A5072459952- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Viral Infections and Vectors
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Mosquito-borne diseases and control
- Photoreceptor and optogenetics research
- Galectins and Cancer Biology
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Advanced Electron Microscopy Techniques and Applications
- Fire effects on ecosystems
- Disaster Management and Resilience
- Viral Infections and Outbreaks Research
- Plant biochemistry and biosynthesis
- Polyamine Metabolism and Applications
- Wastewater Treatment and Nitrogen Removal
Fudan University
2024-2025
Zhongshan Hospital
2024-2025
State Key Laboratory of Medical Neurobiology
2024-2025
University of California, Berkeley
2020-2024
QB3
2021
Madison Group (United States)
2018
University of Wisconsin–Madison
2015-2017
Tsinghua University
2016
Institution of Structural Engineers
2013
Shanghai Institute for Science of Science
2011
The activated sludge process is an essential for treating domestic and industrial wastewaters in most wastewater treatment plants (WWTPs). This consists of a mixture general special microorganisms form complex enrichment population. Thus, the exploration microbial communities crucial to improve performance process. In this study, we investigated phylogenetic diversity metabolic potential full-scale WWTPs. Four 16S rRNA gene clone libraries were constructed from samples. all samples,...
Phosphorus in crucial for all living organisms. In vertebrate, cellular phosphate homeostasis is partly controlled by XPR1, a poorly characterized inositol pyrophosphate-dependent exporter. Here, we report the cryo-EM structure of human which forms loose dimer with 10 transmembrane helices (TM) each protomer. The consists scaffold domain (TM1, TM3-4) and core (TM2, TM5-10) structurally related to ion-translocating rhodopsins. Bound observed tunnel within at narrow point that separates into...
Bunyamwera virus (BUNV), which belongs to the genus Orthobunyavirus , is prototypical of Bunyaviridae family. Similar other negative-sense single-stranded RNA viruses, bunyaviruses possess a nucleocapsid protein (NP) facilitate genomic encapsidation and replication. The structures two NPs members different genera within family have been reported. However, their structures, RNA-binding features, functions beyond binding significantly differ from one another. Here, we report crystal structure...
The O-linked β-N-acetyl glucosamine (O-GlcNAc) modification dynamically regulates the functions of numerous proteins. A single human enzyme glucosaminase (O-GlcNAcase or OGA) hydrolyzes this modification. To date, it remains largely unknown how OGA recognizes various substrates. Here we report structures in complex with each four distinct glycopeptide substrates that contain a O-GlcNAc on serine threonine residue. Intriguingly, these glycopeptides bind bidirectional yet conserved...
Hantaviruses, which belong to the genus Hantavirus in family Bunyaviridae, infect mammals, including humans, causing either hemorrhagic fever with renal syndrome (HFRS) or hantavirus cardiopulmonary (HCPS) humans high mortality. encodes a nucleocapsid protein (NP) encapsidate genome and form ribonucleoprotein complex (RNP) together viral polymerase. Here, we report crystal structure of core domains NP (NPcore) encoded by Sin Nombre virus (SNV) Andes (ANDV), are two representative members...
Carbonic anhydrase IV (Car4) is a newly identified receptor that allows adeno-associated virus (AAV) 9P31 to cross the blood-brain barrier and achieve efficient infection in central nervous system (CNS) mouse models. However, molecular mechanism by which engineered AAV capsids with 7-mer insertion variable region (VR) VIII recognize these novel cellular receptors unknown. Here we report cryo-EM structures of AAV9P31 its complex Mus musculus Car4 at atomic resolution utilizing block-based...
Abstract Tweety homologs (TTYHs) comprise a conserved family of transmembrane proteins found in eukaryotes with three members (TTYH1-3) vertebrates. They are widely expressed mammals including at high levels the nervous system and have been implicated cancers other diseases epilepsy, chronic pain, viral infections. TTYHs reported to form Ca 2+ - cell volume-regulated anion channels structurally distinct from any characterized protein potential roles adhesion, migration, developmental...
Pseudomonas mendocina NK-01 can synthesize medium-chain-length polyhydroxyalkanoate (PHA(MCL)) and alginate oligosaccharides (AO) simultaneously from glucose under conditions of limited nitrogen. Here, we report the complete sequence 5.4-Mbp genome NK-01, which was isolated farmland soil in Tianjin, China.
Abstract Voltage-sensing phosphatases (VSPs) dephosphorylate phosphoinositide (PIP) signaling lipids in response to membrane depolarization. VSPs possess an S4-containing voltage sensor domain (VSD), resembling that of voltage-gated cation channels, and a lipid phosphatase (PD). The mechanism by which turns on enzyme activity is unclear. Structural analysis modeling suggest several sites VSD-PD interaction could couple sensing catalysis. Voltage clamp fluorometry reveals voltage-driven...
Phosphorus is an essential element for all living organisms. While inorganic phosphate (Pi) cellular import well documented, export poorly characterized despite the recent discovery of inositol pyrophosphate (PP-IP)-dependent Pi exporter XPR1. In this study, we determined cryo-EM structures XPR1 forms a loose dimer, with each protomer containing 10 transmembrane helices (TMs) that can be divided into peripheral domain (TM1, 3 and 4) core (TM2 TM5-10) structurally related to ion-translocating...
Liquidambar formosana Hance is a pinene-rich deciduous plant species in the Altingiaceae family that used as medicinal China. However, regulatory mechanisms underlying α-pinene and β-pinene biosynthesis L. leaves remain unknown. Here, joint analysis of volatile compounds transcriptomes was performed to comprehensively explore terpene synthase (TPS) may participate biosynthesis. Headspace solid-phase microextraction (HS-SPME) gas chromatography–mass spectrometry (GC–MS) jointly detected...
Abstract Tweety homologs (TTYHs) comprise a conserved family of transmembrane proteins found in eukaryotes with three members (TTYH1-3) vertebrates. They are widely expressed mammals including at high levels the nervous system and have been implicated cancers other diseases epilepsy, chronic pain, viral infections. TTYHs reported to form Ca 2+ - cell volume-regulated anion channels structurally distinct from any characterized protein potential roles adhesion, migration, developmental...
Abstract Metabolic reprogramming is a hallmark of cancer. Herein we discover that the key glycolytic enzyme pyruvate kinase M2 isoform (PKM2), but not related PKM1, methylated by co-activator-associated arginine methyltransferase 1 (CARM1). PKM2 methylation reversibly shifts balance metabolism from oxidative phosphorylation to aerobic glycolysis in breast cancer cells. Oxidative depends on mitochondrial calcium concentration, which becomes critical for cell survival when blocked. By...
On the basis of statistical analysis earthquake and storm disaster data, we report that quantities which are significantly correlated to economic level a country might not be number deaths losses, or their respective share in total population output, but ease degree making changes losses deaths. If D, F represent deaths, respectively, then parameter |D <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -D...
Summary TRAAK is a mechanosensitive two-pore domain K + (K2P) channel localized to nodes of Ranvier in myelinated neurons. deletion mice results mechanical and thermal allodynia gain-of-function mutations cause the human neurodevelopmental disorder FHEIG. displays basal stimulus-gated activities typical K2Ps, but mechanistic structural differences between these modes are unknown. Here, we demonstrate that mechanically-gated openings distinguished by their conductance, kinetics, structure....