A5036350104- Ubiquitin and proteasome pathways
- Microfluidic and Bio-sensing Technologies
- Genomics and Chromatin Dynamics
- Microbial Inactivation Methods
- 3D Printing in Biomedical Research
- Pectus Deformity Diagnosis and Treatment
- Planarian Biology and Electrostimulation
- Pluripotent Stem Cells Research
- Restraint-Related Deaths
- Polysaccharides and Plant Cell Walls
- Nerve injury and regeneration
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Biofuel production and bioconversion
- Classical Studies and Legal History
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Railway Engineering and Dynamics
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inflammasome and immune disorders
- Genetics and Neurodevelopmental Disorders
- Otitis Media and Relapsing Polychondritis
- Cancer-related Molecular Pathways
Material Measurement Laboratory
2023-2024
National Institute of Dental and Craniofacial Research
2020-2023
National Institutes of Health
2020-2023
Old Dominion University
2012-2018
Virginia Tech
2017
Adult-onset inflammatory syndromes often manifest with overlapping clinical features. Variants in ubiquitin-related genes, previously implicated autoinflammatory disease, may define new disorders.We analyzed peripheral-blood exome sequence data independent of phenotype and inheritance pattern to identify deleterious mutations genes. Sanger sequencing, immunoblotting, immunohistochemical testing, flow cytometry, transcriptome cytokine profiling were performed. CRISPR-Cas9-edited zebrafish...
Background Therapeutic delivery of angiogenic growth factors is a promising approach for treating ischemia observed in skin flaps and chronic wounds.Several studies have demonstrated that vascular endothelial factor(VEGF) helps mitigate flap necrosis by facilitating angiogenesis. The present study aimed to demonstrate an electrically-mediated nonviral gene using non-invasive multi-electrode array (MEA) effective treatment ischemic flaps.Methods We used standard random dorsal model rats....
Reversible modification of proteins with linkage-specific ubiquitin chains is critical for intracellular signaling. Information on physiological roles and underlying mechanisms particular linkages during human development are limited. Here, relying genomic constraint scores, we identify 10 patients multiple congenital anomalies caused by hemizygous variants in OTUD5, encoding a K48/K63 deubiquitylase. By studying these mutations, find that OTUD5 controls neuroectodermal differentiation...
Abstract Cells are often subjected to the effect of reactive oxygen species (ROS) as a result both intracellular metabolism and exposure exogenous factors. ROS-dependent oxidative stress can induce 8-oxodG within GGG triplet found in G-rich human telomeric sequence (TTAGGG), making telomeres highly susceptible ROS-induced damage. Telomeres nucleoprotein complexes that protect ends linear chromosomes their dysfunction is believed affect wide range cellular and/or organismal processes. Acute...
Chondrocytes are the uniquely resident cells found in all types of cartilage and key to their function is ability respond mechanical loads with changes metabolic activity. This mechanotransduction property is, part, mediated through activity a range expressed transmembrane channels; ion channels, gap junction proteins, porins. Appropriate expression channels has been shown essential for production extracellular matrix differential correlated musculoskeletal diseases such as osteoarthritis...
To facilitate the characterization of unlabeled induced pluripotent stem cells (iPSCs) during culture and expansion, we developed an AI pipeline for nuclear segmentation mitosis detection from phase contrast images individual within iPSC colonies. The analysis uses a 2D convolutional neural network (U-Net) plus 3D U-Net applied on time lapse to detect segment nuclei, mitotic events, daughter nuclei enable tracking large numbers over long times in culture. fluorescence data train models...
Electromanipulation of cells as a label-free cell manipulation and characterization tool has gained particular interest recently. However, the applicability electromanipulation, particularly dielectrophoresis (DEP), to biological is limited suspended in buffers containing lower amounts salts relative physiological buffers. One might question use low conductivity (LCBs) for DEP separation, are stressed lacking levels salt. In LCB, leak ions undergo volume regulation. Therefore, exhibit...
Costal cartilage is much understudied compared with the load-bearing cartilages. Abnormally grown costal cartilages are associated inherited chest wall deformities pectus excavatum and carinatum resulting in sunken pigeon chests, respectively. A lack of understanding ultrastructural molecular biology a major confounder predicting causes outcomes these disorders. This study analyzed structure marginal human (ribs 6–10) through scanning electron atomic force microscopes identified presence...
Abstract The molecular mechanisms that coordinate patterning of the embryonic ectoderm into spatially distinct lineages to form nervous system, epidermis, and neural crest-derived craniofacial structures are unclear. Here, biochemical disease-variant profiling reveals a posttranslational pathway drives early ectodermal differentiation in vertebrate head. anteriorly expressed ubiquitin ligase CRL3-KLHL4 restricts signaling ubiquitous cytoskeletal regulator CDC42. This regulation relies on...
Embryonic development occurs through commitment of pluripotent stem cells to differentiation programs that require highly coordinated changes in gene expression. Chromatin remodeling regulatory elements is a critical component how such are achieved. While many factors controlling chromatin dynamics known, mechanisms different regulators orchestrated during not well understood. Here, we describe LINKED ( LINK age-specific-deubiquitylation-deficiency-induced E mbryonic D efects) syndrome,...
Abstract To facilitate the characterization of unlabeled induced pluripotent stem cells (iPSCs) during culture and expansion, we developed an AI pipeline for nuclear segmentation mitosis detection from phase contrast images individual within iPSC colonies. The analysis uses a 2D convolutional neural network (U-Net) plus 3D U-Net applied on time lapse to detect segment nuclei, mitotic events, daughter nuclei enable tracking large numbers over long times in culture. fluorescence data train...
Chondrocytes form permanent cartilagein joints and ribs. Although much work has been undertaken on joint cartilage with respect to osteoarthritis, little is known about rib, or costal, cartilage. Inherited disorders of costal include pectus excavatum(PE) carinatum (PC), where the sternum either depressed towards spine, protrudes outwardly forming a ‘pigeon chest’ respectively. In severe case there are profound consequences heart lung function. The occurrence these approximately 1 in...
The molecular mechanisms that coordinate patterning of the embryonic ectoderm into spatially distinct lineages to form nervous system, epidermis, and craniofacial structures are unclear. Here, biochemical disease-variant profiling reveals a posttranslational pathway drives early ectodermal differentiation in vertebrate head. anteriorly expressed ubiquitin ligase CRL3-KLHL4 restricts signaling ubiquitous cytoskeletal regulator CDC42. major substrate is canonical CDC42 effector kinase PAK1...