Magnetic reconnection requires the violation of frozen-in condition which ties gyrating charged particles to magnetic field inhibiting diffusion. Ongoing has been identified in near-Earth space as being responsible for excitation substorms, storms, generation aligned currents and their consequences, wealth auroral phenomena. Its theoretical understanding is now on verge completed. Reconnection takes place thin current sheets. Analytical concepts proceeded gradually down microscopic scale,...

Cholesterol influences ion-channel function, distribution and clustering in the membrane, endocytosis exocytic sorting of nicotinic acetylcholine receptor (AChR). We report occurrence a cholesterol recognition motif, here coined "CARC", transmembrane regions AChR subunits that bear extensive contact with surrounding lipid are thus optimally suited to convey cholesterol-mediated signaling from latter. Three molecules could be docked on segments each subunit, rendering total 15 per molecule....

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of lipids on acetylcholine receptor. Essential need cholesterol for maintenance agonist-induced state transitions in lipid vesiclesM. Criado, H. Eibl, and F. J. BarrantesCite this: Biochemistry 1982, 21, 15, 3622–3629Publication Date (Print):July 20, 1982Publication History Published online1 May 2002Published inissue 20 July 1982https://doi.org/10.1021/bi00258a015RIGHTS & PERMISSIONSArticle Views167Altmetric-Citations145LEARN ABOUT THESE...

The lipid environment of acetylcholine receptor-rich membranes from Torpedo marmorata has been studied with spin labels. electron resonance spectra both stearic acid and steroid probes in the revealed an immobilized component, addition to fluid component which is found aqueous bilayer dispersions extracted lipids. labels also cause a differential paramagnetic quenching intrinsic protein fluorescence membranes, sensitive action cholinergic ligands follows modified Stern-Volmer law. Electron...

Torpedo acetylcholine receptor was reconstituted into liposomes of pure synthetic lipids in order to study the influence lipid environment on affinity state transitions and ion translocation function receptor. A critical concentration 30 40% cholesteryl hemisuccinate necessary made dimyristoyl phosphatidylcholine mimic kinetics agonist-induced observed native membranes. With increasing chain length saturated lecithins, a marked increase carbamylcholine dissociation constants observed....

Abstract Cholesterol controls the activity of a wide range membrane receptors through specific interactions and identifying cholesterol recognition motifs is therefore critical for understanding signaling receptor function. The membrane-spanning domains paradigm neurotransmitter acetylcholine (AChR) display series consensus (referred to as “CARC”). Here we use combination molecular modeling, lipid monolayer/mutational approaches NMR spectroscopy study binding synthetic CARC peptide....

Nicotinic acetylcholine receptors (nAChRs) contribute significantly to hippocampal function. Alpha7-nAChRs are present in presynaptic sites neurons and may influence transmitter release, but the factors that determine their localization unknown. We report here Wnt-7a, a ligand active canonical Wnt signaling pathway, induces dissociation of adenomatous polyposis coli (APC) protein from beta-catenin cytoplasmic complex interaction APC with alpha7-nAChRs neurons. Interestingly, Wnt-7a...

An instrument is described in which two solutions can be homogeneously mixed within several microseconds. The liquids flow separately through coaxial capillaries with conical tips and then simultaneously around a sphere (50–100 μ diameter) has been positioned close to the end of outer tip. velocities ∼100 m/s small passages (∼5 wide) separating wall capillary mix turbulent liquid behind sphere. are ejected as narrow jet for observation. Design characteristics construction techniques...

Planar lipid bilayers were formed with the mixed chain phospholipid 1-stearoyl-3-myristolglycero-2-phosphocholine. Acetylcholine receptor membrane fragments or purified protein was incorporated into these by fusing receptor-containing vesicles planar membranes a few degrees below phase transition temperature. Single-channel currents activated nicotinic agonists in reconstituted system resembled those observed intact rat and frog muscle as measured patch clamp technique. The channel...

Abstract: When rat retinal cells are cultured in a serum‐free medium, the photoreceptor start dying after 7 days. The addition of docosahexaenoic acid (DHA) to cultures prevents selective death photoreceptors. Here it is shown that, unlike other neurons, photoreceptors die through an apoptotic pathway. Hallmarks apoptosis, such as nuclear fragmentation and condensation DNA cleavage forming ladder pattern on agarose gel, were observed. timing high selectivity triggering cell apoptosis suggest...

Abstract: The effect of docosahexaenoic acid (DHA) on neuronal survival was studied in cultured cells isolated from newborn rat retina. In vivo, the content DHA retina increased nearly fourfold days 2 to 12 after birth, whereas retinal culture it remained constant. Unlike amacrine cells, photoreceptor control cultures underwent a selective degeneration, starting at day 7, that led their massive death by 11. addition 7 its active incorporation cultures, increasing 6 21% total fatty acids cell...