A5065261917- Antibiotic Resistance in Bacteria
- Genetically Modified Organisms Research
- Bacteriophages and microbial interactions
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- DNA and Biological Computing
- Plant Pathogens and Resistance
- Chemical Synthesis and Analysis
- Bacterial Genetics and Biotechnology
- Insect Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Genomic variations and chromosomal abnormalities
- Biochemical and Structural Characterization
- Gene expression and cancer classification
- Enzyme Production and Characterization
- Escherichia coli research studies
- Intellectual Property and Patents
- Streptococcal Infections and Treatments
- Potato Plant Research
- Antimicrobial Resistance in Staphylococcus
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Neonatal and Maternal Infections
- Microbial Natural Products and Biosynthesis
- Animal Genetics and Reproduction
Simplot (United States)
2020-2024
Plant (United States)
2024
Baylor College of Medicine
1996-2001
Autonomous chromosomes are generated in yeast (yeast artificial chromosomes) and human fibrosarcoma cells (human by introducing purified DNA fragments that nucleate a kinetochore, replicate, segregate to daughter cells. These autonomous minichromosomes convenient for manipulating delivering segments containing multiple genes. In contrast, commercial production of transgenic crops relies on methods integrate one or few genes into host chromosomes; extensive screening identify insertions with...
β-Lactamases are bacterial enzymes that hydrolyze β-lactam antibiotics to render them inactive. The β-lactamase inhibitor protein (BLIP) of <i>Streptomyces clavuligerus</i>, is a potent several β-lactamases, including the TEM-1 enzyme (<i>K</i> <sub>i</sub> = 0.6 nm). Evidence from TEM-1/BLIP co-crystal suggests two BLIP residues, Asp-49 and Phe-142, mimic interactions made by penicillin G when bound in active site TEM-1. To determine importance these heterologous expression system for was...
Virtually all strains of the human pathogenic bacterium Streptococcus pyogenes express a highly conserved extracellular cysteine protease. The protein is made as an inactive zymogen 40,000 Da and undergoes autocatalytic truncation to result in 28,000-Da active Numerous independent lines investigation suggest that this enzyme participates one or more phases host-parasite interaction, such inflammation soft tissue invasion. Replacement single residue (C-192) with serine (C192S mutation)...
ABSTRACT To overcome the antibiotic resistance mechanism mediated by β-lactamases, small-molecule β-lactamase inhibitors, such as clavulanic acid, have been used. This approach, however, has applied selective pressure for mutations that result in β-lactamases no longer sensitive to inhibitors. On basis of structure inhibitor protein (BLIP), novel peptide inhibitors constructed. BLIP is a 165-amino-acid potent TEM-1 ( K i = 0.3 nM). The cocrystal and indicates residues 46 51 make critical...
DAS-81910-7 cotton is a transgenic event that was transformed to contain the aad-12 and pat genes. These genes code for AAD-12 PAT proteins, which confer tolerance herbicides 2,4-D glufosinate, respectively. Crop composition studies were conducted with (both nonsprayed sprayed glufosinate) comply requirements of regulatory authorities responsible evaluating crop safety. Results indicate compositional equivalence between cottonseed nontransgenic cottonseed. This study builds on results from...
Protein–protein interactions are involved in most biological processes and important targets for drug design. Over the past decade, there has been increased interest design of small molecules that mimic functional epitopes protein inhibitors. BLIP is a 165 amino acid potent inhibitor TEM-1 β-lactamase (Ki = 0.1 nM). To aid development new inhibitors β-lactamase, gene encoding was randomly fragmented DNA segments peptides retain ability to bind were isolated using phage display. The selected...
Small procedural changes in how regulatory agencies implement biotech policies can make significant differences improving efficiency. This paper discusses science based, crop specific guidance documents improve dossier content and the review approval of varieties. In addition, we describe adoption established risk assessment methodology applying policy-linked decision making at agency level boost both efficiency developer, public government confidence crops.
Regulatory agencies require data on genetic stability as part of the safety assessment for biotech crops, even though a plant is not necessarily an environmental, human or animal health concern. While sexual reproduction has potential to introduce genomic variation in conventionally bred and vegetative propagation genetically stable. In vegetatively propagated meiosis does occur thus limiting number homologous recombination events that could lead chromosomal rearrangements progeny plants....
AbstractProtein-protein interactions are involved in most biological processes and an important target for drug design. Over the past decade, there has been increased interest design of small molecules that mimic functional epitopes protein-protein interactions. However, disrupt remains a considerable challenge (1,2). Progress achieved towards minimizing proteins into significantly smaller polypeptides retain ability to bind partner protein (3–5). These mini-proteins represent potential...