A5014450728- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- Lipid Membrane Structure and Behavior
- Animal Virus Infections Studies
Rockefeller University
2022-2023
Heidelberg University
2023
University Hospital Heidelberg
2023
Abstract Feedback inhibition of humoral immunity by antibodies was first documented in 1909 1 . Subsequent studies showed that, depending on the context, can enhance or inhibit immune responses 2,3 However, little is known about how pre-existing influence development memory B cells. Here we examined cell response individuals who received two high-affinity anti-SARS-CoV-2 monoclonal and subsequently doses an mRNA vaccine 4–8 We found that recipients produced antigen-binding neutralizing...
Waves of SARS-CoV-2 infection have resulted from the emergence viral variants with neutralizing antibody resistance mutations. Simultaneously, repeated antigen exposure has generated affinity matured B cells, producing broadly receptor binding domain (RBD)-specific antibodies activity against emergent variants. To determine how might escape these antibodies, we subjected chimeric viruses encoding spike proteins ancestral, BA.1 or BA.2 to selection by 40 antibodies. We identify numerous...
Individuals who receive a third mRNA vaccine dose show enhanced protection against severe COVID-19, but little is known about the impact of breakthrough infections on memory responses. Here, we examine antibodies that develop after or fourth antigenic exposure by Delta Omicron BA.1 infection, respectively. A to antigen increases number B cells produce with comparable potency and breadth dose. infection increased variant-specific plasma antibody cell However, did not increase overall...
For successful infection of host cells and virion production, enveloped viruses, including Zika virus (ZIKV), extensively rely on cellular lipids. However, how protein-lipid interactions contribute to the viral life cycle remains unclear. Here, we employ a chemo-proteomics approach with bifunctional cholesterol probe show that is closely associated ZIKV structural protein prM. Bioinformatic analyses, reverse genetics alongside photoaffinity labeling assays, atomistic molecular dynamics...
Consecutive waves of SARS-CoV-2 infection have been driven in part by the repeated emergence variants with mutations that confer resistance to neutralizing antibodies Nevertheless, prolonged or antigen exposure generates diverse memory B-cells can produce affinity matured receptor binding domain (RBD)-specific likely contribute ongoing protection against severe disease. To determine how omicron might escape these broadly antibodies, we subjected chimeric viruses encoding spike proteins from...
Abstract Individuals that receive a 3 rd mRNA vaccine dose show enhanced protection against severe COVID19 but little is known about the impact of breakthrough infections on memory responses. Here, we examine antibodies develop after or 4 th antigenic exposure by Delta Omicron BA.1 infection, respectively. A to antigen increases number B cells produce with comparable potency and breadth dose. infection increased variant specific plasma antibody cell However, did not increase overall...