Multidrug resistance (MDR) is a major cause of failure cancer chemotherapy. Fifty-eight ecdysteroids, herbal analogues the insect molting hormone and their semisynthetic derivatives, were tested for activity against L5178 mouse T-cell lymphoma cells (non-MDR) subcell line transfected with pHa MDR1/A retrovirus overexpressing human ABCB1 efflux pump (MDR cell line). The compounds showed very low antiproliferative activities but modulated rhodamine 123 mediated by transporter. Roughly...

Mycochemical study of the mushroom Gymnopus fusipes led to discovery two new cyclopeptides. The compounds, named as gymnopeptides A and B, are unprecedented highly N-methylated cyclic octadecapeptides. Detailed spectroscopic studies, Marfey's analysis, a preliminary molecular modeling suggested that both natural β hairpins. isolated compounds exhibited striking antiproliferative activity on several human cancer cell lines, with nanomolar IC50 values.

Bioassay-guided fractionation of the chloroform extract Tapinella atrotomentosa led to isolation four secondary metabolites 1⁻4. Two compounds are lactones—osmundalactone (1) and 5-hydroxy-hex-2-en-4-olide (2)—while 3 4 were identified as terphenyl quinones, spiromentins C B, respectively. The structures established on basis NMR MS spectroscopic analysis. isolated fungal evaluated for their antibacterial activities against several Gram-positive negative bacteria. In addition, synergistic...

An in-depth chemical study of the fungus Gymnopus fusipes led to discovery two new cyclooctadecapeptides, gymnopeptides C and D, besides known A B. Spectroscopic studies, as well Marfey's analysis, molecular modeling studies revealed characteristic structural features these cyclopeptides. Gymnopeptides A–D demonstrated antiproliferative activity on A375 human cancer cell line, induced apoptosis by upregulation caspase-3 activity, acted a suppressor migration.

This study aimed to screen the antibacterial activity of 160 extracts 40 mushroom species, collected in Hungary, against 11 standard bacterial strains and 9 clinical isolates. The further objective this work was evaluate capacity active fungal potentiate action antibiotics resistant pathogens. Disc-diffusion method applied for screening extracts. Microdilution used determine minimum inhibitory concentrations. were different micro-organisms (multiresistant Acinetobacter baumannii Pseudomonas...

Investigation of the ecdysteroid constituents herb Ajuga reptans var. resulted in isolation three new ecdysteroids, named reptanslactone A (2), B (3), and sendreisterone (5), known 24-dehydroprecyasterone (1) breviflorasterone (4). The structures compounds 1−5 were determined by spectroscopic methods including one- two-dimensional NMR measurements.

Twelve compounds (1⁻12) were isolated from the methanol extract of brick cap mushroom (Hypholoma lateritium (Schaeff.) P. Kumm.). The structures elucidated using extensive spectroscopic analyses, including NMR and MS measurements. Lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol (1) 8-hydroxy-13-oxo-9E,11E-octa-decadienoic acid (2) identified as new natural products, together with ten known compounds, which 3β-hydroxyergosta-7,22-diene (4), demethylincisterol A2 (5), cerevisterol...

The detailed chemical analysis of the methanol extract Meripilus giganteus (Pers.) P. Karst. led to isolation two new cerebrosides, mericeramides A (1) and B (2) together with cerebroside (3), ergosterol (4), 3β-hydroxyergosta-7,22-diene (5), cerevisterol (6), 3β-hydroxyergosta-6,8(14),22-triene (7), 3β-O-glucopyranosyl-5,8-epidioxyergosta-6,22-diene (8) (11E,13E)-9,10-dihydroxy-11,13-octadecadienoic acid (9). structures compounds were determined on basis NMR MS spectroscopic analysis....

Investigation of the methanol extract poroid fungus Fuscoporia torulosa resulted in isolation a novel triterpene, fuscoporic acid (1), together with inoscavin A and its previously undescribed Z isomer (2 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic (6), natalic (7), ergosta-7,22-diene-3-one (8). The structures fungal compounds were determined on basis NMR MS spectroscopic analyses, as well molecular modeling studies. Compounds 1, 6–8 examined for their antibacterial...

Mushrooms represent a remarkable and yet largely unexplored source of new, biologically active natural products. In this work, we report on the xanthine oxidase (XO) inhibitory activity 47 wild-growing mushrooms native to Hungary. Aqueous organic (n-hexane, chloroform, 50% methanol) extracts selected from different families were screened for their XO activities. Among 188 investigated, chloroform methanol fractions proved be most effective. Some species exhibited high activity, e.g.,...

Inonotus nidus-pici is a sterile conk which produces macrofungus, neglected Central-Eastern European relative of the prized obliquus, also known as chaga. Investigation methanol extract poroid fungus I. resulted in isolation citropremide (1), 3,4-dihydroxybenzalacetone (2) , lanosterol (3), ergost-6,8,22-trien-3β-ol (4), and ergosterol peroxide (5). The structures fungal compounds were determined on basis one- two-dimensional NMR MS spectroscopic analysis. Compounds 1–2 4–5 evaluated for...

Two new and one known ecdysteroids were identified in the methanolic extract of roots Serratula wolffii. The compounds isolated ponasterone A-22-apioside (1) 3-epi-shidasterone (3), together with 3-epi-22-deoxy-20-hydroxyecdysone (2). structures 1-3 determined by extensive spectroscopic techniques, including one- two-dimensional NMR methods.

Ten representative Central European phellinoid Hymenochaetaceae species (Phellinus sensu lato) were selected to examine their potential pharmacological activity. In this study 40 organic (n-hexane, chloroform, 50% methanol) and aqueous extracts with different polarities analyzed for antimicrobial, antioxidant, xanthine oxidase (XO)−inhibitory properties. Fomitiporia robusta, Fuscoporia torulosa, Phellopilus nigrolimitatus, Porodaedalea chrysoloma showed moderate antibacterial activity;...

Abstract Hypholoma lateritium is an edible macrofungus with a common distribution in Europe, North America, and the Far East. The aim of this study was to investigate potential anti‐inflammatory effects H. extracts its isolated steroids: fasciculic acid B, fasciculol E, C, lanosta‐7,9(11)‐diene‐12 β ,21 α ‐epoxy‐2 ,3 ,24 ,25‐tetraol, F, demethylincisterol A2. Organic (hexane, chloroform 50 % methanol) water were subjected vitro assays determine pro‐inflammatory protein levels, such as...

The plant kingdom has remained an inexhaustible reservoir of compounds with a potential further therapeutic use. Ecdysteroids are such biologically active which were discovered in insects, and later their presence was confirmed many species. They have tremendous the most modern therapy (gene-switch systems). occur plants together several other types natural similar chromatographic behavior. On hand, usually contain series ecdysteroids related chemical structures. Sophisticated analytical...

In the present work, aqueous and organic extracts of 16 Basidiomycetes mushrooms 1 Ascomycetes mushroom were investigated in vitro for their antiproliferative activity against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma), A2780 (ovarian MCF7 (breast adenocarcinoma) cells, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. A total 68 n-hexane, chloroform, 50% methanol, water selected species screened potential cell growth inhibitory...

The chemical analysis of the methanol extract Porodaedalea chrysoloma (Fr.) Fiasson & Niemela afforded isolation five compounds (1-5). first two are phenolic derivatives: methyl (E)-3-(4-methoxycar-bonylphenoxy)-acrylate (1) is a new natural product, while 3-(4-methoxycarbonylphenoxy)-propionate (2) was isolated from source for time. triterpene steroids ergone (3), 3β-hydroxyergosta-7,22-diene (4), and ergosterol (5) have not been previously identified in this species. structures were...

Investigation of the chloroform and ethyl acetate extracts edible mushroom Pholiota populnea led to isolation eight triterpenes, undescribed natural products pholiols E-K known (+)-clavaric acid. HRESIMS 1D 2D NMR spectroscopy were employed determine structures compounds. The NOESY spectra used assign relative configurations triterpenes. isolated compounds screened for their anti-inflammatory activity on cyclooxygenase (COX-1 COX-2), lipoxygenase (5-LOX 15-LOX) inhibitory assays....

Pholiols L-S (1−8), eight undescribed triterpenes were isolated from the sporocarps of mushroom Pholiota populnea. Various chromatographic techniques, such as open column chromatography, flash gel filtration, preparative thin layer and HPLC, applied to purify compounds. The structure elucidation was carried out by spectroscopic analysis, including 1D (1H NMR 13C JMOD) 2D (1H-1H COSY, HSQC, HMBC NOESY) HRESIMS experiments. compounds had lanostane (1−7) or trinorlanostane (8) skeletons; all...

Xylobolus subpileatus is a widely distributed crust fungus reported from all continents except Antarctica, although considered rare species in several European countries. Profound mycochemical analysis of the methanol extract X. resulted isolation seven compounds (1–7). Among them, (3β,22E)-3-methoxy-ergosta-4,6,814,22-tetraene (1) new natural product, while NMR assignment its already known epimer (2) has been revised. In addition to benzohydrofuran derivative fomannoxin (3), four...