A5101835095- Epigenetics and DNA Methylation
- Fungal and yeast genetics research
- Redox biology and oxidative stress
- Glutathione Transferases and Polymorphisms
- Cancer-related gene regulation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Growth Hormone and Insulin-like Growth Factors
- Genomics, phytochemicals, and oxidative stress
- Enzyme Structure and Function
- Retinal Diseases and Treatments
- Selenium in Biological Systems
- Nitrogen and Sulfur Effects on Brassica
- Vascular Malformations Diagnosis and Treatment
- Trace Elements in Health
- Protein Tyrosine Phosphatases
- Thyroid Disorders and Treatments
- Retinal and Macular Surgery
- Estrogen and related hormone effects
- Retinal Development and Disorders
- Natural product bioactivities and synthesis
- Protein Kinase Regulation and GTPase Signaling
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Ginseng Biological Effects and Applications
National Institutes of Health
2011-2025
National Institute of Diabetes and Digestive and Kidney Diseases
2007-2023
Korea Basic Science Institute
2012-2015
Ulsan College
2010-2014
University of Ulsan
2010-2014
University of California, San Diego
2013
Asan Medical Center
2010
Center for Cancer Research
2004-2005
National Cancer Institute
2004-2005
University of Illinois Urbana-Champaign
2005
Covalent modifications of histones, such as acetylation and methylation, play important roles in the regulation gene expression. Histone lysine methylation has been implicated both activation repression, depending on specific (K) residue that becomes methylated state (mono-, di-, or trimethylation). Methylation K4, K9, K36 histone H3 shown to be reversible can removed by site-specific demethylases. However, enzymes antagonize K27 (H3K27), an epigenetic mark for embryonic stem cell...
PTIP, a protein with tandem BRCT domains, has been implicated in DNA damage response. However, its normal cellular functions remain unclear. Here we show that while ectopically expressed PTIP is capable of interacting response proteins including 53BP1, endogenous and novel PA1 are both components Set1-like histone methyltransferase (HMT) complex also contains ASH2L, RBBP5, WDR5, hDPY-30, NCOA6, SET domain-containing HMTs MLL3 MLL4, substoichiometric amount JmjC putative demethylase UTX....
Enhancers play a central role in cell-type-specific gene expression and are marked by H3K4me1/2. Active enhancers further H3K27ac. However, the methyltransferases responsible for H3K4me1/2 on remain elusive. Furthermore, how these enzymes function to regulate is unclear. In this study, we identify MLL4 (KMT2D) as major mammalian H3K4 mono- di-methyltransferase with partial functional redundancy MLL3 (KMT2C). Using adipogenesis myogenesis model systems, show that exhibits cell-type-...
To investigate the role of histone H3K27 demethylase UTX in embryonic stem (ES) cell differentiation, we have generated knockout (KO) and enzyme-dead knock-in male ES cells. Deletion X-chromosome-encoded gene cells markedly decreases expression paralogous UTY encoded by Y chromosome, but has no effect on global H3K27me3 level, Hox expression, or self-renewal. However, KO show severe defects mesoderm differentiation induction Brachyury, a transcription factor essential for development....
Programmed genetic rearrangements in lymphocytes require transcription at antigen receptor genes to promote accessibility for initiating double-strand break (DSB) formation critical DNA recombination and repair. Here, we showed that activated B cells deficient the PTIP component of MLL3 (mixed-lineage leukemia 3)-MLL4 complex display impaired trimethylation histone 3 lysine 4 (H3K4me3) initiation downstream switch regions immunoglobulin heavy-chain (Igh) locus, leading defective class...
In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, part, by Wee1 kinase. Timely budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations the septin collar at bud neck lead to stabilization, delaying G 2 /M transition. recruited and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 (Nim1 ortholog), a negative regulator Wee1, required for efficient localization seems not phosphorylate Swe1. Here, we show...
Myeloid/lymphoid or mixed-lineage leukemia ( MLL )-family genes encode histone lysine methyltransferases that play important roles in epigenetic regulation of gene transcription. are frequently mutated human cancers. Unlike MLL1, MLL2 (also known as ALR/MLL4) and its homolog MLL3 not well-understood. Specifically, little is regarding the extent global involvement expression mechanism underlying alterations driving tumorigenesis. Here we profile loci targeted by MLL2. A combinatorial analysis...
Mediator is a general coactivator complex connecting transcription activators and RNA polymerase II. Recent work has shown that the nuclear receptor-interacting MED1/TRAP220 subunit of required for peroxisome proliferator-activated receptor gamma (PPARgamma)-stimulated adipogenesis mouse embryonic fibroblasts (MEFs). However, molecular mechanisms remain undefined. Here, we show an intracellular PPARgamma-Mediator interaction requires two LXXLL recognition motifs on and, furthermore, intact...
Objectives The purpose of this study was to evaluate the usefulness retroauricular hair line incision (RAHI) in partial superficial parotidectomy by comparison with modified Blair or facelift incision. Methods Medical records 73 patients benign parotid tumor who underwent were retrospectively reviewed. Size and location tumors, operative time, occurrence facial nerve paralysis Frey's syndrome, cosmetic outcomes compared among RAHI, (FLI), (MBI) groups. Results RAHI group showed better...
Myxomatous mitral valve disease (MMVD) in dogs can be treated with transcatheter edge-to-edge repair (TEER), an emerging minimally invasive surgical technique. However, reports on prognostic markers for preoperative evaluation remain limited. This study evaluated the association of N-terminal pro-brain natriuretic peptide (NT-proBNP) and cardiac troponin I (cTnI) levels procedure safety undergoing TEER. A retrospective analysis was conducted 25 diagnosed stage C (n = 18) or D 7) MMVD that...
Abstract In mammalian cells, distinct H3K4 methylation states are created by deposition of methyl groups multiple complexes histone lysine methyltransferase 2 (KMT2) family proteins. For comprehensive analyses that directly compare the catalytic properties all six human KMT2 complexes, we employed a biochemically defined system reconstituted with recombinant core (KMT2CoreCs) containing minimal components required for nucleosomal activity. We found each KMT2CoreC generates and different...
Thyroid hormones (THs) are powerful regulators of metabolism with major effects on body weight, cholesterol, and liver fat that have been exploited pharmacologically for many years. Activation gene expression by TH action is canonically ascribed to a hormone-dependent “switch” from corepressor activator binding thyroid hormone receptors (TRs), while the mechanism TH-dependent repression controversial. To address this, we generated mouse line in which endogenous TRβ1 was epitope-tagged allow...
Cone photoreceptor diversity allows detection of wavelength information in light, the first step color (chromatic) vision. In most mammals, cones express opsin photopigments for sensitivity to medium/long (M, "green") or short (S, "blue") wavelengths and are differentially arrayed over retina. Cones appear early retinal neurogenesis but little is understood subsequent control these postmitotic neurons, because cone populations sparse and, apart from opsins, poorly defined. It also a...
Peroxisome proliferator-activated receptor-binding protein (PBP), also known as thyroid hormone receptor-associated 220/vitamin D receptor-interacting 205/mediator 1, an anchor for multisubunit mediator transcription complex, functions a coactivator nuclear receptors. Disruption of the PBP gene results in embryonic lethality around day 11.5 by affecting placental and multiorgan development. Here, we report that targeted deletion liver parenchymal cells (PBP Liv-/- ) abrogation hypertrophic...
Class switch recombination (CSR) diversifies antibodies for productive immune responses while maintaining stability of the B-cell genome. Transcription at immunoglobulin heavy chain ( Igh ) locus targets CSR-associated DNA damage and is promoted by BRCT domain-containing PTIP (Pax transactivation domain-interacting protein). Although a unique component mixed-lineage leukemia 3 (MLL3)/MLL4 chromatin-modifying complex, mechanisms how promotes transcription remain unclear. Here we dissected...
This work aimed to determine some pharmacological properties of non-fermented (WG) and fermented (FWG) extracts cultured wild ginseng root.WG was treated with Bifidobacterium longum generate FWG. Ginsenoside patterns were analysed using thin-layer chromatography high-performance liquid chromatography. The effect WG FWG on reactive oxygen species (ROS) examined in lipopolysaccharide-stimulated RAW264.7 macrophage cells. Intracellular ROS detected by flow cytometry. Nitrite culture supernatant...
An effective way of inducing both mucosal and systemic immune responses to protect against Actinobacillus pleuropneumoniae serotype 2 Korean isolate was examined in mice by oral immunization using Saccharomyces cerevisiae expressing the ApxIIA protein. The immunogenicity yeast-derived antigen confirmed challenge test ApxIIA-specific IgG antibody response assay. group subcutaneously immunized with protein extracted from yeast showed a higher survival rate after challenging A. level serum than...
To assess palatine tonsil size relative to degree of obesity in children with sleep-disordered breathing (SDB).Case-control study.Tertiary care rhinology clinic.We compared the subjective and objective size, including height (TH), width (TW), thickness (TT), weight (TWt), volume (TV), 26 obese age- gender-matched control SDB, 29 overweight matched controls SDB.Despite similar children, TH, TW, TT, TWt, was larger than mean TV significantly greater SDB (8.53 +/- 3.30 mL vs 6.90 2.05 mL; 95%...
Abstract Significant loss of body weight (SLW) by patients treated for squamous cell carcinomas the oral cavity and oropharynx (OSCC) may affect treatment completion results. We assessed factors predicting SLW its correlation with disease-free survival (DFS) in these patients. evaluated 226 consecutive previously untreated, operable OSCC whose was recorded before, during, up to 1 year after treatment. defined as ≥10% reduction pretreatment weight. Clinicopathologic parameters were compared...
Background: Human PAX‐Interacting Protein 1 (PAXIP1)‐associated glutamate rich protein (PAGR1, also known as PA1) originally was discovered part of a complex containing PAXIP1 and histone H3K4 methyltransferases MLL3 MLL4, suggesting role in epigenetic gene regulation. Further vitro studies suggested additional functions DNA damage repair transcription. However, vivo analysis PAGR1 function has been lacking. Results: Here we show that expression the cognate mouse Pagr1a is found...
Transcription factors C/EBPβ and C/EBPδ are induced within hours after initiation of adipogenesis in culture. They directly promote the expression master adipogenic transcription peroxisome proliferator-activated receptor γ (PPARγ) C/EBPα required for vivo. However, mechanism that controls induction remains elusive. We previously showed histone methyltransferases MLL3/MLL4 associated PTIP PPARγ during adipogenesis. Here, we show MLL3/MLL4/PTIP-associated protein PAGR1 (also known as PA1)...