A5038368566- Chemical Synthesis and Analysis
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Structural Characterization
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Click Chemistry and Applications
- Enzyme Catalysis and Immobilization
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- Intramuscular injections and effects
- Cannabis and Cannabinoid Research
- Mosquito-borne diseases and control
- Systemic Lupus Erythematosus Research
- Neuroscience and Neuropharmacology Research
- Hepatitis C virus research
- Industrial Technology and Control Systems
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Analytical Chemistry and Chromatography
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Sexual Differentiation and Disorders
- Viral Infections and Vectors
- Pancreatic function and diabetes
University of Bristol
2019-2021
University of Glasgow
2021
Brigham Young University
2017
Icahn School of Medicine at Mount Sinai
2014
The Francis Crick Institute
2009
Augusta University
2008
MEI Research (United States)
2005
Royal Shrewsbury Hospital
1994
Abstract Subunit vaccines use delivery platforms to present minimal antigenic components for immunization. The benefits of such systems include multivalency, self‐adjuvanting properties, and more specific immune responses. Previously, the design, synthesis, characterization self‐assembling peptide cages (SAGEs) have been reported. In these, de novo peptides are combined make hubs that assemble into nanoparticles when mixed in aqueous solution. Here it is shown SAGEs nontoxic particles with...
COVID-19 is caused by a novel coronavirus called severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2). Virus cell entry mediated through protein-protein interaction (PPI) between the SARS-CoV-2 spike protein and angiotensin-converting enzyme (ACE2). A series of stapled peptide ACE2 peptidomimetics based on motif were designed to bind S-protein RBD inhibit binding human receptor. The assessed for antiviral activity in an array assays including neutralization pseudovirus assay,...
A single-stranded DNA binding protein (SSB), labeled with a fluorophore, interacts (ssDNA), giving 6-fold increase in fluorescence. The is the adduct of G26C mutant homotetrameric SSB from Escherichia coli and diethylaminocoumarin {N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide}. This can be used to assay production ssDNA during separation double-stranded by helicases. To use this probe effectively, as well investigate interaction between SSB, fluorescent has been develop...
The progress of total chemical protein synthesis has been hampered by difficulties in preparing peptide thioesters standard Fmoc synthesis. amino acid, α-methylcysteine, sited at the C-terminus a can substitute for thioester ligation reactions. C-terminal α-methylcysteine is fully compatible with and its use very simple robust. Its potential demonstrated model proteins.
B. Sedgwick, C. Morris and S. J. French, Chem. Soc., Commun., 1978, 193 DOI: 10.1039/C39780000193
During the summer of 1964 and spring 1965, California encephalitis group viruses were isolated from six pools boreal Aedes mosquitoes collected in near Rochester (54°N, 113°W), Alberta, Canada. Mosquitoes by aspiration, sweep-netting, with a CO2-baited cone trap. Collections placed on Dry Ice shipped to Madison, Wisconsin, for identification isolation virus. accounted 97% 16,453 examined Snowshoe hare virus was three communis group, one pool stimulans group. Jamestown Canyon A. spp. The...
It is well-documented that codon usage biases affect gene translational efficiency; however, it less known if viruses share their host's motifs.We determined human-infecting similar as proteins are expressed in tissues the infect.By performing 7,052,621 pairwise comparisons of genes from humans versus 113 infect humans, we which motifs were most highly correlated.We found 16 averaged a significant correlation with over 500 human per viral gene, 58 correlated an average at least 100 and 37...
Reduction of the trans-2-enoyl intermediate (1) to give saturated alkyl thioester (2) in fatty acid biosynthesis occurs by means an anti-addition hydrogen pro-2S and pro-3S position carbons forming double bond.
We report a simplified procedure for the chemical ligation of peptides by using sulfamylbutyryl linker as mildly activating group capable participating in ligation. When peptidyl N-methylsulfonamide is directly added with excess thiols to reactions, speed reaction comparable native
The orphan nuclear receptor DAX-1 (dosage-sensitive sex reversal-AHC critical region on the X chromosome gene 1; NR0B1) is known for its role in human development, specifically determination and steroidogenesis. Several recent publications have described an alternatively spliced form of called DAX-1A (NR0B1A). encoded by exons 1 2A DAX-1, with exon located within intron 1. expression has been observed several tissues, including adrenal gland, ovary, testis. Transfection studies further shown...
SARS-CoV-2 viral attachment and entry into host cells is mediated by a direct interaction between spike glycoproteins membrane bound angiotensin-converting enzyme 2 (ACE2). The receptor binding motif (RBM), located within the S1 subunit of protein, incorporates majority known ACE2 contact residues responsible for high affinity associated virulence. Observation existing crystal structures domain (S RBD )–ACE2 interface, combined with peptide array screening, allowed us to define series linear...
Abstract The design and assembly of peptide based materials has advanced considerably, leading to a variety fibrous, sheet nanoparticle structures. A remaining challenge is account for control different possible supramolecular outcomes accessible the same or similar building blocks. Here we present de novo system that forms nanoparticles sheets depending on strategic placement ‘disulfide pin’ between two elements secondary structure drive self-assembly. Specifically, join homodimerizing...