A5018097949- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Connective tissue disorders research
- Carbohydrate Chemistry and Synthesis
- Fibroblast Growth Factor Research
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Genomics and Rare Diseases
- Platelet Disorders and Treatments
- Peptidase Inhibition and Analysis
- Neurogenetic and Muscular Disorders Research
- Polysaccharides Composition and Applications
- Polysaccharides and Plant Cell Walls
- Pediatric Hepatobiliary Diseases and Treatments
- Veterinary Equine Medical Research
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Osteoarthritis Treatment and Mechanisms
- Digestive system and related health
- Hydrogels: synthesis, properties, applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- Protein Tyrosine Phosphatases
- Silk-based biomaterials and applications
Meijo University
2016-2025
University of Otago
2018-2020
Hokkaido University
2007-2018
Zero to Three
2010
Kobe Pharmaceutical University
2003-2009
Nara Medical University
1993
Based on the molecular stent concept, a series of tough double-network hydrogels (St-DN gels) made from components proteoglycan aggregates – chondroitin sulfate proteoglycans (1), (2), and sodium hyaluronate (3) are successfully developed in combination with neutral biocompatible polymer. This work demonstrates promising method to create biopolymer-based for biomedical applications.
Ehlers-Danlos syndrome (EDS) is a heterogeneous connective tissue disorder involving skin and joint laxity fragility. A new type of EDS, similar to kyphoscoliosis but without lysyl hydroxylase deficiency, has been investigated. We have identified homozygous CHST14 (carbohydrate sulfotransferase 14) mutation in the two familial cases compound heterozygous mutations four sporadic cases. encodes dermatan 4-O-sulfotransferase 1 (D4ST1), which transfers active sulfate from 3'-phosphoadenosine...
Heparanase acts as a master regulator of the aggressive tumor phenotype in part by enhancing expression proteins known to drive progression (e.g. VEGF, MMP-9, hepatocyte growth factor (HGF), and RANKL). However, mechanism whereby this enzyme regulates gene remains unknown. We previously reported that elevation heparanase levels myeloma cells causes dramatic reduction amount syndecan-1 nucleus. Because has heparan sulfate chains because exogenous been shown inhibit activity histone...
Human hyaluronidases have been considered to be the enzymes acting at initial step in catabolism of chondroitin sulfate (CS) vivo. However, human hyaluronidase-1 digests CS more slowly than hyaluronan (HA), and its preferred substrate is HA rather CS. We identified a hydrolase Caenorhabditis elegans, which effectively degrades but depolymerizes much lesser extent (Kaneiwa T, Yamada S, Mizumoto Montaño AM, Mitani Sugahara K. 2008. Identification novel elegans. J Biol Chem. 283:14971–14979),...
Altered expression of chondroitin sulfate (CS) and heparan (HS) at the surfaces tumor cells plays a key role in malignant transformation metastasis. Previously we demonstrated that Lewis lung carcinoma (LLC)-derived cell line with high metastatic potential had higher proportion E-disaccharide units, GlcUA-GalNAc(4,6-O-disulfate), CS chains than low LLC such are involved process. The metastasis was markedly inhibited by pre-administration CS-E from squid cartilage rich E units or...
Early-onset epileptic encephalopathies (EOEE) are severe neurological disorders characterized by frequent seizures accompanied developmental regression or retardation. Whole-exome sequencing of 12 patients together with five pairs parents and subsequent Sanger in additional 328 EOEE identified two de novo frameshift one missense mutations SLC35A2 at Xp11.23, respectively. The three all females. X-inactivation analysis blood leukocyte DNA mRNA using lymphoblastoid cells derived from a...
The sulfated polysaccharide dermatan sulfate (DS) forms proteoglycans with a number of distinct core proteins. Iduronic acid-containing domains in DS have key role mediating the functions proteoglycans. Two tissue-specific epimerases, encoded by DSE and DSEL, GalNAc-4-O-sulfotransferase CHST14 are necessary for formation these domains. mutations were previously identified patients musculocontractural type Ehlers–Danlos syndrome (MCEDS). We now homozygous missense mutation (c.803C>T, p.S268L)...
From 1979 to 1990 we treated 20 patients with large bone defects or established nonunion of the femur by vascularised fibular grafts. There were 18 men and two women an average age at operation 36.6 years (16 69). Ten had infected nonunion, three post-traumatic a defect without infection, four after tumour resection, other lesions. The mean length grafts was 18.1 cm. Postoperative circulatory disturbances needed revision surgery in five patients, including problems monitoring flap, but not...
Chondroitin sulfate (CS) and dermatan (DS) have been implicated in the processes of neural development brain. In this study, we characterized developmentally regulated brain CS/DS chains using a single chain antibody, GD3G7, produced by phage display technique. Evaluation specificity GD3G7 toward various glycosaminoglycan preparations showed that antibody specifically reacted with squid CS-E (rich GlcUAβ1–3GalNAc(4,6-O-sulfate) disaccharide unit E), hagfish CS-H...
Chondroitin sulfate (CS) chains are involved in the regulation of various biological processes. However, mechanism underlying catabolism CS is not well understood. Hyaluronan (HA)-degrading enzymes, hyaluronidases, assumed to act at initial stage degradation process, because HA similar structure nonsulfated CS, chondroitin (Chn). Although human hyaluronidase-1 (HYAL1) and testicular hyaluronidase (SPAM1) can degrade only but also they digest a limited extent. In this study, hydrolytic...
Wnt proteins direct embryonic patterning, but the regulatory basis of their distribution and signal reception remain unclear. Here, we show that endogenous Wnt8 protein is distributed in a graded manner Xenopus embryo accumulated on cell surface punctate association with "N-sulfo-rich heparan sulfate (HS)," not "N-acetyl-rich HS". These two types HS are differentially clustered by attaching to different glypicans as core proteins. N-sulfo-rich frequently internalized associated signaling...
Induction of mucosal healing (MH) is an important treatment goal in inflammatory bowel disease (IBD). Although the molecular mechanisms underlying MH IBD not fully explored, local fibrosis would contribute to interfere repair. Carbohydrate sulfotransferase 15 (CHST15), which catalyzes sulfation chondroitin sulfate produce rare E-disaccharide units, a novel mediator create fibrosis. Here we have used siRNA-based approach silencing CHST15 dextran sodium (DSS) induced colitis mice, human colon...