Ornithine lipids (OLs) are phosphorus-free membrane that can be formed by many bacteria but absent from archaea and eukaryotes. A function for OLs in stress conditions host-bacteria interactions has been shown some bacteria. Some bacterial species have described form OLs, lack the known genes (olsBA) involved its biosynthesis, which implied existence of a second pathway. Here we describe bifunctional protein OlsF Serratia proteamaculans OL formation. Expression homologue Flavobacterium...

Physiological response to feeding is crucial for various production factors as feed catabolism and growth. Despite growing significance in red Octopus maya aquaculture, large-scale commercial limited by not sufficiently knowing its nutritional needs, especially digestive physiology. Since this species carnivorous, one of the main aspects directed protein digestion, but enzymatic repertoire has been studied yet at genomic transcriptomic levels. This study searched protease enzymes encoded O....

Biofouling poses a significant economic threat to various marine industries, leading financial losses that can reach billions of euros annually. This study highlights the urgent need for effective alternatives traditional antifouling agents, particularly following global ban on organotin compounds.

Water-filled sinkholes known locally as cenotes, found on the Yucatán Peninsula, have remarkable biodiversity. The primary objective of this study was to explore biotechnological potential Gram-positive cultivable bacteria obtained from sediment samples collected at coastal cenote Pol-Ac in Yucatán, Mexico. Specifically, investigation aimed assess production hydrolytic enzymes and antimicrobial compounds. 16 S rRNA gene sequencing led identification 49 bacterial isolates belonging phyla...

Little is known about the diversity of bacteria in Southwestern Gulf Mexico. The aim study illustrated this perspective was to search for presence bacterial pathogens ecosystem, using metagenomic data recently generated by Mexican research group as Mexico Research Consortium. Several genera annotated were detected water and sediment marine samples. As expected, native ubiquitous pathogenic such Burkolderia, Halomonas, Pseudomonas, Shewanella Vibrio highly represented. Surprisingly,...

Abstract Toluene dioxygenase (TDO) from Pseudomonas putida F1 was engineered towards the oxyfunctionalization of bicyclic substrates. Single and double mutant libraries addressing 27 different positions, located at active site entrance channel were generated. In total, 176 variants tested employing substrates naphthalene, 1,2,3,4‐tetrahydroquinoline, 2‐phenylpyridine. Introduced mutations in positions M220, A223 F366, exhibited major influences terms product formation, chemo‐, regio‐...

Gastric ulcers are a worldwide health problem and their poor healing is one of the most important causes for recurrence. We have previously reported remarkable gastroprotective anti-Helicobacter pylori activities methanolic extract (CpMet) Cyrtocarpa procera bark. This work investigates, in murine model, CpMet mechanism establishes its preclinical efficacy resolution ethanol-induced gastric ulcers. The results showed that activity mainly associated with endogenous NO prostaglandins, followed...

As the demand for Octopus maya grows, sustainable farming practices become essential to prevent overexploitation, so that can be developed as a alternative traditional fishing. Understanding digestive dynamics of octopus is devising optimal dietary formulations in aquaculture. Despite progress understanding cephalopod digestion, little known about specific functioning enzymes responsible breaking down protein substrates. This knowledge gap underscores need further research support O....

Abstract Phenylobacterium immobile strain E is a soil bacterium with striking metabolism relying on xenobiotics, such as the herbicide pyrazon, sole carbon source instead of more bioavailable molecules. Pyrazon heterocyclic aromatic compound environmental concern and its biodegradation pathway has only been reported in P. . The multicomponent pyrazon oxygenase (PPO), Rieske non-heme iron oxygenase, incorporates molecular oxygen at 2,3 position phenyl moiety first step degradation, generating...

We aimed to expand the microbial biocatalyst platform generate essential oxyfunctionalized standards for pharmaceutical, toxicological and environmental research. In particular, we examined production of nonsteroidal anti-inflammatory drugs (NSAIDs) by filamentous-fungi.Four NSAIDs; diclofenac, ibuprofen, naproxen mefenamic acid were used as substrates oxyfunctionalization in a biocatalytic process involving three filamentous-fungi strains; Beauveria bassiana, Clitocybe nebularis Mucor...

Phosphorous-NMR is scarcely employed to evaluate enzyme kinetics of kinase driven monophosphorylations, despite being a powerful and reliable tool undoubtedly detect the actual phosphoryl transfer targeted substrate. Another advantage that an external supplementation source NMR active isotope not required, since 31P highly abundant in nature. Glycerol (GlpK) from E. coli exemplary ATP-dependent kinase/phosphotransferase model illustrate value usefulness 31P-NMR-based approach assess...

<title>Abstract</title> Water-filled sinkholes known locally as cenotes, found on the Yucatán Peninsula, have remarkable biodiversity. The primary objective of this study was to explore biotechnological potential Gram-positive cultivable bacteria obtained from sediment samples collected at coastal cenote Pol-Ac in Yucatán, Mexico. Specifically, investigation aimed assess production hydrolytic enzymes and antimicrobial compounds. 16S rRNA gene sequencing led identification 49 bacterial...

Abstract Background As the demand for Octopus maya grows, sustainable farming practices become essential to prevent overexploitation. Thus, its development can be a alternative traditional fishing. Understanding digestive dynamics is devising optimal dietary formulations in aquaculture, particularly role of enzymes like cathepsins and others. Despite progress understanding cephalopod digestion, little known about specific functioning responsible breaking down protein substrates. This...

ABSTRACT Ornithine lipids (OLs) are phosphorus-free membrane that can be formed by a wide range of bacteria. The presence OLs is frequently related to the resistance abiotic stress conditions, and its synthesis often induced as part various responses. Two different pathways for synthesizing currently known: OlsBA pathway first described in Sinorhizobium meliloti , OlsF Serratia proteamaculans . We identified genome Vibrio cholerae O1 El Tor A1552 two genes encoding homologs, VC0489 located...

cis-Dihydrodiendiols are valuable compounds, finding multiple application as chiral synthons in organic chemistry. The biotechnological route for the generation of cis-dihydrodiendiols involves dihydroxylation aromatic catalyzed by Rieske non-heme iron dioxygenases. To date, numerous examples recombinant E. coli, harboring such dioxygenases, can be found literature. Nevertheless, there is only a minor number publications, addressing coli degradation into catechols via dehydrogenases....

In an attempt to establish a biosynthetic route towards isobutene, we faced the problem that first intermediate isobutyl-monophosphate was not commercially available. order overcome this limitation searched in literature for protocols reporting synthesis of phosphate monoesters from alcohols. Based on suitability preceding developments our purposes, established customized method fast, easy and affordable generation pursued molecule. Herein, prompt straightforward (ammonium salt) is provided....