Plant growth-promoting rhizobacteria (PGPR) increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium) apparently provides greater benefits to than single strain. In the present work, consortium was formulated containing four compatible desiccation-tolerant strains potential as PGPR. formulation had one moderately (Pseudomonas putida KT2440) three highly (Sphingomonas sp. OF178, Azospirillum brasilense Sp7 Acinetobacter EMM02) strains. were...

In Azotobacter vinelandii the two-component GacS/GacA system is required for synthesis of polyhydroxybutyrate (PHB) and exopolysaccharide alginate. The RsmA protein was shown to interact with alginate biosynthetic algD mRNA, acting as a translational repressor, GacA found activate transcription rsmZ1 rsmZ2 genes that encode small RNAs interacting counteract its repressor activity. phbBAC operon encodes enzymes PHB activated by transcriptional regulator PhbR. This study shows one rsmY seven...

The Pseudomonas aeruginosa genome can change to adapt different ecological niches. We compared four genomes from a Mexican hospital and 59 GenBank niches, such as urine, sputum, environmental. ST analysis showed that high-risk STs (ST235, ST773, ST27) were present in the of three niches GenBank, (ST167, ST2731, ST549) differed genomes. Phylogenetic clustering according their not niche. When analyzing genomic content, we observed environmental had genes involved adapting environment found...

Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and intracellular polyester poly-beta-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from an A. gene library diminished production by mucoid strain ATCC 9046. The nucleotide sequence predicted amino acid of locus responsible for mucoidy suppression revealed 65% identity to Pseudomonas GacS, a transmembrane sensor kinase two-component regulators, whose cognate response regulator, GacA, is global activator...

In Azotobacter vinelandii, a cyst-forming bacterium, the alternative sigma factor RpoS is essential to formation of cysts resistant desiccation and synthesis cyst-specific lipids, alkylresorcinols. this study, we carried out proteome analysis vegetative cells A. vinelandii strain AEIV its rpoS mutant derivative AErpoS. This allowed us identify small heat-shock protein, Hsp20, as one most abundant proteins regulated by RpoS. Inactivation hsp20 did not affect alkylresorcinols or with WT...

<i>Azotobacter vinelandii </i>is a soil bacterium that produces the polysaccharide alginate. The two-component system GacS/GacA is required for alginate synthesis since mutation in <i>gacS </i>or <i>gacA </i>significantly reduced level of transcripts <i>algD</i>, gene encoding GDP-mannose dehydrogenase, key enzyme biosynthetic pathway. In many γ-proteobacteria, GacA homologs control expression small regulatory RNAs RsmZ/Y/X (CsrB/CsrC) family...

ABSTRACT Transcription of the Azotobacter vinelandii algD gene, which encodes GDP-mannose dehydrogenase (the rate-limiting enzyme alginate synthesis), starts from three sites: p1, p2, and p3. The sensor kinase GacS, a member two-component regulatory system, is required for transcription its sites during stationary phase. Here we show that expressed constitutively throughout growth cycle p2 p3 p1 started at transition between exponential phase We constructed A . strains carried mutations in...

Methanol-consuming culturable bacteria were isolated from the plant surface, rhizosphere, and inside stem of Neobuxbaumia macrocephala. All 38 isolates facultative methylotrophic microorganisms. Their classification included Classes Actinobacteria, Sphingobacteriia, Alpha-, Beta-, Gammaproteobacteria. The deduced amino acid sequences methanol dehydrogenase obtained by PCR belonging to Gammaproteobacteria showed high similarity rare-earth element (REE)-dependent XoxF dehydrogenases,...

ABSTRACT During encystment of Azotobacter vinelandii , a family alkylresorcinols (ARs) and alkylpyrones (APs) are synthesized. In the mature cyst, these lipids replace membrane phospholipids also components layers covering cyst. this study, A. strains unable to synthesize ARs were isolated after mini-Tn 5 mutagenesis. Cloning nucleotide sequencing affected loci revealed presence transposons within arsA gene previously reported arsABCD cluster, which encodes type I fatty acid synthase. A...

Upon encystment induction, Azotobacter vinelandii produces the phenolic lipids alkylresorcinols (ARs) that are structural components of cysts. The enzymes responsible for ARs synthesis encoded in arsABCD operon, whose expression is activated by ArpR. transcription arpR initiated from an RpoS dependent promoter. nitrogen-related phosphotransferase system (PTSNtr) a global regulatory present Gram negative bacteria. It comprises EINtr, NPr and EIIANtr proteins ptsP, ptsO ptsN genes...

Multidrug-resistant bacteria are a growing problem in different environments and hosts, but scarce information exists about their prevalence reptiles. The aim of this study was to analyze the resistance mechanisms, molecular typing, plasmid content cefotaxime-resistant (CTX(R)) Escherichia coli isolates recovered from cloacal samples 71 turtles sheltered herpetarium Mexico. CTX(R)-E. were 11 (15.5%), one isolate/sample characterized. Extended-spectrum β-lactamase (ESBL)-producing E. detected...

Purpose: Pseudomonas aeruginosa infections in hospitals constitute an important problem due to the increasing multidrug resistance (MDR) and carbapenems resistance. The knowledge of mechanisms strains is issue for adequate antimicrobial treatment. Therefore, objective was investigate other MDR P. carrying bla IMP , make a partial plasmids characterization, determine if modifications oprD gene affect expression OprD protein. Methodology: Susceptibility testing performed by Kirby Baüer Minimum...

Azotobacter vinelandii is a soil gamma-proteobacteria that fixes nitrogen and forms desiccation-resistant cysts. The exopolysaccharide alginate an integral part of the layers surrounding Here, we reported cloning A. algC, encoding enzyme catalyzing second step pathway. We showed AlgC involved not only in production, but also lipopolysaccharide (LPS) synthesis it seems to have both phosphomannomutase phosphoglucomutase activities. transcriptional analysis algC gene contained two start sites,...

Azotobacter vinelandii is a soil bacterium that undergoes differentiation to form cysts are resistant desiccation. Upon induction of cyst formation, the synthesizes alkylresorcinols present in but not vegetative cells. Alternative sigma factors play important roles differentiation. In A. vinelandii, AlgU (sigma E) involved controlling loss flagella upon encystment. We investigated involvement factor RpoS formation vinelandii. analysed transcriptional regulation rpoS gene by PsrA, main...

Azotobacter vinelandii is a soil bacterium that undergoes differentiation process forms cysts resistant to desiccation. During encystment, family of alkylresorcinols lipids (ARs) are synthesized and become part the membrane also components outer layer covering cyst, where they play structural role. The synthesis ARs in A. has been shown occur by activity enzymes encoded arsABCD operon. expression this operon activated ArpR, LysR-type transcriptional regulator whose transcription occurs...

Azotobacter vinelandii, belonging to the Pseudomonadaceae family, is a free-living bacterium that has been considered be good source for production of bacterial polymers such as alginate. In A. vinelandii synthesis this polymer regulated by Gac/Rsm post-transcriptional regulatory system, in which RsmA protein binds mRNA biosynthetic algD gene, inhibiting translation. several Pseudomonas spp. two-component system CbrA/CbrB described control variety metabolic and behavioural traits needed...

The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered be non-motile non-flagellated. In present work, 25 strains isolated from nosocomial infections were assessed for motility under different growth conditions. One isolate, KpBUAP021, demonstrated a swim-like phenotype. K. pneumoniae genotype was confirmed by 16S rRNA rpoB gene sequence analysis. Multilocus typing analysis also revealed that KpBUAP021 strain places it in ST345 type, phylogenetic Kpl...

Acinetobacter calcoaceticus-baumannii complex isolates have been frequently associated with hospital and community infections, A. baumannii being the most common. Other spp. not belonging to this also cause infections in settings, incidence has increased over past few years. Some species of genus possess a great diversity antibiotic resistance mechanisms, such as efflux pumps, porins, genes that can be acquired disseminated by mobilizable genetic elements. By means whole-genome sequencing,...