Abstract Dendritic cells (DC) are APCs that able to stimulate or inhibit immune responses, depending on levels of expression MHC class I and II costimulatory molecules cytokines. Our previous studies have suggested the observed contralateral effect, where injection a vector carrying certain immunomodulatory genes into one joint resulted in inhibition arthritis untreated joints, is mediated by vivo modification DC. Therefore, we examined ability genetically modified DC suppress established...

We previously have demonstrated the ability of primary murine bone marrow-derived DC (BM-DC), genetically modified by adenoviral infection to express FasL, inhibit progression established collagen-induced arthritis (CIA) following systemic delivery. Here we demonstrate that exosomes derived from BM-DC expressing FasL are able inflammation in a footpad model delayed-type hypersensitivity (DTH). Local administration (Exo/FasL) as well parental DC/FasL resulted significant reduction swelling...

Abstract In this study, we demonstrate that genetically modified bone marrow-derived dendritic cells (DC) and exosomes derived from the DC, expressing either secreted IL-4 or membrane-bound IL-4, can reduce severity incidence of established collagen-induced arthritis inhibit inflammation delayed-type hypersensitivity (DTH) in mice. The ability DC DC-derived to suppress DTH response was MHC class II and, part, Fas ligand/Fas dependent. were internalized by CD11c+ dermis at site injection...

Abstract Objective We have demonstrated previously that dendritic cells (DCs) modified with immunosuppressive cytokines, and exosomes derived from DCs can suppress the onset of murine collagen‐induced arthritis (CIA) reduce severity established arthritis. Indoleamine 2,3‐dioxygenase (IDO) is a tryptophan‐degrading enzyme important for immune regulation tolerance maintenance. expressing functional IDO inhibit T by depleting them essential tryptophan and/or producing toxic metabolites, as well...

Abstract Exosomes are 50- to 100-nm vesicles that formed within the late endocytic compartment and released from a variety of cell types. Previously, we demonstrated exosomes derived dendritic cells transduced with adenoviral vectors expressing IL-10, IL-4, or Fas ligand (FasL) produce anti-inflammatory able reduce inflammation in murine paw delayed-type hypersensitivity model, suppress onset on collagen-induced arthritis, severity established collagen-induce arthritis. In this study,...

Previous reports have demonstrated the ability of antigen-presenting cells (APCs), genetically modified to express Fas ligand (FasL), inhibit T-cell responses through induction apoptosis antigen-specific T cells. Here we examined primary mouse bone marrow-derived dendritic (DCs), by adenoviral infection FasL, progression established collagen-induced arthritis (CIA) in DBA/1 mice. Systemic injection DC/FasL into mice with CIA resulted substantial disease amelioration as determined analysis...

Abstract Costimulatory molecules, such as B7‐1/2 and PD‐L1/2 play an important role in the function of APC. The regulation surface levels costimulatory molecules is one mechanism by which APC maintain balance between tolerance immunity. We examined contributions to IL‐10‐treated, immunosuppressive DC well therapeutic exosomes derived from these DC. IL‐10 treatment significantly downregulated expression MHC II, B7‐1, B7‐2, decreased I PD‐L2. resulted a modified profile DC‐secreted with...

The nucleotide excision repair (NER) machinery removes UV photoproducts from DNA in the form of small, excised damage-containing oligonucleotides (sedDNAs) ∼30 nt length. How cells process and degrade these byproducts is not known. Using a small scale RNA interference screen UV-irradiated human cells, we identified TREX1 as major regulator sedDNA abundance. Knockdown increased level sedDNAs containing two their association with NER proteins TFIIH RPA. Overexpression wild-type but...

ABSTRACT Murine leukemia virus (MLV)-based retroviral vectors are the most frequently used gene delivery vehicles. However, current still not fully optimized for expression and viral titer, many genetic biochemical features of MLV-based poorly understood. We have previously reported that vector MFG, where interest is expressed as a spliced mRNA, superior in level with respect to other compared study. As one approach developing improved vectors, we systematically performed mutational analysis...

Intra-articular expression of antagonists interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in arthritic rabbit knee mouse ankle joints by direct adenoviral-mediated intraarticular delivery results amelioration disease pathology both the treated contralateral untreated joints. Previous experiments suggest that adenoviral infection resident antigen-presenting cells (APCs) subsequent traveling these to other sites inflammation lymph nodes might be responsible for this “contralateral...

Anesthetic management of critical airway stenosis is often very challenging. Extracorporeal membrane oxygenation (ECMO) may provide adequate respiratory support when conventional approaches fail. We report our experience ECMO for surgery. Between April 2012 and March 2015, nine patients underwent ECMO-supported operation. The reason surgery was tracheal in patients, tracheomalacia, tumor, external compression by mediastinal mass one patient each. Resection end-to-end anastomosis performed...

Abstract Pulmonary contusion is an important risk factor for respiratory complications in trauma patients. Hence, we aimed to determine the relationship between ratio of pulmonary volume total lung and patient outcomes predictability complications. We retrospectively included 73 patients with a on chest computed tomography (CT) from 800 admitted our facility January 2019 2020. Chest injury severity was expressed as by quantifying CT. The cut-off value 80%. Among (77% males, mean age: 45.3...

Document authentication is one of the most important fields in forensic science and includes analysis handwriting, seal imprint, printed materials. In this study, we investigated intersection lines by pen inks, red stamping ink, laser toner using time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) attenuated total reflectance Fourier transform infrared spectroscopy (ATR FTIR). TOF‐SIMS very useful when quantity sample insufficient or precious. Each ink showed specific peaks from...

In patients with tracheal injuries, conservative treatment is an alternative approach when surgical difficult. However, the success rate of low a ventilator used constantly because underlying lung disease, and successful requires maintenance as much self-respiration possible without ventilator. Here, we report case lower injury in which both treatments were difficult, but extracorporeal membrane oxygenation was while maintaining

Sphingopyxis sp. strain MWB1, which is capable of degrading crude oil, diesel, and kerosene, was isolated from oil-contaminated seashore in Tae-an, South Korea. Here, we report the draft genome sequence this strain, comprises 3,118,428 bp with a G+C content 62.85 mol%.

<i>Background:</i> Allergic reactions to food can involve diarrhea, vomiting, nausea and abnormal pain. PG102 has previously been shown control various factors involved in allergy pathogenesis, including IgE Th1 Th2 cytokines, vivo as well vitro [Park EJ, et al.: J Allergy Clin Immunol 2005;116:1151–1157; Park Invest Dermatol 2007;127:1154–1160]. These data indicate that might have antiallergic effects on allergic diarrhea. Here, we investigated whether could prevent diarrhea the...

Several factors associated with referral time to hospice and/or palliative care services have been identified, but there is no literature on the association between these and emotional status of family caregivers (FCs). This article intended address that issue.A semistructured interview was employed collect data for a retrospective cohort study. The primary FCs terminally ill cancer patients were interviewed at patient's unit. Interview combined patients' medical record our analysis....

As part of our current projects to discover biologically active compounds from natural sources, we conducted a phytochemical investigation Juncus decipiens, species generally distributed throughout Korea. J. decipiens has been used in traditional Chinese medicine control diuresis for strangury and clear out heart fire. The the EtOH extract led isolation five phenolic (1–5) via semi-preparative HPLC purification. chemical structures isolated were identified as isosaponarin (1), isovitexin...

Background/Objectives: The ectodysplasin A (EDA) gene, a member of the tumor necrosis factor ligand superfamily, is involved in early epithelial–mesenchymal interaction that regulates ectoderm-derived appendage formation. Numerous studies have shown mutations EDA gene can cause X-linked ectodermal dysplasia (ED) and non-syndromic oligodontia (NSO). Accordingly, this study aimed to identify causative genetic gene. Methods: We investigated two families using candidate sequencing whole-exome...

The effect of prepolymer structure on the electro-optic properties polymer dispersed liquid crystals (PDLCs) prepared from UV-cured polyurethane acrylates (PUAs) has been studied. Among four types films with different soft-segment structure, polypropylene glycol (PPG)- and polytetramethyl ether (PTMG)-based PUAs gave lower threshold (V10) operating (V90) voltages, whereas polycaprolactone diol (PCL)-based higher V10 V90 voltages. use mixed polyol glycol/ poly(tetramethylene adipate)diol)...