A5074893458- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Cell death mechanisms and regulation
- 3D Printing in Biomedical Research
- Telomeres, Telomerase, and Senescence
- Algal biology and biofuel production
- Cellular Mechanics and Interactions
- interferon and immune responses
- Carcinogens and Genotoxicity Assessment
- Cardiomyopathy and Myosin Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Tissue Engineering and Regenerative Medicine
- Growth Hormone and Insulin-like Growth Factors
- RNA Interference and Gene Delivery
- Connective tissue disorders research
- Atmospheric aerosols and clouds
- Infrared Target Detection Methodologies
- Radiative Heat Transfer Studies
- Sports injuries and prevention
- Pesticide and Herbicide Environmental Studies
- Protein purification and stability
- Ultrasound and Cavitation Phenomena
- Single-cell and spatial transcriptomics
- Chronic Kidney Disease and Diabetes
Stanford University
2022-2024
Yeungnam University
2001-2022
Korea Research Institute of Standards and Science
2012-2022
Korea University of Science and Technology
2016-2022
Inha University
2022
Honam University
2022
Korea University
2022
Park University
2022
Yeungnam University College
2021
Daegu Gyeongbuk Institute of Science and Technology
2020
Abstract Senescent cells accumulate in various tissues over time and contribute to tissue dysfunction aging-associated phenotypes. Accumulating evidence suggests that cellular senescence can be inhibited through pharmacological intervention, as well treatment with soluble factors derived from embryonic stem (ESCs). In an attempt investigate the anti-senescence secreted by ESCs, we analyzed mouse ESC-derived extracellular microRNAs conditioned medium via microRNA array analysis. We selected...
High-level expression of recombinant human growth hormone (hGH) in Escherichia coli (E. coli) leads to the formation insoluble aggregates as inclusion bodies devoid biological activity. Until recently, significant efforts have been made improve recovery active hGH from bodies. Here, we developed an efficient procedure for production completely soluble by minimizing and optimizing protein purification conditions. Under newly established conditions were able obtain most total fraction. We show...
The nucleotide excision repair (NER) machinery removes UV photoproducts from DNA in the form of small, excised damage-containing oligonucleotides (sedDNAs) ∼30 nt length. How cells process and degrade these byproducts is not known. Using a small scale RNA interference screen UV-irradiated human cells, we identified TREX1 as major regulator sedDNA abundance. Knockdown increased level sedDNAs containing two their association with NER proteins TFIIH RPA. Overexpression wild-type but...
Abstract Vascular smooth muscle cells (VSMCs) have remarkable plasticity in response to diverse environmental cues. Although these are versatile, chronic stress can trigger VSMC dysfunction, which ultimately leads vascular diseases such as aortic aneurysm and atherosclerosis. Protein arginine methyltransferase 1 (Prmt1) is a major enzyme catalyzing asymmetric dimethylation of proteins that sources dimethylarginine (ADMA), an endogenous inhibitor nitric oxide synthase. potential role Prmt1...
Abstract Intimal sarcoma of the pulmonary artery is a rare malignant tumor that may be misdiagnosed as chronic thromboembolism, even if various imaging techniques are used. We report case 58-year-old man with intimal sarcoma. 18 F-fleuorodeoxyglucose (FDG) uptake was poor in mass artery, and no other hypermetabolic lesions were noted elsewhere. Our presumptive diagnosis massive mural thrombus concomitant thromboembolism. Intravenous heparin recombinant human tissue-type plasminogen activator...
Other SectionsAbstractINTRODUCTIONRESULTSDISCUSSIONCONCLUSIONMATERIALS AND METHODSACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences
Novel in vivo excision assays for monitoring the excised oligonucleotide products of nucleotide repair UV-irradiated cells have provided unprecedented views kinetics and genomic distribution events. However, an unresolved issue is fate their mechanism degradation. Based on our observation that decreases abundance coincide with induction apoptotic signaling cells, we considered possibility caspase-mediated contributes to degradation or a general inhibition system. genetic pharmacological...
Adozelesin is a synthetic analog of the antitumor antibiotic CC-1065, which alkylates N3 adenine in minor groove sequence-selective manner. Since cytotoxic potency DNA alkylating agent can be modulated by excision repair system, we investigated whether nucleotide (NER) and base (BER) enzymes are able to excise bulky adduct induced adozelesin. The UvrABC nuclease 3-methyladenine-DNA glycosylase, that exhibit broad spectrum substrate specificity, were selected as typical NER BER enzymes,...
It is necessary to understand movement characteristics of elite female soccer players during a match develop effective training program. The purpose this study analyze the physical demands Korean professional competitive season. Twenty-four (age: 27.8± 3.9 years; height: 165.7±5.1 cm) from team, belonging women’s league in South Korea participated study. 11 home matches and 10 away April 26 November 9 2011 Body weight body mass index decreased first half-season compared preseason...
Cell death-inducing DNA fragmentation factor 45 (DFF45)-like effector (CIDE) domains were initially identified as protein interaction modules in apoptotic nucleases and are now known to form a highly conserved family with diverse functions that range from cell death lipid homeostasis. In the fly, four CIDE domain-containing proteins (DFF-related [DREP]-1–4) their functions, including relationships, have been identified. this study, we introduced investigated acidic side-disrupted mutants of...