A5072461364- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Eosinophilic Esophagitis
- SARS-CoV-2 detection and testing
- Transgenic Plants and Applications
- Long-Term Effects of COVID-19
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and Retrovirus Studies
- Viral gastroenteritis research and epidemiology
- Lymphoma Diagnosis and Treatment
Rockefeller University
2017-2022
Centre d’Immunologie de Marseille-Luminy
2014-2017
Inserm
2014-2017
Aix-Marseille Université
2014-2017
Centre National de la Recherche Scientifique
2014-2017
During the coronavirus disease-2019 (COVID-19) pandemic, severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has led to infection of millions people and claimed hundreds thousands lives. The entry virus into cells depends on receptor-binding domain (RBD) spike (S) protein SARS-CoV-2. Although there is currently no vaccine, it likely that antibodies will be essential for protection. However, little known about human antibody response SARS-CoV-21–5. Here we report 149...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected 78 million individuals and is responsible for over 1.7 deaths to date. Infection associated with the development of variable levels antibodies neutralizing activity, which can protect against infection in animal models1,2. Antibody decrease time, but, our knowledge, nature quality memory B cells that would be required produce upon reinfection not been examined. Here we report on humoral response a cohort 87 assessed at...
Abstract More than one year after its inception, the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) remains difficult to control despite availability of several working vaccines. Progress in controlling is slowed emergence variants that appear be more transmissible and resistant antibodies 1,2 . Here we report on a cohort 63 individuals who have recovered from COVID-19 assessed at 1.3, 6.2 12 months SARS-CoV-2 infection, 41% whom also...
Human dimeric IgA antibodies are potent neutralizers of SARS-CoV-2.
Light- and dark-zone death dynamics Germinal centers (GCs) are areas within lymphoid organs where mature B cells expand differentiate during normal immune responses. GCs separated into two anatomic compartments: the dark zone, divide undergo somatic hypermutation, light they selected for affinity-enhancing mutations after interacting with T follicular helper cells. Mayer et al. studied apoptosis reporter mice found that both GC zones experience very high rates of (see Perspective by Bryant...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has infected 78 million individuals and is responsible for over 1.7 deaths to date. Infection associated with development of variable levels antibodies neutralizing activity that can protect against infection in animal models. Antibody decrease time, but the nature quality memory B cells would be called upon produce re-infection not been examined. Here we report on humoral response a cohort 87 assessed at 1.3 6.2 months after...
Abstract During the COVID-19 pandemic, SARS-CoV-2 infected millions of people and claimed hundreds thousands lives. Virus entry into cells depends on receptor binding domain (RBD) spike protein (S). Although there is no vaccine, it likely that antibodies will be essential for protection. However, little known about human antibody response to 1–5 . Here we report 149 convalescent individuals. Plasmas collected an average 39 days after onset symptoms had variable half-maximal neutralizing...
Group 3 innate lymphoid cells (ILC3s) are composed of subsets that either positive or negative for the natural cytotoxicity receptor (NCR) NKp46 (encoded by Ncr1). ILC3s located at mucosal sites, such as in intestine and lung, where they exposed to billions commensal microbes potentially harmful pathogens. Together with T cells, various ILC3 maintain balance between homeostasis immune activation. Through genetic mapping, we identified a previously uncharacterized subset NCR(-) mice...
Memory B cells comprise a heterogenous group of that differ in origin and phenotype. During the early phases immune response, activated can differentiate into IgM-expressing memory cells, short-lived plasma or seed germinal centers (GCs). The compartment is subsequently enriched by have been through several rounds division selection GC. Here, we report on use an unbiased lineage-tracking approach to explore origins properties cell subsets mice with intact system. We find continue throughout...
Antibody responses are characterized by increasing affinity and diversity over time. Affinity maturation occurs in germinal centers a mechanism that involves repeated cycles of somatic mutation selection. How antibody diversify while also undergoing is not as well understood. Here, we examined center (GC) dynamics tracking B cell entry, division, mutation, specificity. Our experiments show naive cells continuously enter GCs where they compete for T help undergo clonal expansion. Consistent...
Natural killer (NK) cells are innate lymphoid with antitumor functions. Using an N-ethyl-N-nitrosourea (ENU)–induced mutagenesis screen in mice, we identified a strain NK cell deficiency caused by hypomorphic mutation the Bcl2 (B lymphoma 2) gene. Analysis of these mice and conditional deletion revealed nonredundant intrinsic requirement for BCL2 survival. In cycle were protected against apoptosis, counts restored inflammatory conditions, suggesting redundant role proliferating cells....
Over one year after its inception, the coronavirus disease-2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains difficult to control despite availability of several excellent vaccines. Progress in controlling is slowed emergence variants that appear be more transmissible and resistant antibodies 1,2 . Here we report on a cohort 63 COVID-19-convalescent individuals assessed at 1.3, 6.2 12 months infection, 41% whom also received mRNA vaccines...
Abstract SARS-CoV-2 primarily infects cells at mucosal surfaces. Serum neutralizing antibody responses are variable and generally low in individuals that suffer mild forms of the illness. Although potent IgG antibodies can neutralize virus, less is known about secretory such as IgA might impact initial viral spread transmissibility from mucosa. Here we characterize response to a cohort 149 individuals. plasma correlate with clones IgM, producing B derived common progenitors evident. Plasma...
The analysis of B cell receptors (BCR) from single cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization antibody genes that encode BCRs. We used this method analyze BCRs different mouse populations somatic hypermutations, clonal phylogenic relationships, their affinity cognate antigen. For complete details on use execution protocol, please refer Viant et al. (2020).