Extensive use of chromium (Cr) and arsenic (As) based preservatives from the leather tanning industry in Pakistan has had a deleterious effect on soils surrounding production facilities. Bacteria have been shown to be an active component geochemical cycling both Cr As, but it is unknown how these compounds affect microbial community composition or prevalence form metal resistance. Therefore, we sought understand effects that long-term exposure As diversity structure soil communities. Soils...

Drought accompanied with reduced precipitation is one of the key manacles to global agricultural throughput and expected escalate further hence posing major challenges future food safety. For a sustainable environment, drought resistant plant growth promoting rhizobacteria (PGPR) are new encouraging prospect, which inexpensive have no side effects, as those synthetic fertilizers. In present study, five strains Pseudomonas aeruginosa, strain MK513745, MK513746, MK513747, MK513748, MK513749...

The aim of this study was to investigate the potential bacterial strains Bacillus, Pseudomonas, Escherichia, Micrococcus and Staphylococcus genera associated with wild herbaceous flora enhance endogenous indole-3-acetic acid (IAA) content growth Triticum aestivum var. Inqalab-91.Gas chromatography mass spectrometric (GC-MS) analysis revealed that produced 0.6-8.22 microg IAA ml(-1) in presence L-tryptophan. Plant microbe experiments showed a significant positive correlation between auxin...

Plant-associated bacteria are known to improve plant growth and play a major role in the development of plants. The present study is concerned with isolation two auxin-producing growth-promoting (PGPB). On basis 16S rRNA sequencing, both strains identified as Bacillus sp. Maximum auxin production was observed at 37 °C after 48 h incubation. Increase tryptophan concentration stimulated by isolates. High-performance liquid chromatography analysis showed that bacterial exhibited similar...

The growing human population and abiotic stresses pose significant threats to food security, with PGPR favorable as biofertilizers for plant growth stress relief. In one study, soil samples from both cultivated uncultivated plants in various cities were used isolate rhizobacterial populations. Using 50 plants, isolated populations screened biochemical changes, PGP activities morphological characteristics. A total of 199 rhizobacteria IAA production. strain M28 produced maximum 378.44 ± 2.5...

Small circular single-stranded DNA satellites, termed DNAβ, have recently been found associated with some geminivirus infections. The β Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan. Mutagenesis revealed that the complementary-sense open reading frame (ORF) βC1 required inducing symptoms Nicotiana tabacum. An ORF present on virion-sense strand βV1 appeared to no role pathogenesis. Tobacco plants transformed under control Cauliflower mosaic...

Plant growth promoting rhizobacteria (PGPR) promote plant by various mechanisms such as phytohormone production, enhanced water and nutrient uptake, improved nitrogen availability in the soil, production of ACC-deaminase for ethylene breakdown, phosphate solubilization, siderophore etc. Microbial auxin is major factor not only responsible strengthening plant-microbe relationship but it also promotes development a positive manner. Thus, bacterial potential can be exploited improvement that...

Four non‐heterocystous cyanobacterial strains isolated from rhizosphere and soil surface mat of rice, wheat, maize were identified by 16S rRNA gene sequencing evaluated for auxin production as well growth promotion wheat. Isolated produced a diverse amount in BG11 media supplemented with different concentrations L ‐tryptophan. The detected colorimetric methods was higher compared to GC–MS analysis. Auxin BG 11 medium 250 µg ml −1 ‐tryptophan ranged 0.20 1.63 IAA revealed gas chromatography...

The nucleotide sequence of a 2.7-kilobase segment DNA containing the sdhA and sdhB genes encoding flavoprotein (Fp, sdhA) iron-sulfur protein (Ip, sdhB) subunits succinate dehydrogenase Bacillus subtilis was determined. This extends previously reported cytochrome b558 subunit (sdhC) completes sdh operon, sdhCAB. predicted molecular weights for Fp Ip subunits, 65,186 (585 amino acids) 28,285 (252 acids), agreed with values determined independently labeled antigens, although it appeared that...

Commercial medium (Pharmamedia) was investigated for the production of surfactin by Bacillus subtilis MZ-7. Different media (defined, semi-defined, and complex media) were compared after fixing least influential variables in standardized fermentation conditions. Carbohydrate nitrogen supplements also tried to improve Pharmamedia.Surfactin confirmed using PCR along with other analytical techniques monitored RP-HPLC MALDI-TOF-MS. We found that optimized brain heart infusion best (280 mg/L) a...

Abstract In the present study antimicrobial potential of various extracts from 12 medicinal plants has been investigated in vitro on multiple antibiotic resistant pathogens and some selected protozoa isolated poultry. The initial examination was performed E. coli ( n = 10) using disc agar well diffusion assays. Only two plants, Peganum harmala (seeds) Eucalyptus globulus (leaves) showed positive responses. active were also against an additional 19 bacteria clonal cultures three Histomonas...

Congenital leptin deficiency is a rare human genetic condition clinically characterized by hyperphagia and acute weight gain usually during the first postnatal year. The worldwide data on this disorder includes only 14 cases four pathogenic mutations have been reported in gene.The objectives of study were to measure serum levels obese children detect gene those found be deficient.A total 25 recruited for study. Leptin was detected nine them. sequencing identified homozygous state all...

Bacterial strains isolated from rhizosphere of different plants were screened for cytokinin production by developing a simple and rapid bioassay technique. The assay was based on the ability to stimulate greening in etiolated cucumber cotyledons. Isolated bacterial grown half 0.6% agar plate 96 h at 30°C. Etiolated cotyledons placed 2 mm distance culture under green light. After initial incubation 20 darkness plates exposed light three hours. Greater chlorophyll contents obvious to Bacillus...

Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid-liquid extraction, acid ammonium sulfate precipitation followed by TLC, SPE, gel filtration. Active fractions further purified using RP-HPLC. The molecular mass product from HPLC determined through Tris-Tricine SDS-PAGE MALDI-TOF-MS. results revealed that produced surfactin iturin like compounds. Coproduction compounds this is a remarkable...