A5015278458- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
Hebrew University of Jerusalem
2020-2022
Hadassah Medical Center
2020
In the last decade, multiple studies demonstrated that cells maintain a balance of mRNA production and degradation, but mechanisms by which implement this remain unknown. Here, we monitored cells' total recently-transcribed profiles immediately following an acute depletion Xrn1-the main 5'-3' exonuclease-which was previously implicated in balancing levels. We captured detailed dynamics adaptation to rapid degradation Xrn1 observed significant accumulation mRNA, followed delayed global...
A sequencing-based detection and genotyping assay for SARS-CoV-2 is based on early sample pooling using barcoded oligo hybridization.
Abstract The global SARS-CoV-2 pandemic created a dire need for viral detection tests worldwide. Most current are based on RNA extraction followed by quantitative reverse-transcription PCR assays. While automation and improved logistics increased the capacity of these tests, they cannot exceed lower bound dictated one RT-PCR reaction per sample. Multiplexed next generation sequencing (NGS) assays provide dramatic increase in throughput, hold promise richer information including strains, host...
The global SARS-CoV-2 pandemic led to a steep increase in the need for viral detection tests worldwide. Most current are based on RNA extraction followed by quantitative reverse-transcription PCR assays that involve separate and qPCR reaction each sample with fixed cost time. While automation improved logistics can capacity of these tests, they cannot exceed this lower bound dictated one per sample. Multiplexed next generation sequencing (NGS) provide dramatic throughput, hold promise richer...
The global SARS-CoV-2 pandemic led to a steep increase in the need for viral detection tests worldwide. Most current are based on RNA extraction followed by quantitative reverse-transcription PCR assays that involve separate and qPCR reaction each sample with fixed cost time. While automation improved logistics can capacity of these tests, they cannot exceed this lower bound dictated one per sample. Multiplexed next generation sequencing (NGS) provide dramatic throughput, hold promise richer...
Abstract In the last decade, multiple studies have shown that cells maintain a balance of mRNA production and degradation, but mechanisms by which implement this remain unknown. Here, we monitored cells’ nascent profiles immediately following an acute depletion Xrn1 - main 5’-3’ exonuclease was previously implicated in balancing levels. We captured detailed dynamics adaptation to rapid degradation observed significant accumulation mRNA, followed delayed global reduction transcription gradual...
The global SARS-CoV-2 pandemic led to a steep increase in the need for viral detection tests worldwide. Most current are based on RNA extraction followed by quantitative reverse-transcription PCR assays that involve separate and qPCR reaction each sample with fixed cost time. While automation improved logistics can capacity of these tests, they cannot exceed this lower bound dictated one per sample. Multiplexed next generation sequencing (NGS) provide dramatic throughput, hold promise richer...
The global SARS-CoV-2 pandemic led to a steep increase in the need for viral detection tests worldwide. Most current are based on RNA extraction followed by quantitative reverse-transcription PCR assays that involve separate and qPCR reaction each sample with fixed cost time. While automation improved logistics can capacity of these tests, they cannot exceed this lower bound dictated one per sample. Multiplexed next generation sequencing (NGS) provide dramatic throughput, hold promise richer...