A5082754982- Molecular Sensors and Ion Detection
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Cancer, Hypoxia, and Metabolism
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- Luminescence and Fluorescent Materials
- Obstructive Sleep Apnea Research
- Cancer-related molecular mechanisms research
- Calcium signaling and nucleotide metabolism
- Supramolecular Chemistry and Complexes
- Salivary Gland Disorders and Functions
- Analytical Chemistry and Sensors
- Advanced Glycation End Products research
- Retinal Diseases and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Adenosine and Purinergic Signaling
- Photochemistry and Electron Transfer Studies
- Epigenetics and DNA Methylation
- Neurological Disease Mechanisms and Treatments
- Pancreatitis Pathology and Treatment
- High Altitude and Hypoxia
- Digestive system and related health
- Genetics and Neurodevelopmental Disorders
- Electrochemical sensors and biosensors
Nara Medical University
2011-2023
Nara Medical University Hospital
2012
Tohoku University
1997-2008
University of Notre Dame
2001
Center for Biologics Evaluation and Research
1996
A novel boronic acid fluorophore 1/beta-cyclodextrin (beta-CyD) complex sensor for sugar recognition in water has been designed. The probe 1 bearing pyrene moiety as a fluorescent signal transducer exhibits no fluorescence emission, due to its aggregation containing 2% DMSO; however, the addition of beta-CyD this solution largely changes UV-vis and spectra by forming an inclusion with beta-CyD, efficient emission response 1/beta-CyD upon binding is found be obtained at pH 7.5....
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTBenzo-15-crown-5 Fluoroionophore/γ-Cyclodextrin Complex with Remarkably High Potassium Ion Sensitivity and Selectivity in WaterAkiyo Yamauchi, Takashi Hayashita, Seiichi Nishizawa, Masatoshi Watanabe, Norio TeramaeView Author Information Department of Chemistry, Graduate School Science Tohoku University, Aoba-ku, Sendai 980-8578, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 10, 2319–2320Publication Date (Web):February 24, 1999Publication...
The phenylboronic acid azoprobe (BA-Azo)/gamma-cyclodextrin (gamma-CD) complex exhibits a selective response for D-glucose by forming supramolecular 2:1 inclusion of the azoprobes with inside gamma-CD cavity.
Sleep apnea syndrome (SAS), characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), is a risk factor for insulin resistance. Recently, IH considered to independently cause adipose tissue inflammation/dysfunction, leading worsening resistance; however, the detailed mechanism remains unknown. We exposed mouse 3T3-L1 human SW872 adipocytes experimental or normoxia 24 h, analyzed mRNA expression several adipokines. found that levels RETN, TNFα,...
Three fluoroionophores Cn (n = 1, 3, 5), in which the crown ether unit and pyrenyl moiety are connected by a -(CH2)n- spacer, have been used to construct supramolecular Cn/gamma-cyclodextrin (gamma-CyD) complexes for alkali metal ion sensing water. The 5) found selectively form 2:1 with K+ presence of gamma-CyD exhibit pyrene dimer emission Equilibrium analysis C3/gamma-CyD complex reveals that observed arises from 2:1:1 C3 gamma-CyD. In absence K+, fluorescence lifetimes species ((C3)2CyD...
IL-2-, IL-12-, and IFN-alpha-mediated signaling pathways were analyzed in primary NK cells the NK3.3 cell line. Gel mobility shift immunoprecipitation analyses revealed that addition to activating STAT3 (signal transducer activator of transcription-3) STAT5, IL-2 induced tyrosine serine phosphorylation STAT1 alpha, which formed IFN-gamma-activated sequence-binding complexes by itself with STAT3. Although IFN-alpha activated alpha predominantly while alpha. less activation greater STAT5...
Regenerating gene product (Reg) is induced in pancreatic beta-cells and acts as an autocrine/paracrine growth factor for regeneration via a cell surface Reg receptor. However, the manner by which induces beta-cell was unknown. In present study, we found that increased phospho-ATF-2, binds to -57 -52 of cyclin D1 activate promoter. The Reg/ATF-2-induced promoter activation attenuated PI(3)K inhibitors such LY294002 wortmannin. knockout mouse islets, levels D1, phospho-Rb were greatly...
The pathophysiology of inflammatory bowel disease (IBD) reflects a balance between mucosal injury and reparative mechanisms. Some regenerating gene (Reg) family members have been reported to be expressed in Crohn's (CD) ulcerative colitis (UC) involved as proliferative factors IBD. However, expression all REG genes IBD is still unclear. Here, we analyzed (REG Iα, Iβ, III, HIP/PAP, IV) biopsy specimens UC CD by real-time RT-PCR. IV were overexpressed samples. was also We further the...
Highly selective HCO3- sensing is realized in a pH 8.6 aqueous solution on the basis of HCO3--induced anomalous pyrene fluorescence an association dimer that consists pyrene-appended gamma-cyclodextrins having triamine linker. The produced by twisted conformation residues insensitive to presence other anions.
Sleep apnea syndrome is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]) a risk factor for insulin resistance/type 2 diabetes. However, the mechanisms linking IH stress resistance remain elusive. We exposed human hepatocytes (JHH5, JHH7, HepG2) to experimental or normoxia 24 h, measured mRNA levels real-time reverse transcription polymerase chain reaction (RT-PCR), found that significantly increased selenoprotein P (SELENOP) —...
Reg ( Regenerating gene ) was originally isolated from rat regenerating islets and its encoding protein revealed as an autocrine/paracrine growth factor for β cells. Rat is activated in inflammatory conditions cell regeneration. In human, although five functional REG family genes I α , III, HIP/PAP IV were isolated, their expressions cells under remained unclear. this study, we found that combined addition of IL-6 dexamethasone (Dx) induced expression human 1.1B4 Promoter assay a signal...
Although recent research showed that advanced glycation endproduct (AGE) and hydroquinone (HQ) are related to the pathogenesis of age-related macular degeneration (AMD), mechanism how AGE HQ induce or accelerate AMD remains elusive. In present study, we examined effects on changes human retinal pigment epithelial (RPE) cell numbers found viable were markedly reduced by apoptosis prevented decreases HQ-treated increased replicative DNA synthesis RPE cells without changing apoptosis. Real-time...
Abstract Background Regenerating gene (Reg) product, Reg, acts as an autocrine/paracrine growth factor for beta‐cell regeneration. The presence of autoimmunity against REG may affect the operative regenerative mechanisms in beta cells Type 1 and 2 diabetes patients. We screened sera from subjects anti‐REG autoantibodies, searched correlations general characteristics with autoimmunity, tested attenuation REG‐induced proliferation by autoanitibodies. Material methods examined occurrence...
The regenerating gene (Reg) was isolated originally as a specifically over-expressed in pancreatic islets and constitute growth factor family. Reg product is important the pathophysiology of various human inflammatory diseases. Recently, possible involvement REG regeneration salivary ductal epithelial cells patients with primary Sjögren's syndrome (SS) reported. However, expression family genes minor glands (MSG) occurrence anti-REG Iα autoantibodies SS were obscured. In this study, we...
The patients with sleep apnea syndrome are exposed to intermittent hypoxia (IH) during sleep. We previously demonstrated the IH-induced up-regulation of mRNA levels anorexigenic peptides proopiomelanocortin (POMC), and cocaine- amphetamine-regulated transcript (CART) in human neuronal cells. Appetite is regulated not only by central nervous system but also from gastrointestinal tract. Here, we investigated effects IH on gene expression(s) appetite-inhibiting gut hormones. Human...
Preeclampsia/hypertensive disorders of pregnancy (PE/HDP) is a serious and potentially life-threatening disease. Recently, PE/HDP has been considered to cause adipose tissue inflammation, but the detailed mechanism remains unknown. We exposed human primary cultured adipocytes with serum from healthy controls for 24 h, analyzed mRNA expression several adipokines, cytokines, ligands receptor advanced glycation endproducts (RAGE). found that levels interleukin-6 (IL-6), C-C motif chemokine...