Thus, to improve crop yield, it critical explore molecular mechanism governing responses osmotic/drought Findings In this study, we used quantitative proteomics...
Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In study, we aimed understand the functions and pathways genes involved disease resistance transcriptome sequencing a Tibetan landrace with high mildew. A total 831 significant differentially expressed were found infected seedlings, covering 19 functions. Either "cell," "cell part," "extracellular region" cellular...
Nitrogen (N) deprivation or excess can lead to dramatic phenotype change, disrupt important biological processes, and ultimately limit plant productivity. To explore genes in Tibetan hulless barley responsive varied N stress, we utilized a comparative transcriptomics method investigate gene expression patterns under three nitrate treatments. The transcriptome of the control (optimal-nitrate, ON) sample was compared with that free-nitrate (FN), low-nitrate (LN) high-nitrate (HN) treatment...
Clarifying genetic diversity in a large germplasm resource plays important roles experimental designs that provides flexible utility fundamental research and breeding crops. However, the work is limited due to small collections of barley are insufficient representatives.In present study, we collected 562 hulless (Hordeum vulgare L.) accessions with worldwide geographic origins evaluated their variability relatedness based on 93 simple sequence repeat (SSR) markers. In an integrated analysis...
Cereal grains accumulate anthocyanin during developmental process. The content increases at grain filling stages to develop coloration in cereals. However, biosynthesis responsible for coloring and its regulatory mechanisms controlled by structural functional genes remain unclear. Therefore, this study aimed explore the global map of metabolic changes linked Tibetan hulless barley (qingke) using an integrative metabolome transcriptome approach. Grains from three colored qingke cultivars...
The hull-less barley (Qingke) is widely planted as a staple food crop in the Tibetan area, China, and grains contains high content of β-glucan (BG). mechanisms BG synthesis accumulation qingke has not been studied at protein level. This study characterized proteins associated with during seed development. proteome profiles seeds taken 20, 30, 40 days after flowering were compared using TMT-based quantitative proteomics. A total 4283 identified, 759 being differentially expressed (DEPs)...
Qingke (Tibetan hulless barley, Hordeum vulgare L. var. nudum) is the primary food crop on Tibet Plateau, long-term drought and other harsh environments makes qingke an important resource for study of abiotic resistance. Here, we evaluated sensitivity 246 varieties. Genome-wide association studies (GWAS) found that root-specific expressed gene CYP84 may be involved in regulation Based widely targeted metabolic profiling, identified 2,769 metabolites leaves, which 302 were significantly...
Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes reduction in production. In this study, full-length cDNA gene encoding syntaxin-associated protein was cloned from leaves drought-resistant variety barley, "Himalaya 10"; its expression evaluated during drought stress and rehydration via real-time PCR. The HbSYR1 sequence 1300 bp length, included an 840-bp open reading frame that encoded 279 amino acids. Sequence analysis predicted molecular weight to be...
Hulless barley is an important crop cereal in Tibetan, China.Drought a major abiotic stress production.In this study, we cloned the drought-related HbSINA4 gene from variety 'Himalaya 10' and analyzed its expression patterns under different drought rehydration conditions.The cDNA of was 1052 bp long, including open reading frame 771 that encoded protein 256 amino acids.The molecular weight predicted to be 29.53 kDa theoretical pI 8.32.Bioinformatic analysis showed contained kinase domain...
Abstract Hullessbarley ( Hordeum vulgare L.) is a barley cultivar, serving as one of the major food crops worldwide. The main nutrients in hulless are starch, protein, and β‐glucan, contents which vary considerably among different cultivars. However, potential molecular mechanisms that underlie these variations nutrient still insufficiently clear. In this study, content two cultivars (‘Zangqing 2000’ ‘C2’) was analyzed, quantitative tandem mass tag‐LC‐MS/MS proteomics bioinformatics analysis...