Abstract Cold atmospheric helium plasma jets were fabricated and utilized for plasma–cell interactions. The effect of operating parameters jet design on the generation specific reactive oxygen nitrogen species (RONS) within cells cellular response investigated. It was found that treatment induced overproduction RONS in various cancer cell lines selectively. under a relatively low applied voltage detachment cells, reduction viability apoptosis, while higher led to necrosis our case. To...

By using an atmospheric pressure plasma jet driven by pulsed dc voltage with repetition rate of several tens kilohertz, we were able to induce apoptosis in cultured human breast cancer cells (MCF-7). The apoptotic changes treatment detected flow cytometry and fluorescence staining assay. A significant portion these was observed exhibit the fragmentation. Helium additive O2 gas found be effective induction apoptosis. This provides mode cell therapy.

The effects of atmospheric pressure plasma jet on cancer cells (human lung carcinoma cells) and normal (embryonic kidney bronchial epithelial were investigated. Using a detection dye, the production intracellular reactive oxygen species (ROS) was found to be increased in plasma-treated compared non-treated gas flow-treated cells. A significant overproduction ROS reduction cell viability induced by exposure Normal observed less affected plasma-mediated ROS, changed. selective effect provides...

The atmospheric pressure helium plasma jet driven by pulsed dc voltage was utilized to treat human lung cancer cells in vitro. properties of plume were adjusted the injection type and flow rate additive oxygen gas jet. characteristics such as length, electric current optical emission spectra (OES) measured at different rates helium. length total decreased with an increase rate. electron excitation temperature estimated Boltzmann plot from several excited lines increased slightly flow. atom...

Helium/oxygen atmospheric pressure plasma jets driven by pulsed dc voltage with repetition rate of several tens kilohertz are utilized for plasma-cell interactions. The effect operating parameters on the apoptosis cultured human bladder (EJ) cancer cells is investigated. such as applied voltage, pulse frequency, and duty ratio determine dose. apoptotic changes in treatment detected staining assay flow cytometry. Apoptosis rates observed to correlate well both dose levels intracellular...

Bacterial inactivation experiment was performed using atmospheric pressure microplasma jets driven by radio-frequency wave of 13.56 MHz and low frequency several kilohertz. With addition a ground ring electrode, the discharge current, optical emission intensities from reactive radicals, sterilization efficiency were enhanced significantly. When oxygen gas added to helium at flow rate 5 SCCM, enhanced. From survival curve Escherichia coli, primary role in played species with minor aid heat,...

A microplasma jet with a capillary electrode working at atmospheric pressure is developed to create nonthermal plasma. This can be operated an excitation frequency either in several tens of kilohertz ac range (or pulsed voltage repetition rate range) or radio-frequency range. The gas, helium argon, and the additive oxygen, are fed into plasma jet. discharge has been characterized by optical emission spectroscopy. electrical property studied means current probes. dynamic nature plume...

An atmospheric pressure plasma jet array source driven by a pulsed bipolar voltage of several tens kilohertz was characterized and utilized for cancer cell treatment. Electrical optical emission characteristics were obtained as functions the applied voltage, gas flow rate, pulse frequency, optimal operating parameters obtained. The intensities from reactive oxygen nitrogen species (RONS) in phase, such OH, NO, H, O, measured spectroscopy. In plasma-liquid interaction experiment, OH...

An atmospheric pressure plasma jet source driven by pulsed wave of several tens kilohertz and sinusoidal was designed characterized. A newly consists a sharpened tungsten pin electrode covered with cone type Teflon layer confined in Pyrex tube. This structure provides an efficient ignition since the electric field is concentrated on end electrode. Using electrical optical characterization, properties bullet were explored. For Ar at low duty cycles, volume, speed, luminosity became larger,...

Atmospheric pressure plasma jets employing nitrogen, helium, or argon gases driven by low-frequency (several tens of kilohertz) ac voltage and pulsed dc were fabricated characterized. The changes in discharge current, optical emission intensities from reactive radicals, gas temperature, plume length with the control parameters measured compared. include applied voltage, working gas, flow rate. As an application to plasma-cancer cell interactions, effects atmospheric jet on morphology...

Epithelial‐mesenchymal transition ( EMT ) is a critical response during cancer cell metastasis. In this study, we provide evidence that uncoordinated 51‐like kinase 2 ULK 2) regulates . Induction of autophagy by inhibition mammalian target rapamycin complex 1 mTORC 1) or disruption silencing raptor significantly enhanced , however, rictor had no effect. Knockdown expression induced autophagy, and migration but suppressed proliferation as well tumor growth in xenotransplantation model,...

In this work, an atmospheric pressure plasma jet was fabricated and studied for plasma–liquid interactions. The consists of a quartz-covered pin electrode outer quartz tube with tapered nozzle. Using the current–voltage (I-V) optical emission characteristics jet, density speed plume were investigated. spectra clearly indicated excited NO, O, OH, N2, N2+ in plumes. Then jets applied to deionized water. We investigated effects operating parameters such as voltage, pulse frequency, gas flow...

An atmospheric-pressure plasma jet array source driven by a pulsed bipolar wave of several tens kilohertz was designed and characterized. This assembled with each individual module. Thus, it is possible to adjust the area treatment arranging number jets. It has potential greatly enhance scale surface over that single jet. Multiple plumes were generated propagated grounded-surface relatively uniformly without an auxiliary circuit. Due large separation between jet, bullets propagate through...

Abstract Atmospheric pressure microplasma jet sources driven by radio‐frequency wave of 13.56 MHz and low frequency continuous several kHz ac were fabricated characterized. The source consists an ac‐driven copper wire (needle) surrounded dielectric layer that are placed in a glass tube, ground plane electrode (pin‐to‐plane configuration). basic physical chemical properties the plasma sources, such as optical emission spectrum, gas temperature, power deposition investigated. With various...

This paper tests various design and operation parameters in atmospheric-pressure helium plasma jets generated with a pin electrode to provide environment well suited for material processing or biomedical applications. The effects of the materials (Cu, W, Al), position, width ground ring on characteristics are studied. inner quartz tube encompassing two types outer tubes (straight tapered cylinder shape) employed, their investigated. electrical measured by current-voltage measurements...

Atmospheric pressure pulsed helium plasma jets are utilized for plasma-cell interactions. The effect of operating parameters such as applied voltage, pulse repetition frequency, and duty ratio on the generation specific reactive oxygen nitrogen species in gas liquid phases within cells is investigated. apoptotic changes detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling assay caused exposure observed to correlate well with levels extracellular...

This paper explores the effects of tube shape and pin electrode diameter on plume generation electrical characteristics in atmospheric pressure helium plasma jets generated from a centered-pin inside dielectric tube. The quartz-covered (or bare) different shapes (straight cylinder or conical shape) are employed their investigated. Optical emission spectra obtained at positions along coaxial direction to determine axial distributions electron excitation temperature for straight jets....

Plasma-liquid and plasma-cell interactions were investigated using an atmospheric pressure dc microsecond-pulsed helium plasma jet. We the effects of electrical parameters such as applied voltage pulse width (determined by frequency duty ratio) on production reactive species in gas/liquid phases DNA damage responses cancer cells. The densities including OH radicals estimated inside plasma-treated liquids a chemical probe method, nitrite concentration was detected Griess assay. Importantly,...

Effects of control parameters (applied voltage, duty cycle, and gas flow rate) on the propagation plasma bullets are investigated in a pulsed atmospheric pressure argon jet. Images voltage rising falling phases presented. The applied pulse duration, rate observed to influence bullet characteristics.

Regulation of vascular smooth muscle cell (VSMC) phenotype plays an essential role in many cardiovascular diseases. In the present study, we provide evidence that krüppel-like factor 8 (KLF8) is for tumor necrosis α (TNFα)-induced phenotypic conversion VSMC obtained from thoracic aorta 4-week-old SD rats. Stimulation contractile VSMCs with TNFα significantly reduced marker gene expression and KLF8. The KLF8 was blocked by stimulation ERK-dependent manner. promoter region contained putative...

Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 human umbilical vein cells (HUVECs) significantly delayed proliferation, migration capillary-like tube formation. In addition, mice lacking showed impaired angiogenesis with reduced activation, abnormal vessel formation hemorrhage. Finally, tumor was irregular size shape blood vessels. These...

Atmospheric pressure helium plasma jets driven by a low-frequency bipolar microsecond-pulsed voltage were characterized. Some characteristic features of discussed with an emphasis on the temporal evolution discharge current and light emission. The electrical optical characteristics jet exhibited quite strong dependence pulse width oxygen gas admixture. We investigated effects admixture production reactive species in liquid phases. concentrations measured at plasma-liquid surface inside...