Abstract The study of green analytical chemistry has garnered significant attention in the context mitigating global environmental contamination. In this study, we present two methodologies for environmentally friendly chromatography that enable simultaneous and specific determination Saxagliptin (SAX), metformin (MET), a pharmacopoeial impurity MET known as melamine (MEL). initial method employed is High-Performance Thin Layer Chromatography (HPTLC), which utilized 60 F 254 silica...

A cost-effective, selective, sensitive, and operational TLC-densitometric approach has been adapted for the concurrent assay of Hydroxyzine Hydrochloride (HYX), Ephedrine (EPH), Theophylline (THP) in their pure powder pharmaceutical forms. In innovative approach, HYX, EPH, THP were efficaciously separated quantified on a 60F254 silica gel stationary phase with chloroform-ammonium acetate buffer (9.5:0.5, v/v) adjusted to pH 6.5 using ammonia solution as mobile liquid system UV detection at...

Abstract Dapoxetine hydrochloride (DAP) and Tadalafil (TAD) were separated determined quantitatively using a validated green high-performance thin layer chromatographic (HPTLC) method in their binary mixtures either as raw materials or pharmaceutical formulations. The concentration ranges 0.1–1.6 0.2–2.5 μg/band for dapoxetine tadalafil, respectively, with accuracies of 98.93% ± 0.62 99.26% 1.39, respectively. Silica gel HPTLC F254 plates used to carry out the separation. mobile phase was...

Abstract Nowadays, when analysts develop a new method, they are taking into consideration the green aspects of developed method. Two chromatographic methods were for determination antidepressant pharmaceutical combination nortriptyline hydrochloride and fluphenazine in presence their potential impurities assessed using four tools; eco-scale, analytical greenness profile (AGP), metric approach (AGREE), procedure index (GAPI). Method (A) was RP-HPLC, which separation carried out on C 18 column...

ABSTRACT Accurate, selective, sensitive and precise HPTLC‐densitometric RP‐HPLC methods were developed validated for determination of bumadizone calcium semi‐hydrate in the presence its alkaline‐induced degradation product pharmaceutical formulation. Method A uses HPTLC‐densitometry, depending on separation quantitation TLC silica gel 60 F 254 plates, using hexane–ethyl acetate–glacial acetic acid (8:2:0.2, v/v/v) as a mobile phase followed by densitometric measurement bands at 240 nm. B...

A novel ecofriendly, cost and time saving high-performance thin-layer chromatographic method was developed validated for simultaneous determination of metoclopramide, ergotamine, caffeine, paracetamol in bulk pharmaceutical formulation. The separation carried out on silica gel plates, using ethyl acetate:ethanol:ammonia (9:1:0.1, v/v/v) as a developing system. Ultraviolet detection at 272 nm. resulting retention times were 0.15, 0.36, 0.49, 0.74 min paracetamol, respectively. greenness...

Recently, prednisolone has been used in treating many medical conditions, such as autoimmune diseases and cancer. It is also prescribed to mitigate the respiratory complications caused by COVID-19 infection. can cause some health complications, GIT ulcers, so it should be co-administered with proton-pump inhibitors, esomeprazole, prevent risk of ulcers. This work aims develop an ecofriendly sensitive TLC method for simultaneous determination esomeprazole their binary mixtures spiked human...

Abstract Two sensitive, straightforward and repeatable chromatographic techniques were developed for the determination of Cytarabine HCl Dexamethazone in their pure form spiked human plasma without prior separation. The drugs are used co-administered treatment Leukemia, a certain type blood cancer. Method (A) is an isocratic HPLC method; separation was accomplished on C18 column using eluting mixture 6.9 g/L Monobasic Sodium Phosphate pH 3: methanol (70:30, v/v) detection at 275 nm....

Abstract Two simple, sensitive, and reproducible methods were developed for the determination of alogliptin metformin hydrochloride in presence impurity “melamin” pure form pharmaceutical formulation. Method (A) was a thin layer chromatographic method which separation achieved using ethyl acetate–methanol–formic acid (6:3.8:0.2, by volume) as developing system followed densitometric scanning at 230 nm. (B) high‐performance liquid chromatography method; on C 18 column, mobile phase consisted...

Abstract Background: Dapoxetine (DAP) is a serotonin-norepinephrine reuptake inhibitor, and Tadalafil (TAD) phosphodiesterase type-5 inhibitor. Both are coformulated as tablets called Erectafil® for treatment of erectile ejaculation. Objective: DAP TAD were analyzed in their binary mixtures pharmaceutical formulations using two multivariate calibration chemometric models. Methods: Partial least-squares (PLS) linear support vector regression (SVR) models applied factor-four level experimental...

A validated RP-HPLC method was developed for the estimation of paracetamol, caffeine, metoclopramide and ergotamine simultaneously in bulk pharmaceutical formulation. The extended determination two paracetamol genotoxic nephrotoxic impurities degradation products namely p-aminophenol 4-chloroacetanilide. Separation done on octadecyl column (15 cm × 2.1 mm, 5 μm). Gradient elution performed using mobile phase consisting acetonitrile water (pH 3) ratio (20: 80, v/v) first 3 min, (50: 50, next...

Recently, the main interest of analytical chemistry researchers has been development green methods to minimize harmful effects on environment and natural life. Therefore, an RP-HPLC method was developed assessed regarding its greenness criteria using three assessment tools: eco-scale, metric approach a procedure index. This aims separate quantitatively determine co-administered drugs, namely pyridostigmine bromide (PYR), 6-mercaptopurine (MRC) prednisolone (PRD), in their tertiary mixture...

Sulfasalazine and pentoxifylline are co-prescribed together to treat psoriasis pemphigus vulgaris. is an anti-inflammatory, immunosuppressant, antibiotic drug, while a vasodilator immunosuppressant. The spectra of the two drugs plasma suffer from severe overlap.

Prednisolone (PRD) is an immunosuppressant and anti-inflammatory drug, although it may cause peptic ulcers as a side effect. Esomeprazole (ESO) used for the treatment of ulcers, therefore two drugs are co-administered in cases organ transplantation autoimmune diseases.This work aims to determine simultaneously, bulk, spiked human plasma by eliminating overlap their spectra interference matrix.Two simple effective updated chemometric models-principal component analysis (PCA) partial least...

Prednisolone, 6-mercaptopurine, and pyridostigmine bromide are co-administered together to treat a neuromuscular autoimmune disease called myasthenia gravis. Prednisolone 6-mercaptopurine immunosuppressant drugs. 6-Mercaptopurine is the active form of pro-drug azathioprine. Pyridostigmine cholinesterase enzyme inhibitor. Curently, green characteristics taken into account by analysts when they develop new methods.The spectra three compounds plasma highly overlapped, so this study aims remove...

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