In this study 98 rhizospheric actinomycetes were isolated from different wheat and tomato fields, Punjab, Pakistan. The isolates characterized morphologically, biochemically, genetically subjected to a comprehensive in vitro screening for various plant growth promoting (PGP) traits. About 30% of the screened found be promising PGP rhizobacteria (PGPRs), which exhibited maximum genetic similarity (up 98-99%) with species genus Streptomyces by using16S rRNA gene sequencing. most active indole...

5-Fluorouracil (5-FU) being a mainstream anticancer drug is under keen and detailed investigation for prodrugs formulations in order to minimize the associated side effects. Cocrystallization of 5-FU an innovative technique synthesis improve its effectiveness. The present study based on supramolecular synthons with four coformers: succinic acid, cinnamic malic benzoic acid utilizing acetone as solvent. Solid state grinding followed by slow evaporation solution method was applied. Colorless...

Hydrogel‐based drug delivery systems can leverage therapeutically favorable upshots of release and found clinical uses. Hydrogels offer temporal spatial control over the different therapeutic agents. Because their tailor made controllable degradability, physical properties, ability to prevent labile drugs from degradation, hydrogels provide platform on which diverse physicochemical interactions with entrapped cause release. Herein, we report fabrication novel vinyltrimethoxy silane (VTMS)...

Magnetic cores loaded with metallic nanoparticles can be promising nano-carriers for successful drug delivery at infectious sites. We report fabrication, characteristic analysis and in vitro antibacterial performance of nanocomposites comprising cobalt (Co-cores) functionalized a varied concentration silver (AgNPs). A two-step polyol process synchronized the transmetalation reduction method was used. Co-cores were synthesized acetate, decoration AgNPs carried out acetate. The density by...

This study reports the formation of 5-FU co-crystals with four different pharmacologically safe co-formers; Urea, Thiourea, Acetanilide and Aspirin using methanol as a solvent. Two fabrication schemes were followed i.e., solid-state grinding protocol, in which API co-formers mixed through vigorous while other method separate solutions both components made together. The adopted approaches offer easy protocols, no temperature maintenance requirements, need expensive solvents, hardly available...

Background. Enterococci are a part of the natural intestinal flora humans and animals play an important role in keeping microbial balance gut. Many species also used as probiotics that produce vitamins, stimulate immune response, maintain integrity The use dietary supplements to reinforce some gut components is current aspect functional food sciences treat various diseases. Methodology. In study, 21 Enterococcus strains were isolated identified morphologically, biochemically,...

Abstract This study reports the prevalence and antibiotics resistance status of Salmonella detected in raw meat from Lahore, Pakistan. Overall, N = 111 samples, were collected local markets. was recovered 57 (51.35%) including 45.83% poultry, 60% buffalo, 64.28% cow, goat samples. The predominant strains enterica serovars; Typhimurium (45.4%), Typhi (27.2%), Enteritidis (18.1%), identified by VITEK system 16S rRNA gene sequencing. isolates exhibited high to Erythromycin (100%), Cefepime...

The structures and bioactivities of three unprecedented fused 5-hydroxyquinoxaline/alpha-keto acid amino metabolites (baraphenazines A–C, 1–3), two unique diastaphenazine-type D E, 4 5) new phenazinolin-type F G, 6 7) from the Himalayan isolate Streptomyces sp. PU-10A are reported. This study highlights first reported bacterial strain capable producing diastaphenazine-type, phenazinolin-type, izumiphenazine A-type presents a opportunity for future biosynthetic interrogation late-stage...

Pakistan is blessed with plants of Asteraceae family known medicinal background used for centuries by Hakims (traditional physicians). Keeping in mind the their anti-larval potential, a total 21 endophytic actinomycetes were isolated from four and screened against first fourth instar stages Culex quinquefasciatus Say mosquito larvae. Of isolates, 6 them gave strong larvicidal activity (80–100% mortality) screening results 4 isolates potent (100% at stage. These belonged to different species...

Actinomycetes are gram positive bacteria with high G + C content in their DNA and capable of producing variety secondary metabolites. Many these metabolites possess different biological activities have the potential to be developed as therapeutic agents. The aim present study was screen actinomycetes inhabiting halophilic environment such Khewra salt mines Pakistan for cytotoxic antitumor compounds. An actiomycetes strain designated Streptomyces sp. KML-2 isolated from a saline soil mines,...

Actinobacteria are famous for the production of unique secondary metabolites that help in controlling continuously emerging drug resistance all over globe. This study aimed at investigation an extreme environment Cholistan desert, located southern Punjab, Pakistan, actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The desert is a sub-tropical arid ecosystem with harsh environment, limited rainfall low humidity. 20 soil sand samples were...

Bioprospecting of actinobacteria isolated from Kubuqi desert, China for antibacterial, antifungal and cytotoxic metabolites production their structure elucidation.A total 100 strains were selectively Inner Mongolia, China. The taxonomic characterization revealed Streptomyces as the predominant genus comprising 37 different species, along with rare actinobacterial Lentzea. methanolic extracts 60.8% exhibited potent antimicrobial activities against Staphylococcus aureus, Micrococcus luteus,...

An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor miehei and Candida albicans in pre-screening studies, was investigated by cultivation a 50-L fermenter subsequent isolation, purification, structure elucidation of the active metabolites. Based on morphological, biochemical, physiological characterization, as well 16S rRNA gene sequence, CTF9 identified malachitofuscus. Using series chromatographic techniques, two pure compounds were isolated from...

The isolation and structure elucidation of four new naturally occurring amino-nucleoside [puromycins B–E (1–4)] metabolites from a Himalayan isolate (Streptomyces sp. PU-14-G, isolated the Bara Gali region northern Pakistan) is reported. Consistent with prior reports, comparative antimicrobial assays revealed need for free 2″-amine anti-Gram-positive bacteria antimycobacterial activity. Similarly, cancer cell line cytotoxicity highlighted importance puromycin-free impact 3′-nucleoside...

Synthesis of some novel 1-(3,4,5-trimethoxy)benzoyl-3- arylthiourea derivatives (1a-o) was accomplished in 2 steps. The synthetic route involves the reaction 1-(3,4,5-trimethoxy)benzoyl chloride with potassium thiocyanate 1:1 molar ratio acetone to afford corresponding isothiocyante followed by treatment suitably substituted anilines. structures products were established elemental analyses, IR, ^1H- and ^{13}C-NMR, mass spectroscopy for 1b 1m from single crystal X-ray diffraction data. All...

Actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. This study sought assess the ability selected actinomycetes control biofilm forming isolated from different dental plaque samples. On basis morphological differences three out ten bacterial isolates were further study. These biochemically and genetically characterized identified as Acinetobacter schinndleri, Moraxella aci, Bacillus cereus. Antibiotic resistant profile was measured through...