Sema Akyürek
A5008642387- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Genetically Modified Organisms Research
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- interferon and immune responses
- RNA modifications and cancer
- Geochemistry and Geologic Mapping
- CRISPR and Genetic Engineering
- Wheat and Barley Genetics and Pathology
- MicroRNA in disease regulation
- Geological and Geochemical Analysis
- Cancer Genomics and Diagnostics
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 detection and testing
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Radiation Effects and Dosimetry
- Advanced Biosensing Techniques and Applications
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Clay minerals and soil interactions
Tübitak National Metrology Institute
2012-2024
University of Mumbai
2022
National Institute of Metrology
2018
Scientific and Technological Research Council of Turkey
2018
Gebze Technical University
2018
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2016
Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment many cancers. Such measurements will be fundamental in the future support precision medicine. However, there are currently no primary reference measurement procedures available nucleic acid quantification that would translation tests into routine use.We assessed accuracy digital PCR (dPCR) copy number a frequently occurring single-nucleotide variant colorectal cancer (KRAS c.35G>A,...
Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being used to diagnose tuberculosis (TB). While qualitative methods adequate for diagnosis, therapeutic monitoring of TB patients requires quantitative currently performed using smear microscopy. The potential use molecular measurements has been investigated but findings have variable and inconclusive. lack an reference method materials is a barrier understanding source disagreement. Digital (dPCR) offers...
Quantitative PCR (qPCR) is an important tool in pathogen detection. However, the use of different qPCR components, calibration materials and DNA extraction methods reduces comparability between laboratories, which can result false diagnosis discrepancies patient care. The wider establishment a metrological framework for nucleic acid tests could improve degree standardisation detection quantification applied clinical context. To achieve this, accurate need to be developed implemented as...
Main text Nucleic acid amplification tests for SARS-CoV-2, the virus responsible COVID-19 pandemic, primarily target RNA as analyte. These detect presence of SARS-CoV-2 specific sequences, confirming infection through in vitro diagnostic methods. However, lack a standardized reference measurement system has led to varied units and unclear traceability reporting content quantities, complicating comparisons between different [1-5]. To address this challenge, pilot study CCQM-P199b was...
Measurement of RNA can be used to study and monitor a range infectious non-communicable diseases, with profiling multiple gene expression mRNA transcripts being increasingly applied cancer stratification prognosis. An international comparison (Consultative Committee for Amount Substance (CCQM)-P103.1) was performed in order evaluate the comparability measurements copy number ratio targets between two samples. Six exogenous synthetic comprising External Control Consortium (ERCC) standards...
Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested national metrology institutes (NMIs) designated (DIs) in determination relative quantity two specific genomic DNA fragments present a biological tissue. The study provides support for following measurement claim: "Quantification ratio number copies specified intact sequence length range 70 100 nucleotides single extract from ground maize seed materials".
ABSTRACT Nucleic acid amplification tests including reverse transcription quantitative PCR (RT-qPCR) are used to detect RNA from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19) pandemic. Standardized measurements can facilitate comparable performance laboratory in absence existing reference measurement systems early on a Interlaboratory study CCQM P199b “SARS-CoV-2 copy number quantification” was designed test fitness-for-purpose...
ABSTRACT Infection with human immunodeficiency virus (HIV)-1 leads to acquired syndrome (AIDS) if left untreated. According UN figures, approximately 39 million people globally were living HIV in 2022, 76% of those individuals accessing antiretroviral therapy. Measurement plasma viral RNA load using calibrated nucleic acid amplification tests (like reverse transcription quantitative PCR, RT-qPCR) is routinely performed monitor response treatment and ultimately prevent transmission....
SARS-CoV-2 in vitro transcribed RNA reference materials (RM), UME RM 2019 and 2020, were produced by Scientific Technological Research Council of Turkey (TUBITAK), National Metrology Institute (UME), to be used as a quality control material for measurements, liquid-frozen lyophilized forms, respectively. These RMs include ten internationally recommended target gene fragments (Pasteur-RdRp-IP2, Pasteur-RdRp-IP4, Charite-E, Charite-RdRp, CDC-N1, CDC-N2, China CDC-ORF1ab, CDC-N, Hong...
A key comparison CCQM-K86.b was performed to demonstrate and document the capacity of National Metrology Institutes (NMIs) Designated (DIs) in determination relative quantity two specific genomic DNA fragments present a rice powder. The study provides support for following measurement claim: "Quantification ratio number copies specified intact sequence length up 150 nucleotides extraction from an unprocessed, high starch ground seed matrix, with copy 0.005 1". carried out under auspices...
In this study, we provide a method using fluorescently labeled oligonucleotides for the diagnosis of microorganisms producing nucleases in real time, while growing them culture media. The detection such was possible short period as 10 minutes up to maximum 8 hours, depending on bacterial density. We also showed suitability new determination minimum inhibitory concentration (MIC) media very compared conventional methods. believe that it can make significant contribution gain insights analysis...
Dogu Karadeniz Bolgesi'nde, Kibletepe (Arakli, Trabzon) ve cevresindeki volkanik kayaclar Gec Kretase yasli olup, tabanda bazalt, andezit piroklastlari, bunlarin ustundeki dasit, riyolit piroklastlari ile bunlari kesen bazalt dayklarindan olusmaktadir. dasitik riyolitik hyalo-porfirik sferulitik dokuda baslica plajiyoklas, kuvars hornblend minerallerinden Cevherlesmesi agsal, sacinimli bresik yapida bulunmaktadir. Pirit, sfalerit, kalkopirit daha az oranda fahlerz, dijenit, kovellin malahit...
BACKGROUND AND AIM: Organ�c-�norgan�c hybr�d nanofthey have h�gh stab�l�ty, low product�on cost, cheaper pur�f�cat�on processes, overcom�ng substrate/product �nh�b�t�on and easy recovery.We synthes�zed tr�ple enzyme (amylase, l�pase, pro-Cu(II)) tested the�r ant�m�crob�al act�v�ty �n compar�son w�th ch�tosan.MATERIALS METHODS: We ch�tosan from sh-r�mp shells (TrpE@Cu(II)) us�ng amylase, protease enzymes.SEM analys�s, thermograv�metr�c analys�s (TGA), the degree of deacetylat�on (%DD) were...