A5032617817- Microfluidic and Capillary Electrophoresis Applications
- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Bio-sensing Technologies
- Metabolism and Genetic Disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Muscle metabolism and nutrition
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Genetics, Bioinformatics, and Biomedical Research
- Electrowetting and Microfluidic Technologies
- Environmental DNA in Biodiversity Studies
- Pesticide Residue Analysis and Safety
- Molecular Sensors and Ion Detection
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Phylogenetic Studies
Korea Research Institute of Standards and Science
2013-2024
Korea University of Science and Technology
2011-2016
University of Utah
1998-2003
Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment many cancers. Such measurements will be fundamental in the future support precision medicine. However, there are currently no primary reference measurement procedures available nucleic acid quantification that would translation tests into routine use.We assessed accuracy digital PCR (dPCR) copy number a frequently occurring single-nucleotide variant colorectal cancer (KRAS c.35G>A,...
An on-column sample concentration method for capillary-based DNA sequencing was studied. This base-stacking allows direct injection of unpurified products dye-primer reactions onto capillaries without any pretreatment. On-column fragments is achieved simply by electrokinetic hydroxide ions. A neutralization reaction between these OH- ions and the cationic buffer component Tris+ results in a zone lower conductivity, within which field focusing occurs. are concentrated at front this...
The health of their populations and efficient care systems are critical importance to the economic social well-being nations. Accurate comparable peptide/protein measurements required in support diagnosis, prognosis, monitoring treatment widespread diseases (e.g. diabetes). consistency measurement results can be achieved by making them traceable stated references through development Reference Measurement Systems. review mainly concentrates on progress made Protein Analysis Working Group...
The performance of thermal cyclers for polymerase chain reactions (PCR) is great concern in terms the reliability PCR-based assays, particularly when rapid cycling conditions are applied to small volume reactions. In this work, precision temperature controls during was measured 19 commercial 8 different models. temperatures test solutions specific locations each block were simultaneously monitored at 1 s intervals cycling. A temperature-sensitive multiplex PCR run parallel assess undesirable...
Enumeration-based determination of DNA copy-concentration was assessed through an international comparison among national metrology institutes (NMIs) and designated (DIs). quantification does not require a calibration standard thereby providing route to “absolute quantification”, which offers the potential for reliable value assignments reference materials, International System Units (SI) traceability copy number 1 accurate counting. In this study, 2 enumeration-based methods, flow...
A novel sample pretreatment device is described, and its application to the concentration purification of crude DNA samples in a flowing stream for subsequent capillary electrophoresis demonstrated. The consists two gap junctions, each covered with conductive membrane material built upon flow channel made PEEK tubing. Upon an electric field between negatively charged fragments can resist hydrodynamic are trapped junctions. dissolved microliter volumes captured nanoliter-sized band by simply...
Abstract Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without help standards. However, it can generate false results when conditions are not optimized. A recent international comparison (CCQM P154) showed that most laboratories significantly underestimated concentration supercoiled plasmid by dPCR. Mostly, DNAs linearized before dPCR to avoid such underestimations. The present study was conducted overcome this problem. In...
Required metrological tools, such as higher order reference measurements procedures, pure substance and matrix certified materials, are established for small well defined molecules.Difficulties still remain in the provision of SI traceable standards area larger biomolecules peptides/proteins.The Primary Calibration Reference Services has been identified a core technical competency National Measurement Institutes (NMIs).A concept elaborated by Focus Group I on peptide/protein purity strategic...
We report a novel method for rapid quantification of the degree DNA methylation specific gene. Our combined bisulfite-mediated PCR and deoxyribonucleoside monophosphate (dNMP) contents in product through capillary electrophoresis. A bisulfite-PCR was enzymatically hydrolyzed to dNMP monomers which were quantitatively analyzed subsequent following bisulfite treatment converts unmethylated cytosines thymines while leaving methyl-cytosines unchanged. Then ratio cytosine thymine determined by...
Quantification of trace amounts DNA is a challenge in analytical applications where the concentration target very low or only limited samples are available for analysis. PCR-based methods including real-time PCR highly sensitive and widely used quantification low-level samples. However, ordinary require at least one copy specific gene sequence amplification may not work sub-genomic amount DNA. We suggest whole genome method adopting degenerate oligonucleotide primed (DOP-PCR) This approach...
Precise determination of ribonucleic acid (RNA) concentration without the need for calibration was pursued by sequence-specific counting individual RNA molecules. This approach eliminates reverse transcription (RT) step required polymerase chain reaction (PCR)-based quantification, which may hamper accurate measurements owing to uncertain yields RT reactions. Target RNAs were tagged with a number fluorescent oligonucleotide probes complementary sequences. Tagged exhaustively counted one...
Quantitation of the trace amount DNA by counting individual molecules using a high-sensitivity flow cytometric setup has been developed and evaluated for purpose establishing reference analytical procedure. Model molecules, represented lambda (λ) viral (48 502 bp, double-stranded), were electro-focused to form tightly bound stream on detection point situated centre axis fused silica tubing measuring 50 µm × 50 µm. The particles that stained with fluorescent dye detected individually...
Abstract Isotope dilution liquid chromatography/tandem mass spectrometry (ID‐LC/MS/MS) has been developed as a candidate reference method to determine the level of phenylalanine in human serum. The advantages this include simple sample preparation without derivatization, selective detection analytes, and use an isotopic analogue internal standard. Phenylalanine its analogue, phenylalanine‐ring‐ 13 C 6 , were monitored at transitions m/z 166.2/120.2 172.2/126.2 multiple‐reaction monitoring...