Endophytes constitute plant-colonizing microorganisms in a mutualistic symbiosis relationship. They are found most ecosystems reducing plant crops' biotic and abiotic stressors by stimulating immune responses, excluding pathogens niche competition, participating antioxidant activities phenylpropanoid metabolism, whose activation produces defense, structural support, survival molecules. In fact, metabolomic studies have demonstrated that endophyte genes associated to specific metabolites...

Abstract Ion mobility spectrometry (IMS) is routinely used in screening checkpoints for the detection of explosives and illicit drugs but it mainly relies on capture particles a swab surface detection. Solid phase microextraction (SPME) has been coupled to IMS preconcentration their volatile chemical markers and, although improved LODs over standalone IMS, limited sampling small vessels by fiber geometry. Novel planar geometry SPME devices coated with PDMS sol–gel that do not require an...

Abstract Pesticide resistance represents a major challenge to global food production. The spread of alleles is the primary explanation for observations reduced pesticide efficacy over time, but potential gene-by-environment interactions (plasticity) mediate susceptibility has largely been overlooked. Here we show that nutrition an environmental factor affects Bt toxins. Protein and carbohydrates are two key macronutrients insect herbivores, polyphagous pest Helicoverpa zea self-selects...

Helicobacter pylori is an infectious agent that colonizes the gastric mucosa of half population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by World Health Organization for role in development gastritis, peptic ulcers, and cancer. Due increase resistance antibiotics used anti-H. therapy, effective vaccine alternative great interest, which remains a challenge. Therefore, rational, strategic, efficient design against H. necessary where use most current...

Medicinal plants are traditionally used in Mexico to treat diseases such as cancer. The present study aimed evaluate the cytotoxic, antioxidant, and anti-hemolytic activity of 15 ethnopharmacological use Mexico. For this, plant methanol extracts were prepared by Soxhlet method, after which their cytotoxic was evaluated against human hepatocellular carcinoma (HEP-G2) monkey kidney epithelial (Vero) cells 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction colorimetric...

Background Bacillus genus has been used in horticultural crops as a biocontrol agent against insect pests, microbial phytopathogens, and plant growth-promoting bacteria (PGPB), representing an alternative to agrochemicals. In particular, B. cereus (Bc) thuringiensis (Bt) have studied for their fungicidal insecticidal activities. However, use biofertilizer formulations agents phytopathogenic is limited. Objective To evaluate Bc Bt PGPB the bacterial spot Xanthomonas euvesicatoria (Xe)...

Cancer is a major health problem with significant morbidity and mortality. In addition, plants are source of metabolites diverse biological properties, including antitumor potential. this study, we investigated the in vitro murine lymphoma L5178Y-R cell growth inhibition, human peripheral blood mononuclear cells (PBMC) toxicity proliferation, antioxidant, hemolytic, anti-hemolytic activities methanol extracts from 15 traditional use Mexico. Justicia spicigera caused highest tumor inhibition...

Identification of new sources compounds with anticancer and antioxidant potential is essential for cancer treatment. Rhizospheric bacteria have emerged as a promising source these agents. This study aimed at investigating the properties isolated from rhizosphere Lophocereus marginatus . Six rhizobacteria L. were molecularly identified by sequencing 16S rRNA gene MALDI‐TOF. We evaluated inhibitory activity methanol (MeOH) ethyl acetate (EtOAc) extracts obtained against murine lymphoma cell...

Glioblastoma is the most common and aggressive brain tumor in adults. However, due to limitations of conventional treatments, as well their side effects, there a need develop more effective less harmful therapy strategies. There evidence that plants endemic northern Mexico possess biological activities positively impact human health, particularly against cancer. Echinocereus engelmannii E. pectinatus are cacti from north produce bioactive compounds with antitumor activity. We obtained...

Current ion mobility spectrometry (IMS) devices are used to detect drugs and explosives in the form of particles and, cases where vapor pressure or is sufficiently high, gas can be sampled detected directly. The aim this study demonstrate use solid phase microextraction (SPME) as a preconcentration technique coupled an IMS for detection odor signature compounds explosives. reduced mobilities (K(o)) operating conditions cocaine, marijuana, 3,4-methylenedioxy-N-methylamphetamine (MDMA)...

Bacterial pathogens are a leading cause of waterborne disease, and may result in gastrointestinal outbreaks worldwide. Inhabitants the Bassaseachic Falls National Park Chihuahua, Mexico show seasonal gastroenteritis problems. This aim this study was to detect enteropathogenic microorganisms responsible for diarrheal area. In 2013, 49 surface water samples from 13 selected sampling sites along Basaseachi waterfall its main rivers, were collected during spring, summer, autumn, winter seasons....

Cancer cases result in 13% of all deaths worldwide. Unwanted side effects patients under conventional treatments have led to the search for beneficial alternative therapies. Microalgae synthesize compounds with known vitro and vivo biological activity against different tumor cell lines. Therefore, native microalgae from State Nuevo Leon, Mexico may become a potential source antitumor agents. The aim present study was evaluate cytotoxic effect Leon regional Chlorella sorokiniana...

Nuclear polyhedrosis viruses such as the one isolated from celery looper, Anagrapha falcifera (Kirby) (AfMNPV), have potential to be successful bioinsecticides if improved formulations can prevent rapid loss of insecticidal activity environmental conditions sunlight and rainfall. We tested 16 spray-dried AfMNPV determine effect different ingredients (e.g., lignin, corn flour, so on) on after simulated rain (at Peoria, IL) natural exposures Tifton, GA). The most effective formulation...

Bacillus thuringiensis Berliner is a highly efficacious bioinsecticide used to control lepidopteran pests in the field. Unfortunately, it has limited residual activity on plants because sunlight inactivates spores and crystals they can be washed off by rain. To minimize loss of activity, formulations must contain UV protectants, stickers, or both. We tested approximately 80 determined optimal combinations ingredients spray drying conditions for improving B. after simulated rain sunlight....

Microbial entomopathogen–based bioinsecticides are recognized as alternatives to synthetic pesticides. Insects defend themselves against microbial pathogens by innate mechanisms, including increased phenoloxidase (PO) activity, but its relationship with efficacy is little known. This study evaluated the differences in PO activity at different developmental stages of tobacco budworm Heliothis virescens Fabricius (Lepidoptera: Noctuidae) , Indian meal moth Plodia interpunctella (Hübner)...

Microalgae and probiotics such as Bifidobacterium Lactobacillus genera are associated with human beneficial effects. The aim of this study was to evaluate the prebiotic activity Chlorella sorokiniana in longum plantarum viability incorporated into a dairy product (flan) its antiviral effect against rotavirus, which is one major diarrhea-causing pathogens worldwide. Microalge were isolated from Mexican river characterized by molecular tools. Their evaluted determining shelf-life after...

Ruta chalepensis is an herb used to treat various ailments, and its potential cytotoxic effects on different tumor cell lines have been extensively studied. The present study aimed evaluate the activity of R. methanol extract (RCME), sub-partitions obtained from solvents increasing polarity, major compounds, as well their hemolytic, anti-hemolytic, antioxidant potential. in vitro against human hepatocarcinoma (HEP-G2) murine lymphoma line (L5178Y-R) was evaluated using colorimetric...

Plantago major (PM), also known as plantain, is a weed found in temperate zones worldwide. PM leaves have been associated with various biological properties ranging from antiinflammatory, antimicrobial and antitumour to wound healing. However, its mechanism of action boosting the immune function remains be elucidated. We that endotoxin-free methanol extracts leaves, at doses 50, 100, 250, 500 µg/mL, were 4.4 ± 1, 6 12 0.4, 18 0.4-fold increases nitric oxide (NO) production, increased TNF-α...

Dengue is a viral disease transmitted by Aedes mosquitoes. It threat for public health worldwide and its primary vector aegypti becoming resistant to chemical insecticides. These factors have encouraged studies evaluate entomopathogenic fungi against the vector. Here we evaluated mortality, infection, insemination fecundity rates in A. females after infection autodissemination with two Mexican strains of Metarhizium anisopliae. Two M. anisopliae were tested: The Ma-CBG-1 least virulent (lv),...