A5082075250- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV Research and Treatment
- DNA Repair Mechanisms
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- HIV/AIDS drug development and treatment
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
- Carcinogens and Genotoxicity Assessment
- Genomics and Chromatin Dynamics
- CAR-T cell therapy research
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Acute Lymphoblastic Leukemia research
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Long-Term Effects of COVID-19
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Viral-associated cancers and disorders
- HIV/AIDS Research and Interventions
Rockefeller University
2014-2024
Universidade de São Paulo
2014
Center of Molecular Immunology (Cuba)
2007-2014
Howard Hughes Medical Institute
1996-2012
Institute of Molecular Genetics
1990-1994
York University
1991
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected 78 million individuals and is responsible for over 1.7 deaths to date. Infection associated with the development of variable levels antibodies neutralizing activity, which can protect against infection in animal models1,2. Antibody decrease time, but, our knowledge, nature quality memory B cells that would be required produce upon reinfection not been examined. Here we report on humoral response a cohort 87 assessed at...
Abstract More than one year after its inception, the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) remains difficult to control despite availability of several working vaccines. Progress in controlling is slowed emergence variants that appear be more transmissible and resistant antibodies 1,2 . Here we report on a cohort 63 individuals who have recovered from COVID-19 assessed at 1.3, 6.2 12 months SARS-CoV-2 infection, 41% whom also...
DNA double-strand breaks (DSBs) represent a threat to the genome because they can lead loss of genetic information and chromosome rearrangements. The repair protein p53 binding 1 (53BP1) protects by limiting nucleolytic processing DSBs mechanism that requires its phosphorylation, but whether 53BP1 does so directly is not known. Here, we identify Rap1-interacting factor (Rif1) as an ATM (ataxia-telangiectasia mutated) phosphorylation-dependent interactor show absence Rif1 results in 5'-3'...
T follicular helper (T(FH)) cells are a specialized subset of effector that provide help to and thereby select high-affinity B in germinal centers (GCs). To examine the dynamic behavior T(FH) GCs mice, we used two-photon microscopy combination with photoactivatable fluorescent reporter. Unlike GC cells, which clonally restricted, distributed among all lymph nodes continually emigrated into follicle neighboring GCs. Moreover, newly activated invaded preexisting GCs, where they contributed...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection produces B cell responses that continue to evolve for at least a year. During time, memory cells express increasingly broad and potent antibodies are resistant mutations found in variants of concern 1 . As result, vaccination disease 2019 (COVID-19) convalescent individuals with currently available mRNA vaccines high levels plasma neutralizing activity against all tested 1,2 Here we examine evolution five months...
The Omicron variant of SARS-CoV-2 infected many vaccinated and convalescent individuals
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has infected 78 million individuals and is responsible for over 1.7 deaths to date. Infection associated with development of variable levels antibodies neutralizing activity that can protect against infection in animal models. Antibody decrease time, but the nature quality memory B cells would be called upon produce re-infection not been examined. Here we report on humoral response a cohort 87 assessed at 1.3 6.2 months after...
Significance A reservoir of latently infected cells poses the greatest challenge to HIV-1 eradication. Efforts develop strategies eliminate have been hampered, in part, by lack a precise understanding cellular and molecular nature this reservoir. We describe new method analyze replication-competent latent quantitatively qualitatively. find that over 50% viruses form part groups with identical env sequences. However, negative correlation exists between integrated proviral clones viruses, such...
Class switch recombination (CSR) in B lymphocytes is initiated by introduction of multiple DNA double-strand breaks (DSBs) into (S) regions that flank immunoglobulin heavy chain ( IgH ) constant region exons. CSR completed joining a DSB the donor Sμ to downstream acceptor S (e.g., Sγ1) end-joining. In normal cells, many junctions are mediated classical nonhomologous end-joining (C-NHEJ), which employs Ku70/80 complex for recognition and XRCC4/DNA ligase 4 ligation. Alternative (A-EJ)...
The classical nonhomologous end-joining (C-NHEJ) DNA double-strand break (DSB) repair pathway employs the Ku70/80 complex (Ku) for DSB recognition and XRCC4/DNA ligase 4 (Lig4) ligation. During IgH class switch recombination (CSR) in B lymphocytes, (S) region DSBs are joined by C-NHEJ to form junctions either with short microhomologies (MHs; "MH-mediated" joins) or no homologies ("direct" joins). In absence of XRCC4 Lig4, substantial CSR occurs via "alternative" (A-EJ) that generates largely...
Programmed genetic rearrangements in lymphocytes require transcription at antigen receptor genes to promote accessibility for initiating double-strand break (DSB) formation critical DNA recombination and repair. Here, we showed that activated B cells deficient the PTIP component of MLL3 (mixed-lineage leukemia 3)-MLL4 complex display impaired trimethylation histone 3 lysine 4 (H3K4me3) initiation downstream switch regions immunoglobulin heavy-chain (Igh) locus, leading defective class...