A5024572762- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Microbial bioremediation and biosurfactants
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacterial Identification and Susceptibility Testing
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Ocular Infections and Treatments
- Methane Hydrates and Related Phenomena
- Yersinia bacterium, plague, ectoparasites research
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Brucella: diagnosis, epidemiology, treatment
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Nematode management and characterization studies
- Phytase and its Applications
- Cellular transport and secretion
- Salmonella and Campylobacter epidemiology
- Plant Growth Enhancement Techniques
- Gut microbiota and health
Universidad Nacional Autónoma de México
2014-2025
Universidad Autónoma del Estado de Morelos
2020-2025
Ornithine lipids (OLs) are widespread among Gram-negative bacteria. Their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino ornithine and second ester-linked position first acid. OLs can be hydroxylated within secondary moiety this modification has been related increased stress tolerance. Rhizobium tropici, nodule-forming α-proteobacterium known for its tolerance, forms four different OLs. Studies function these have hampered due lack knowledge...
In Proteobacteria, the outer membrane protein TamA and inner membrane-anchored TamB form Translocation Assembly Module (TAM) complex, which facilitates transport of autotransporters, virulence factors, likely lipids across two membranes. Bacteroidota, is replaced by TamL, a TamA-like lipoprotein with lipid modification at its N-terminus that anchors it to membrane. This structural difference suggests TamL may have distinct function compared TamA. However, role TAM in bacterial phyla other...
Ornithine lipids (OLs) are phosphorus-free membrane that can be formed by many bacteria but absent from archaea and eukaryotes. A function for OLs in stress conditions host-bacteria interactions has been shown some bacteria. Some bacterial species have described form OLs, lack the known genes (olsBA) involved its biosynthesis, which implied existence of a second pathway. Here we describe bifunctional protein OlsF Serratia proteamaculans OL formation. Expression homologue Flavobacterium...
Ornithine lipids (OLs) are phosphorus-free membrane that widespread among Gram-negative bacteria. Their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino ornithine and second ester-linked position first acid. It has been shown OLs can be hydroxylated within amide-linked moiety, secondary moiety or moiety. These modifications have related increased stress tolerance symbiotic proficiency different organisms such as Rhizobium tropici Burkholderia...
Azotobacter vinelandii is a soil bacterium that undergoes differentiation process forms cysts resistant to desiccation. During encystment, family of alkylresorcinols lipids (ARs) are synthesized and become part the membrane also components outer layer covering cyst, where they play structural role. The synthesis ARs in A. has been shown occur by activity enzymes encoded arsABCD operon. expression this operon activated ArpR, LysR-type transcriptional regulator whose transcription occurs...
The genus Burkholderia sensu lato is composed of a diverse and metabolically versatile group bacterial species. One characteristic thought to be unique for the presence two forms each (with without 2-hydroxylation) membrane lipids phosphatidylethanolamine (PE) ornithine (OLs). Here, we show that only stricto strains constitutively form OLs, whereas all other analyzed belonging PE, but no OLs. We selected model bacteria study function OL in lato: (1) cenocepacia wild-type which OLs its mutant...
ABSTRACT Sinorhizobium meliloti contains phosphatidylglycerol, cardiolipin, phosphatidylcholine, and phosphatidylethanolamine (PE) as major membrane lipids. PE is formed in two steps. In the first step, phosphatidylserine synthase (Pss) condenses serine with CDP-diglyceride to form (PS), second PS decarboxylated by decarboxylase (Psd) PE. this study we identified sinorhizobial psd gene coding for Psd. A mutant deficient unable but accumulates anionic phospholipid PS. Properties of...
Abstract Persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs) are a group of high-risk synthetic substances for human and environmental health. Currently, the study sites contaminated by spillage equipment PCBs containing have been considered targeted areas bacterial communities with potential degradation. There in isolation strains is vital use biodegradable processes, bioaugmentation, which accelerates development phenomena natural attenuation sites. The objective...
Summary Sulfonolipids (SLs) are bacterial lipids that structurally related to sphingolipids. Synthesis of this group seems be mainly restricted Flavobacterium , Cytophaga and other members the phylum Bacteroidetes . These have a wide range biological activities: they can induce multicellularity in choanoflagellates, act as von Willebrand factor receptor antagonists, inhibit DNA polymerase, or function tumour suppressing agents. In johnsoniae their presence required for efficient gliding...
The brucellae are facultative intracellular bacteria with a cell envelope rich in phosphatidylcholine (PC). PC is abundant eukaryotes but rare prokaryotes, and it has been proposed that Brucella uses to mimic eukaryotic-like features avoid innate immune responses the host. Two synthesis pathways known prokaryotes: PmtA-catalyzed trimethylation of phosphatidylethanolamine direct linkage choline CDP-diacylglycerol catalyzed by synthase Pcs. Previous studies have reported B. abortus melitensis...
Summary Treponema denticola synthesizes phosphatidylcholine through a licCA ‐dependent CDP‐choline pathway identified only in the genus . However, mechanism of conversion to remained unclear. We report here characterization TDE0021 (herein designated cpt ) encoding 1,2‐diacylglycerol choline phosphotransferase homologous phosphotransferases that catalyze final step highly conserved Kennedy for synthesis eukaryotes. T. Cpt catalyzed vitro formation from and diacylglycerol, full activity...
The brucellae are facultative intracellular bacteria that cause a worldwide extended zoonosis. One of the pathogenicity mechanisms these is their ability to avoid rapid recognition by innate immunity because reduction pathogen-associated molecular pattern (PAMP) lipopolysaccharide (LPS), free-lipids, and other envelope molecules. We investigated Brucella homologs lptA, lpxE, lpxO, three genes in some pathogens encode enzymes mask LPS PAMP upsetting core-lipid A charge/hydrophobic balance....
Plant food production is affected by multiple abiotic and biotic stresses. To overcome these limiting factors, synthetic agrochemicals are widely used. However, more stringent worldwide regulatory policies the public demand to reduce their application have led search for new ecofriendly alternatives such as biostimulants. Their commercial use still limited, mostly due lack of knowledge molecular mechanisms responsible effects on plant metabolism. The horseradish Moringa oleifera has been...
Infectious endophthalmitis is a severe ophthalmic emergency. It known that this infection can be caused by bacteria and fungi. For efficient treatment, the administration of antimicrobial drugs to which microbes are susceptible essential. The aim study was identify microorganisms in biopsies Mexican patients using metagenomic next-generation sequencing determine antibiotic resistance genes were present biopsy samples. In prospective case study, 19 recruited. Samples vitreous or aqueous humor...
Infectious endophthalmitis is a severe ophthalmic emergency. This infection can be caused by bacteria and fungi. For efficient treatment, the administration of antimicrobial drugs to which microbes are susceptible essential. The aim this study was identify micro-organisms in biopsies Mexican patients using metagenomic next-generation sequencing determine antibiotic resistance genes were present biopsy samples. In prospective case study, 19 recruited. Samples vitreous or aqueous humour...
Abstract In Proteobacteria, the outer membrane protein TamA and inner membrane-anchored TamB form Translocation Assembly Module (TAM) complex, which facilitates transport of autotransporters, virulence factors, likely lipids across two membranes. Bacteroidetes is replaced by TamL, a TamA-like lipoprotein with lipid modification at its N-terminus that anchors it to membrane. This structural difference suggests TamL may have distinct function compared TamA. However, role TAM in bacterial phyla...
ABSTRACT Ornithine lipids (OLs) are phosphorus-free membrane that can be formed by a wide range of bacteria. The presence OLs is frequently related to the resistance abiotic stress conditions, and its synthesis often induced as part various responses. Two different pathways for synthesizing currently known: OlsBA pathway first described in Sinorhizobium meliloti , OlsF Serratia proteamaculans . We identified genome Vibrio cholerae O1 El Tor A1552 two genes encoding homologs, VC0489 located...