A5048193232- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Protein purification and stability
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
BOKU University
2018-2024
Despite efforts to develop concepts for efficient antibody fragment (Fab) production in Escherichia coli (E. coli) and the high degree of similarity within this protein class, a generic platform technology is still not available. Indeed, feasible new Fab candidates remains challenging. In study, setup that enables direct characterization host cell response expression by utilizing genome-integrated (GI) systems established. Among multitude factors influence expression, variable domain,...
Abstract Background Escherichia coli is a cost-effective expression system for production of antibody fragments like Fabs. Various yield improvement strategies have been applied, however, Fabs remain challenging to produce. This study aimed characterize the gene response commonly used E. strains BL21(DE3) and HMS174(DE3) periplasmic Fab using RNA sequencing (RNA-seq). Two Fabs, Fabx FTN2, fused post-translational translocation signal sequence, were produced in carbon-limited fed-batch...
Antibody fragments such as Fab's require the formation of disulfide bonds to achieve a proper folding state. During their recombinant, periplasmic expression in Escherichia coli, oxidative is mediated by DsbA/DsbB system concert with ubiquinone. Thereby, overexpression linked respiratory chain, which not only immensely important for cell's energy household but also known major source reactive oxygen species. However, effects an increased demand and consequently required electron flux via...